Browse dbCAN-PUL Entries

PULID	Characterization Method(s)	Substrate	Organism	Publication	Publish Date	Type	Num Genes	Num CAZymes	CazyFamily
PUL0013	Northern Blot, RT-PCR, isothermal titration calorimetry, electrophoretic mobility shift assay	arabinan	Geobacillus stearothermophilus	21460081 The L-Arabinan utilization system of Geobacillus stearothermophilus. J Bacteriol. 2011 Jun;193(11):2838-50. doi: 10.1128/JB.00222-11. Epub 2011 Apr 1.	2011 Jun	degradation	56	11	GH43_5, GH43, GH43, GH43_4, GH51, GH51, GH127, CE4, GH52, GH10, GH67, GH39, GH43_11
PUL0148	sequence homology analysis	carrageenan	Zobellia galactanivorans	30524390 Evolutionary Evidence of Algal Polysaccharide Degradation Acquisition by Pseudoalteromonas carrageenovora 9(T) to Adapt to Macroalgal Niches. Front Microbiol. 2018 Nov 22;9:2740. doi: 10.3389/fmicb.2018.02740. eCollection 2018.	2018	degradation	15	4	GH127, GH127, GH127, GH129
PUL0309	enzyme activity assay, substrate binding assay, isothermal titration calorimetry	arabinan	Caldanaerobius polysaccharolyticus	28710263 Enzymatic Mechanism for Arabinan Degradation and Transport in the Thermophilic Bacterium Caldanaerobius polysaccharolyticus. Appl Environ Microbiol. 2017 Aug 31;83(18):e00794-17. doi: 10.1128/AEM.00794-17. Print 2017 Sep 15.	2017 Sep 15	degradation	12	6	GH43_4, GH43, GH127, GH51, GH27, GH51, GH146
PUL0328	microarray, gas chromatography and mass spectrometry	xylan	Gramella flava	28261179 Characterization of Potential Polysaccharide Utilization Systems in the Marine Bacteroidetes Gramella Flava JLT2011 Using a Multi-Omics Approach. Front Microbiol. 2017 Feb 14;8:220. doi: 10.3389/fmicb.2017.00220. eCollection 2017.	2017	degradation	10	4	GH127, CBM13, GH43, GH43_26, GH2, GH5, GH5_13
PUL0332	fosmid library screen, enzyme activity assay, thin layer chromatography	carboxymethylcellulose, xylan, beta-glucan, lichenan	uncultured bacterium	28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.	2017 Jan 16	degradation	19	9	GH5, GH5_2, GH5_7, GH5, CE7, GH2, GH94, GH97, GH127, GH127
PUL0395	isothermal calorimetric titration, electrophoretic mobility shift assay, Northern Blot	arabinose, arabinan	Geobacillus stearothermophilus	21460081 The L-Arabinan utilization system of Geobacillus stearothermophilus. J Bacteriol. 2011 Jun;193(11):2838-50. doi: 10.1128/JB.00222-11. Epub 2011 Apr 1.	2011 Jun	degradation	25	5	GH43, GH43_5, GH43, GH43_4, CBM54, GH51, GH51, GH127
PUL0460	recombinant protein expression, RT-PCR, enzyme activity assay	carrageenan, agar	Paraglaciecola hydrolytica	29774012 A Novel Enzyme Portfolio for Red Algal Polysaccharide Degradation in the Marine Bacterium Paraglaciecola hydrolytica S66(T) Encoded in a Sizeable Polysaccharide Utilization Locus. Front Microbiol. 2018 May 3;9:839. doi: 10.3389/fmicb.2018.00839. eCollection 2018.	2018	degradation	108	18	GH2, GH29, GH63, GH86, CBM6, GH50, GH86, GH50, GH117, GH50, GH16, GH50, CE1, GH16, GH127, GH16, GH82, GH16, GH16, GH16
PUL0482	growth assay	pectin	Flavobacterium johnsoniae	19717629 Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28.	2009 Nov	degradation	27	17	GH78, CBM67, GH142, GH95, GH78, CBM67, GH143, GH43_18, GH43, GH28, GH140, PL1, CE8, PL1_2, PL1, CE8, PL1_2, CE8, GH127, PL1, PL1_2, GH139, GH106, GH2, GH137, GH78
PUL0530	microarray, qPCR	rhamnogalacturonan	Bacteroides thetaiotaomicron	22205877 Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.	2011 Dec	degradation	54	21	PL1, CE8, PL1_2, GH2, GH139, GH106, GH2, GH2, GH137, GH2, CBM57, GH138, GH78, GH141, GH127, GH95, GH105, GH140, GH78, GH33, CBM67, GH28, GH78, CBM67, GH143, GH142, GH43_18, GH43, PL1, CE8, PL1_2
PUL0558	gene deletion mutant and growth assay, growth assay, enzyme activity assay	rhamnogalacturonan	Bacteroides thetaiotaomicron	28329766 Complex pectin metabolism by gut bacteria reveals novel catalytic functions. Nature. 2017 Apr 6;544(7648):65-70. doi: 10.1038/nature21725. Epub 2017 Mar 22.	2017 Apr 6	degradation	50	20	GH2, GH139, GH106, GH2, GH2, GH2, CBM57, GH137, GH138, GH78, GH141, GH127, GH95, GH105, GH140, CBM67, GH33, GH78, GH28, CBM67, GH78, GH142, GH143, GH43_18, GH43, CE8, PL1_2, PL1
PUL0563	microarray, qPCR	arabinogalactan	Bacteroides thetaiotaomicron	18996345, 22205877 Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.	2008 Nov 13,2011 Dec	degradation	14	7	GH127, GH43, GH43_34, GH154, GH43, GH16, GH43_24, GH43_24, GH145, GH105
PUL0626	high performance anion exchange chromatography	arabinan	Lactobacillus crispatus DSM29598	33119797 Characterization of two extracellular arabinanases in Lactobacillus crispatus. Appl Microbiol Biotechnol. 2020 Dec;104(23):10091-10103. doi: 10.1007/s00253-020-10979-0. Epub 2020 Oct 29.	2020 Dec	degradation	24	8	GH43, CBM54, GH43_4, GH2, GH43, GH43_4, GH127, GH51, GH43, CBM42, GH43_26, GH51, GH27, CBM51
PUL0651	enzyme activity assay, NMR	agar	Gilvimarinus chinensis DSM 19667	33691998 Agarase cocktail from agar polysaccharide utilization loci converts homogenized Gelidium amansii into neoagarooligosaccharides. Food Chem. 2021 Aug 1;352:128685. doi: 10.1016/j.foodchem.2020.128685. Epub 2020 Nov 19.	2021 Aug 1	degradation	63	14	CBM6, GH16_16, GH16, GH50, CBM6, CBM13, CBM6, GH16_16, GH16, GH50, GH117, GH16_3, GH16, GH2, GH50, GH127, GH167, CBM2, CBM6, GH86, 3.2.1.81, GH50, 3.2.1.81, GH86