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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0009

PubMed

28432302, Sci Rep. 2017 Apr 21;7(1):1069. doi: 10.1038/s41598-017-00917-y.

Characterization method

enzyme activity assay,immunoblotting,ATPase assay

Genomic accession number

NC_013515.1

Nucelotide position range

121344-139674

Substrate

glycosaminoglycan

Loci

SMON_RS00575-SMON_RS00640

Species

Streptobacillus moniliformis/34105

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- SMON_RS00570 WP_012858165.1 0 - 4 (+) NC_013515.1:121344-121348 -
- SMON_RS00575 WP_012858166.1 0 - 942 (+) NC_013515.1:121344-122286 -
eda SMON_RS00580 WP_041794192.1 952 - 1600 (+) NC_013515.1:122296-122944 4.1.2.14, 4.1.3.16
- SMON_RS00585 WP_012858168.1 1648 - 3505 (+) NC_013515.1:122992-124849 4.2.2.1
kduD SMON_RS00590 WP_041794195.1 3518 - 4268 (+) NC_013515.1:124862-125612 1.1.1.127
kduI SMON_RS00595 WP_012858170.1 4286 - 5123 (+) NC_013515.1:125630-126467 5.3.1.17
ugpC SMON_RS00600 WP_012858171.1 5133 - 6234 (+) NC_013515.1:126477-127578 -
- SMON_RS00605 WP_012858172.1 6243 - 7140 (+) NC_013515.1:127587-128484 -
- SMON_RS00610 WP_012858173.1 7149 - 8028 (+) NC_013515.1:128493-129372 -
- SMON_RS00615 WP_012858174.1 8054 - 9572 (+) NC_013515.1:129398-130916 -
- SMON_RS00620 WP_041793794.1 9616 - 11413 (+) NC_013515.1:130960-132757 -
- SMON_RS00625 WP_012858176.1 11409 - 13629 (+) NC_013515.1:132753-134973 -
- SMON_RS00630 WP_012858177.1 13641 - 15045 (+) NC_013515.1:134985-136389 -
- SMON_RS00635 WP_012858178.1 15058 - 16243 (+) NC_013515.1:136402-137587 -
- SMON_RS00640 WP_012858179.1 16252 - 18331 (+) NC_013515.1:137596-139675 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 4 (+) TF: DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955 No
- 1 - 942 (+) STP: STP|PfkB No
eda 953 - 1600 (+) CDS No
- 1649 - 3505 (+) CAZyme: PL8 Yes
kduD 3519 - 4268 (+) other Yes
kduI 4287 - 5123 (+) other Yes
ugpC 5134 - 6234 (+) TC: gnl|TC-DB|Q48394|3.A.1.1.6 Yes
- 6244 - 7140 (+) TC: gnl|TC-DB|Q9KWT8|3.A.1.1.10 Yes
- 7150 - 8028 (+) TC: gnl|TC-DB|Q9KWT7|3.A.1.1.10 Yes
- 8055 - 9572 (+) TC: gnl|TC-DB|Q9KWT6|3.A.1.1.10 Yes
- 9617 - 11413 (+) other Yes
- 11410 - 13629 (+) CAZyme: PL8 Yes
- 13642 - 15045 (+) other Yes
- 15059 - 16243 (+) CAZyme: GH88 Yes
- 16253 - 18331 (+) CDS No

PUL ID

PUL0009

PubMed

28432302, Sci Rep. 2017 Apr 21;7(1):1069. doi: 10.1038/s41598-017-00917-y.

Title

A bacterial ABC transporter enables import of mammalian host glycosaminoglycans.

Author

Oiki S, Mikami B, Maruyama Y, Murata K, Hashimoto W

Abstract

Glycosaminoglycans (GAGs), such as hyaluronan, chondroitin sulfate, and heparin, constitute mammalian extracellular matrices. The uronate and amino sugar residues in hyaluronan and chondroitin sulfate are linked by 1,3-glycoside bond, while heparin contains 1,4-glycoside bond. Some bacteria target GAGs as means of establishing colonization and/or infection, and bacterial degradation mechanisms of GAGs have been well characterized. However, little is known about the bacterial import of GAGs. Here, we show a GAG import system, comprised of a solute-binding protein (Smon0123)-dependent ATP-binding cassette (ABC) transporter, in the pathogenic Streptobacillus moniliformis. A genetic cluster responsible for depolymerization, degradation, and metabolism of GAGs as well as the ABC transporter system was found in the S. moniliformis genome. This bacterium degraded hyaluronan and chondroitin sulfate with an expression of the genetic cluster, while heparin repressed the bacterial growth. The purified recombinant Smon0123 exhibited an affinity with disaccharides generated from hyaluronan and chondroitin sulfate. X-ray crystallography indicated binding mode of Smon0123 to GAG disaccharides. The purified recombinant ABC transporter as a tetramer (Smon0121-Smon0122/Smon0120-Smon0120) reconstructed in liposomes enhanced its ATPase activity in the presence of Smon0123 and GAG disaccharides. This is the first report that has molecularly depicted a bacterial import system of both sulfated and non-sulfated GAGs.