PUL ID

PUL0009

PubMed

28432302, Sci Rep. 2017 Apr 21;7(1):1069. doi: 10.1038/s41598-017-00917-y.

Characterization method

enzyme activity assay,immunoblotting,ATPase assay

Genomic accession number

NC_013515.1

Nucelotide position range

121344-139674

Substrate

glycosaminoglycan

Loci

SMON_RS00575-SMON_RS00640

Species

Streptobacillus moniliformis/34105

Degradation or Biosynthesis

degradation

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 4 (+) TF: DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955 No
- 1 - 942 (+) STP: STP|PfkB No
eda 953 - 1600 (+) CDS No
- 1649 - 3505 (+) CAZyme: PL8 Yes
kduD 3519 - 4268 (+) other Yes
kduI 4287 - 5123 (+) other Yes
ugpC 5134 - 6234 (+) TC: gnl|TC-DB|Q48394|3.A.1.1.6 Yes
- 6244 - 7140 (+) TC: gnl|TC-DB|Q9KWT8|3.A.1.1.10 Yes
- 7150 - 8028 (+) TC: gnl|TC-DB|Q9KWT7|3.A.1.1.10 Yes
- 8055 - 9572 (+) TC: gnl|TC-DB|Q9KWT6|3.A.1.1.10 Yes
- 9617 - 11413 (+) other Yes
- 11410 - 13629 (+) CAZyme: PL8 Yes
- 13642 - 15045 (+) other Yes
- 15059 - 16243 (+) CAZyme: GH88 Yes
- 16253 - 18331 (+) CDS No

PUL ID

PUL0009

PubMed

28432302, Sci Rep. 2017 Apr 21;7(1):1069. doi: 10.1038/s41598-017-00917-y.

Title

A bacterial ABC transporter enables import of mammalian host glycosaminoglycans.

Author

Oiki S, Mikami B, Maruyama Y, Murata K, Hashimoto W

Abstract

Glycosaminoglycans (GAGs), such as hyaluronan, chondroitin sulfate, and heparin, constitute mammalian extracellular matrices. The uronate and amino sugar residues in hyaluronan and chondroitin sulfate are linked by 1,3-glycoside bond, while heparin contains 1,4-glycoside bond. Some bacteria target GAGs as means of establishing colonization and/or infection, and bacterial degradation mechanisms of GAGs have been well characterized. However, little is known about the bacterial import of GAGs. Here, we show a GAG import system, comprised of a solute-binding protein (Smon0123)-dependent ATP-binding cassette (ABC) transporter, in the pathogenic Streptobacillus moniliformis. A genetic cluster responsible for depolymerization, degradation, and metabolism of GAGs as well as the ABC transporter system was found in the S. moniliformis genome. This bacterium degraded hyaluronan and chondroitin sulfate with an expression of the genetic cluster, while heparin repressed the bacterial growth. The purified recombinant Smon0123 exhibited an affinity with disaccharides generated from hyaluronan and chondroitin sulfate. X-ray crystallography indicated binding mode of Smon0123 to GAG disaccharides. The purified recombinant ABC transporter as a tetramer (Smon0121-Smon0122/Smon0120-Smon0120) reconstructed in liposomes enhanced its ATPase activity in the presence of Smon0123 and GAG disaccharides. This is the first report that has molecularly depicted a bacterial import system of both sulfated and non-sulfated GAGs.