PUL ID

PUL0038

PubMed

10741834, Extremophiles. 2000 Feb;4(1):23-33. doi: 10.1007/s007920050004.

Characterization method

enzyme activity assay, Southern Blot

Genomic accession number

AF135398.1

Nucelotide position range

1-10050

Substrate

melibiose,galactose

Loci

bdtEFG-bgaT-agaT-galT-glpK-glpD

Species

Thermus brockianus/56956

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

bdtE - AAD33664.1 0 - 906 (+) AF135398.1:1-907 -
bdtF - AAD33665.1 954 - 1797 (+) AF135398.1:955-1798 -
bdtG - AAD33666.1 1793 - 2624 (+) AF135398.1:1794-2625 -
bgaT - AAD33667.1 2628 - 4566 (+) AF135398.1:2629-4567 -
agaT - AAD33668.1 4569 - 6000 (+) AF135398.1:4570-6001 -
galT - AAD33669.1 5983 - 7030 (+) AF135398.1:5984-7031 -
glpK - AAD33670.1 7026 - 8514 (+) AF135398.1:7027-8515 -
glpD - AAD33671.1 8514 - 10050 (+) AF135398.1:8515-10051 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 906 (+) STP: STP|SBP_bac_1 No
- 955 - 1797 (+) TC: gnl|TC-DB|P29823|3.A.1.1.4 Yes
- 1794 - 2624 (+) TC: gnl|TC-DB|P94530|3.A.1.1.34 Yes
- 2629 - 4566 (+) CAZyme: GH42 Yes
- 4570 - 6000 (+) CAZyme: GH36 Yes
- 5984 - 7030 (+) CDS No
- 7027 - 8514 (+) CDS No
- 8515 - 10050 (+) CDS No

PUL ID

PUL0038

PubMed

10741834, Extremophiles. 2000 Feb;4(1):23-33. doi: 10.1007/s007920050004.

Title

The structure of the alpha-galactosidase gene loci in Thermus brockianus ITI360 and Thermus thermophilus TH125.

Author

Fridjonsson O, Watzlawick H, Mattes R

Abstract

The Thermus thermophilus TH125 alpha-galactosidase gene, agaT, and flanking sequences were cloned in Escherichia coli and sequenced as well as flanking sequences of the previously cloned agaT from Thermus brockianus ITI360. Different structures of putative alpha-galactosidase operons in the two Thermus strains were revealed. Downstream of and overlapping with the alpha-galactosidase genes of both strains, a gene was identified that is similar to the galactose-1-phosphate uridylyltransferase gene (galT) of E. coli and Streptomyces lividans. Upstream of the agaT of T. brockianus ITI360, four open reading frames were observed. The deduced translation products displayed similarity to components of bacterial binding protein-dependent transport systems and a beta-galactosidase. No galactoside utilization genes were identified upstream of agaT in T. thermophilus TH125. The inactivation of the alpha-galactosidase genes of both strains by insertional mutagenesis led to an inability to use melibiose or galactose as a single carbohydrate source. An attempt was made to isolate a gene encoding the enzyme responsible for para-nitrophenyl-(pNP-) beta-galactoside hydrolyzing activity in T. thermophilus TH125. A gene designated bglT was cloned and expressed in E. coli. The inactivation of the bglT gene led to 55% reduction of the pNP-beta-galactoside hydrolyzing activity in the mutant strain in comparison to the wild type.