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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0090

PubMed

31220629, Carbohydr Res. 2019 Jul 15;481:9-15. doi: 10.1016/j.carres.2019.06.001. Epub 2019 Jun 6.

Characterization method

sequence homology analysis,sugar utilization assay,NMR

Genomic accession number

EU294173.1

Nucelotide position range

1085-13333

Substrate

O-antigen

Loci

wffw+ wffx+ orf3+ orf4+ wzx+ wzy+ orf7+ wbub+ fnla+ fnlb+ fnlc+ wbuc+ wzz

Species

Escherichia coli/562

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

wffW - ACA24847.1 1085 - 1481 (+) EU294173.1:2170-2566 -
wffX - ACA24848.1 1477 - 2638 (+) EU294173.1:2562-3723 -
wffY - ACA24849.1 2634 - 3735 (+) EU294173.1:3719-4820 -
wffZ - ACA24850.1 3721 - 4777 (+) EU294173.1:4806-5862 -
wzx - ACA24851.1 4776 - 6177 (+) EU294173.1:5861-7262 -
wzy - ACA24852.1 6173 - 7337 (+) EU294173.1:7258-8422 -
wfgA - ACA24853.1 7333 - 8443 (+) EU294173.1:8418-9528 -
fnlA - ACA24854.1 8467 - 9502 (+) EU294173.1:9552-10587 -
fnlB - ACA24855.1 9494 - 10607 (+) EU294173.1:10579-11692 -
fnlC - ACA24856.1 10606 - 11737 (+) EU294173.1:11691-12822 -
wbuB - ACA24857.1 11736 - 12948 (+) EU294173.1:12821-14033 -
wbuC - ACA24858.1 12934 - 13333 (+) EU294173.1:14019-14418 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1086 - 1481 (+) CDS No
- 1478 - 2638 (+) CDS No
- 2635 - 3735 (+) CAZyme: GT4 Yes
- 3722 - 4777 (+) CAZyme: GT4 Yes
- 4777 - 6177 (+) other Yes
- 6174 - 7337 (+) other Yes
- 7334 - 8443 (+) other Yes
- 8468 - 9502 (+) TC: gnl|TC-DB|Q6MMD5|9.B.18.2.1 Yes
- 9495 - 10607 (+) other Yes
- 10607 - 11737 (+) other Yes
- 11737 - 12948 (+) CAZyme: GT4 Yes
- 12935 - 13333 (+) CDS No

PUL ID

PUL0090

PubMed

31220629, Carbohydr Res. 2019 Jul 15;481:9-15. doi: 10.1016/j.carres.2019.06.001. Epub 2019 Jun 6.

Title

Structure elucidation and gene cluster characterization of the O-antigen of Yersinia kristensenii capital ES, Cyrillic-134.

Author

Sizova OV, Shashkov AS, Toukach PV, Knirel YA, Shaikhutdinova RZ, Ivanov SA, Kislichkina AA, Dentovskaya SV

Abstract

Mild acid degradation of the lipopolysaccharide of Yersinia kristensenii C-134 afforded a glycerol teichoic acid-like O-polysaccharide, which was studied by sugar analysis, O-deacetylation and dephosphorylation along with 1D and 2D NMR spectroscopy. The following structure of the O-polysaccharide was established: This structure is related to those of other Y. kristensenii O-polysaccharides studied earlier. The O-antigen gene cluster of Y. kristensenii capital ES, Cyrillic-134 was analyzed and found to be consistent with the O-polysaccharide structure established.