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PlantCAZyme
Cite: NAR/gkaa742 2020

Because CGCFinder predicted no CGC for this PUL, the gene cluster depicted below contains dbCAN2 and CGC signature predictions for all genes in the PUL, instead of a predicted CGC.


PUL General Information

Literature Information

PUL ID

PUL0115

PubMed

31160824, Nat Microbiol. 2019 Sep;4(9):1571-1581. doi: 10.1038/s41564-019-0466-x. Epub 2019 Jun 3.

Characterization method

recombinant protein expression,RNA-Seq,differential gene expression

Genomic accession number

AE015928.1

Nucelotide position range

557455-572833

Substrate

N-glycan

Loci

BT_0455-BT_0461

Species

Bacteroides thetaiotaomicron/818

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BT_0455 AAO75562.1 0 - 1635 (+) AE015928.1:557455-559090 -
- BT_0456 AAO75563.1 1631 - 3644 (+) AE015928.1:559086-561099 -
- BT_0457 AAO75564.1 3655 - 5734 (+) AE015928.1:561110-563189 -
- BT_0458 AAO75565.1 5811 - 8406 (+) AE015928.1:563266-565861 -
- BT_0459 AAO75566.1 8444 - 10769 (+) AE015928.1:565899-568224 -
- BT_0460 AAO75567.1 10768 - 12844 (+) AE015928.1:568223-570299 -
- BT_0461 AAO75568.1 12859 - 15379 (+) AE015928.1:570314-572834 -

Cluster number

0

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1635 (+) CAZyme: GH33 No
- 1632 - 3644 (+) CAZyme: GH20 No
- 3656 - 5734 (+) CDS No
- 5812 - 8406 (+) CAZyme: GH2 No
- 8445 - 10769 (+) CAZyme: GH20 No
- 10769 - 12844 (+) CAZyme: GH20 No
- 12860 - 15379 (+) CAZyme: GH2 No

PUL ID

PUL0115

PubMed

31160824, Nat Microbiol. 2019 Sep;4(9):1571-1581. doi: 10.1038/s41564-019-0466-x. Epub 2019 Jun 3.

Title

Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci.

Author

Briliute J, Urbanowicz PA, Luis AS, Basle A, Paterson N, Rebello O, Hendel J, Ndeh DA, Lowe EC, Martens EC, Spencer DIR, Bolam DN, Crouch LI

Abstract

Glycans are the major carbon sources available to the human colonic microbiota. Numerous N-glycosylated proteins are found in the human gut, from both dietary and host sources, including immunoglobulins such as IgA that are secreted into the intestine at high levels. Here, we show that many mutualistic gut Bacteroides spp. have the capacity to utilize complex N-glycans (CNGs) as nutrients, including those from immunoglobulins. Detailed mechanistic studies using transcriptomic, biochemical, structural and genetic techniques reveal the pathway employed by Bacteroides thetaiotaomicron (Bt) for CNG degradation. The breakdown process involves an extensive enzymatic apparatus encoded by multiple non-adjacent loci and comprises 19 different carbohydrate-active enzymes from different families, including a CNG-specific endo-glycosidase activity. Furthermore, CNG degradation involves the activity of carbohydrate-active enzymes that have previously been implicated in the degradation of other classes of glycan. This complex and diverse apparatus provides Bt with the capacity to access the myriad different structural variants of CNGs likely to be found in the intestinal niche.