PUL ID

PUL0130

PubMed

30385338, Int J Biol Macromol. 2019 Feb 1;122:555-561. doi: 10.1016/j.ijbiomac.2018.10.189. Epub 2018 Oct 29.

Characterization method

NMR, mass spectrometry

Genomic accession number

MWTN01000002.1

Nucelotide position range

83188-99693

Substrate

O-antigen

Loci

manC-manB-wzm-wzt-wejH-wejI-gmd-rmd-wejJ-wejK-wejL-wejM

Species

Yersinia rohdei/29485

Degradation or Biosynthesis

biosynthesis

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 14 - 1429 (+) STP: STP|AraC_binding No
- 2830 - 3615 (+) TC: gnl|TC-DB|Q9HTB8|3.A.1.103.6 Yes
- 3615 - 4841 (+) TC: gnl|TC-DB|Q9I3V8|3.A.1.103.6 Yes
- 4900 - 6048 (+) other Yes
- 6125 - 6676 (+) other Yes
- 6801 - 7217 (+) CAZyme: GT4 Yes
- 8652 - 9689 (+) other Yes
- 9689 - 10585 (+) other Yes
- 11724 - 12839 (+) CAZyme: GT4 Yes
- 13092 - 13973 (+) CDS No
- 15167 - 16506 (+) CDS No

PUL ID

PUL0130

PubMed

30385338, Int J Biol Macromol. 2019 Feb 1;122:555-561. doi: 10.1016/j.ijbiomac.2018.10.189. Epub 2018 Oct 29.

Title

Structure and gene cluster of the O-polysaccharide of Yersinia rohdei H274-36/78.

Author

Sizova OV, Shashkov AS, Dmitrenok AS, Toukach PV, Knirel YA, Shaikhutdinova RZ, Ivanov SA, Kislichkina AA, Bogun AG, Dentovskaya SV

Abstract

A branched O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Yersinia rohdei H274-36/78 and found to contain d-rhamnose, d-mannose, and 3,6-dideoxy-4-C-[(S)-1-hydroxyethyl]-d-xylo-hexose called yersiniose A (Yer). Partial acid hydrolysis of the O-polysaccharide eliminated Yer residues to give a modified linear polysaccharide. Studies by sugar analysis and (1)H and (13)C NMR spectroscopy, including computational NMR analysis, enabled structure elucidation of a hexasaccharide repeating unit of the O-polysaccharide having two Yer residues attached as monosaccharide side chains. The O-antigen gene cluster of Y. rohdei H274-36/78 located between JUMPStart and galF genes contained putative genes for synthesis of precursors of two O-antigen constituents, GDP-d-Man and GDP-d-Rha, whereas genes responsible for synthesis of CDP-Yer were within the chromosome outside the O-antigen gene cluster. Glycosyltransferase genes and ABC 2 transporter genes were present in the O-antigen gene cluster, and hence the structure established is consistent with the polysaccharide synthesis gene content of the genome.