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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0167

PubMed

30246424, Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.

Characterization method

mass spectrometry,sequence homology analysis

Genomic accession number

AAXF02000047.1

Nucelotide position range

109596-128600

Substrate

beta-mannan

Loci

BACOVA_02087-BACOVA_02097

Species

Bacteroides ovatus/28116

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BACOVA_02087 EDO12197.1 0 - 201 (-) AAXF02000047.1:109596-109797 -
- BACOVA_02088 EDO12198.1 173 - 1364 (-) AAXF02000047.1:109769-110960 5.1.3.8
- BACOVA_02089 EDO12199.1 1376 - 2750 (-) AAXF02000047.1:110972-112346 -
- BACOVA_02090 EDO12200.1 2779 - 3997 (-) AAXF02000047.1:112375-113593 -
- BACOVA_02091 EDO12201.1 3993 - 6204 (-) AAXF02000047.1:113589-115800 -
- BACOVA_02092 EDO12202.1 6210 - 7311 (-) AAXF02000047.1:115806-116907 -
- BACOVA_02093 EDO12203.1 7328 - 8411 (-) AAXF02000047.1:116924-118007 -
- BACOVA_02094 EDO12204.1 8430 - 9564 (-) AAXF02000047.1:118026-119160 -
- BACOVA_02095 EDO12205.1 9656 - 11468 (-) AAXF02000047.1:119252-121064 -
- BACOVA_02096 EDO12206.1 11494 - 14743 (-) AAXF02000047.1:121090-124339 -
- BACOVA_02097 EDO12207.1 15075 - 19005 (+) AAXF02000047.1:124671-128601 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 201 (-) CDS No
- 174 - 1364 (-) CDS No
- 1377 - 2750 (-) TC: gnl|TC-DB|A1S5F2|2.A.2.3.6 Yes
- 2780 - 3997 (-) CAZyme: GH130 Yes
- 3994 - 6204 (-) CAZyme: GH36 Yes
- 6211 - 7311 (-) CAZyme: GH26 Yes
- 7329 - 8411 (-) CAZyme: GH26 Yes
- 8431 - 9564 (-) other Yes
- 9657 - 11468 (-) other Yes
- 11495 - 14743 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 15076 - 19005 (+) TF: DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0036286,DBD-SUPERFAMILY|0035607 No
- 18979 - 19005 (-) CDS No

PUL ID

PUL0167

PubMed

30246424, Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.

Title

Alpha- and beta-mannan utilization by marine Bacteroidetes.

Author

Chen J, Robb CS, Unfried F, Kappelmann L, Markert S, Song T, Harder J, Avci B, Becher D, Xie P, Amann RI, Hehemann JH, Schweder T, Teeling H

Abstract

Marine microscopic algae carry out about half of the global carbon dioxide fixation into organic matter. They provide organic substrates for marine microbes such as members of the Bacteroidetes that degrade algal polysaccharides using carbohydrate-active enzymes (CAZymes). In Bacteroidetes genomes CAZyme encoding genes are mostly grouped in distinct regions termed polysaccharide utilization loci (PULs). While some studies have shown involvement of PULs in the degradation of algal polysaccharides, the specific substrates are for the most part still unknown. We investigated four marine Bacteroidetes isolated from the southern North Sea that harbour putative mannan-specific PULs. These PULs are similarly organized as PULs in human gut Bacteroides that digest alpha- and beta-mannans from yeasts and plants respectively. Using proteomics and defined growth experiments with polysaccharides as sole carbon sources we could show that the investigated marine Bacteroidetes express the predicted functional proteins required for alpha- and beta-mannan degradation. Our data suggest that algal mannans play an as yet unknown important role in the marine carbon cycle, and that biochemical principles established for gut or terrestrial microbes also apply to marine bacteria, even though their PULs are evolutionarily distant.