PUL ID

PUL0179

PubMed

27288925, FEBS Lett. 2016 Jul;590(14):2106-18. doi: 10.1002/1873-3468.12250. Epub 2016 Jun 28.

Characterization method

enzyme activity assay, enzyme specificity assay, substrate specificity assay

Genomic accession number

JH724241.1

Nucelotide position range

777587-798381

Substrate

galactomannan

Loci

HMPREF1070_00547-HMPREF1070_00560

Species

Bacteroides ovatus/28116

Degradation or Biosynthesis

degradation

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 840 (-) CDS No
- 868 - 2058 (-) CDS No
- 2072 - 3355 (-) CAZyme: CE7 Yes
- 3363 - 4730 (-) TC: gnl|TC-DB|A1S5F2|2.A.2.3.6 Yes
- 4727 - 5905 (-) CAZyme: GH130 Yes
- 5924 - 6994 (-) CAZyme: GH26 Yes
- 7030 - 8229 (-) CAZyme: GH26 Yes
- 8469 - 9260 (-) other Yes
- 9326 - 10498 (-) other Yes
- 10532 - 12328 (-) other Yes
- 12352 - 15597 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 15653 - 16093 (-) CDS No
- 16126 - 16566 (-) CDS No
- 16803 - 20795 (+) TF: DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0036286,DBD-SUPERFAMILY|0035607 No
- 20792 - 20795 (-) CDS No

PUL ID

PUL0179

PubMed

27288925, FEBS Lett. 2016 Jul;590(14):2106-18. doi: 10.1002/1873-3468.12250. Epub 2016 Jun 28.

Title

A beta-mannan utilization locus in Bacteroides ovatus involves a GH36 alpha-galactosidase active on galactomannans.

Author

Reddy SK, Bagenholm V, Pudlo NA, Bouraoui H, Koropatkin NM, Martens EC, Stalbrand H

Abstract

The Bacova_02091 gene in the beta-mannan utilization locus of Bacteroides ovatus encodes a family GH36 alpha-galactosidase (BoGal36A), transcriptionally upregulated during growth on galactomannan. Characterization of recombinant BoGal36A reveals unique properties compared to other GH36 alpha-galactosidases, which preferentially hydrolyse terminal alpha-galactose in raffinose family oligosaccharides. BoGal36A prefers hydrolysing internal galactose substitutions from intact and depolymerized galactomannan. BoGal36A efficiently releases (> 90%) galactose from guar and locust bean galactomannans, resulting in precipitation of the polysaccharides. As compared to other GH36 structures, the BoGal36A 3D model displays a loop deletion, resulting in a wider active site cleft which likely can accommodate a galactose-substituted polymannose backbone.