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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0180

PubMed

27288925, FEBS Lett. 2016 Jul;590(14):2106-18. doi: 10.1002/1873-3468.12250. Epub 2016 Jun 28.
21539815, Biochem Biophys Res Commun. 2011 May 20;408(4):701-6. doi: 10.1016/j.bbrc.2011.04.095. Epub 2011 Apr 24.

Characterization method

enzyme activity assay

Genomic accession number

CR626927.1

Nucelotide position range

973099-1007603

Substrate

galactomannan

Loci

BF9343_0759-BF9343_0777

Species

Bacteroides fragilis/817

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BF9343_0759 CAH06540.1 0 - 1452 (-) CR626927.1:973099-974551 -
- BF9343_0760 CAH06541.1 1456 - 2797 (-) CR626927.1:974555-975896 -
dxs BF9343_0761 CAH06542.1 2835 - 4782 (-) CR626927.1:975934-977881 2.2.1.7
- BF9343_0762 CAH06543.1 5280 - 6624 (-) CR626927.1:978379-979723 -
- BF9343_0763 CAH06544.1 6620 - 6917 (-) CR626927.1:979719-980016 -
- BF9343_0764 CAH06545.1 7122 - 7770 (+) CR626927.1:980221-980869 3.1.3.18
- BF9343_0765 CAH06546.1 7849 - 9406 (-) CR626927.1:980948-982505 -
- BF9343_0766 CAH06547.1 9426 - 11502 (-) CR626927.1:982525-984601 -
- BF9343_0767 CAH06548.1 11511 - 12894 (-) CR626927.1:984610-985993 -
- BF9343_0768 CAH06549.1 12896 - 14348 (-) CR626927.1:985995-987447 -
- BF9343_0769 CAH06550.1 14462 - 16445 (-) CR626927.1:987561-989544 -
- BF9343_0770 CAH06551.1 16457 - 19643 (-) CR626927.1:989556-992742 -
- BF9343_0771 CAH06552.1 19828 - 23914 (+) CR626927.1:992927-997013 -
- BF9343_0772 CAH06553.1 24080 - 25505 (-) CR626927.1:997179-998604 -
- BF9343_0773 CAH06554.1 25969 - 27319 (+) CR626927.1:999068-1000418 -
- BF9343_0774 CAH06555.1 27337 - 28585 (+) CR626927.1:1000436-1001684 -
- BF9343_0775 CAH06556.1 28905 - 32352 (-) CR626927.1:1002004-1005451 -
- BF9343_0776 CAH06557.1 32462 - 33503 (-) CR626927.1:1005561-1006602 -
- BF9343_0777 CAH06558.1 33710 - 34505 (-) CR626927.1:1006809-1007604 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1452 (-) TC: gnl|TC-DB|P0AFZ7|2.A.38.1.1 Yes
- 1457 - 2797 (-) STP: STP|TrkA_C,STP|TrkA_C Yes
dxs 2836 - 4782 (-) other Yes
- 5281 - 6624 (-) other Yes
- 6621 - 6917 (-) other Yes
- 7123 - 7770 (+) other Yes
- 7850 - 9406 (-) other Yes
- 9427 - 11502 (-) CAZyme: GH36 Yes
- 11512 - 12894 (-) CAZyme: GH29 Yes
- 12897 - 14348 (-) other Yes
- 14463 - 16445 (-) other Yes
- 16458 - 19643 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 19829 - 23914 (+) TF: DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0035607 Yes
- 24081 - 25505 (-) TC: gnl|TC-DB|Q07YH1|2.A.1.14.25 Yes
- 25970 - 27319 (+) CAZyme: GH29 Yes
- 27338 - 28585 (+) other Yes
- 28906 - 32352 (-) other Yes
- 32463 - 33503 (-) TC: gnl|TC-DB|Q643C2|9.B.97.3.1 Yes
- 33711 - 34505 (-) TC: gnl|TC-DB|Q1CZY2|2.A.64.1.2 Yes

PUL ID

PUL0180

PubMed

27288925, FEBS Lett. 2016 Jul;590(14):2106-18. doi: 10.1002/1873-3468.12250. Epub 2016 Jun 28.

Title

A beta-mannan utilization locus in Bacteroides ovatus involves a GH36 alpha-galactosidase active on galactomannans.

Author

Reddy SK, Bagenholm V, Pudlo NA, Bouraoui H, Koropatkin NM, Martens EC, Stalbrand H

Abstract

The Bacova_02091 gene in the beta-mannan utilization locus of Bacteroides ovatus encodes a family GH36 alpha-galactosidase (BoGal36A), transcriptionally upregulated during growth on galactomannan. Characterization of recombinant BoGal36A reveals unique properties compared to other GH36 alpha-galactosidases, which preferentially hydrolyse terminal alpha-galactose in raffinose family oligosaccharides. BoGal36A prefers hydrolysing internal galactose substitutions from intact and depolymerized galactomannan. BoGal36A efficiently releases (> 90%) galactose from guar and locust bean galactomannans, resulting in precipitation of the polysaccharides. As compared to other GH36 structures, the BoGal36A 3D model displays a loop deletion, resulting in a wider active site cleft which likely can accommodate a galactose-substituted polymannose backbone.

PubMed

21539815, Biochem Biophys Res Commun. 2011 May 20;408(4):701-6. doi: 10.1016/j.bbrc.2011.04.095. Epub 2011 Apr 24.

Title

New microbial mannan catabolic pathway that involves a novel mannosylglucose phosphorylase.

Author

Senoura T, Ito S, Taguchi H, Higa M, Hamada S, Matsui H, Ozawa T, Jin S, Watanabe J, Wasaki J, Ito S

Abstract

The consecutive genes BF0771-BF0774 in the genome of Bacteroides fragilis NCTC 9343 were found to constitute an operon. The functional analysis of BF0772 showed that the gene encoded a novel enzyme, mannosylglucose phosphorylase that catalyzes the reaction, 4-O-beta-d-mannopyranosyl-d-glucose+Pi-->mannose-1-phosphate+glucose. Here we propose a new mannan catabolic pathway in the anaerobe, which involves 1,4-beta-mannanase (BF0771), a mannobiose and/or sugar transporter (BF0773), mannobiose 2-epimerase (BF0774), and mannosylglucose phosphorylase (BF0772), finally progressing to glycolysis. This pathway is distributed in microbes such as Bacteroides, Parabacteroides, Flavobacterium, and Cellvibrio.