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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0188

PubMed

26582605, Glycobiology. 2016 Apr;26(4):335-42. doi: 10.1093/glycob/cwv106. Epub 2015 Nov 17.

Characterization method

NMR,sugar utilization assay

Genomic accession number

GU068045.1

Nucelotide position range

107-11174

Substrate

O-antigen

Loci

wcnP-wcnQ-wcnR-wcnS-wcnT-wzx-wcnU-wzy-wcnV-wcnW

Species

Escherichia coli/562

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

wcnP - ADN43876.1 107 - 1106 (+) GU068045.1:214-1213 -
wcnQ - ADN43877.1 1105 - 2260 (+) GU068045.1:1212-2367 -
wcnR - ADN43878.1 2256 - 2967 (+) GU068045.1:2363-3074 -
wcnS - ADN43879.1 2944 - 4483 (+) GU068045.1:3051-4590 -
wcnT - ADN43880.1 4472 - 5537 (+) GU068045.1:4579-5644 -
wzx - ADN43881.1 5533 - 6784 (+) GU068045.1:5640-6891 -
wcnU - ADN43882.1 6780 - 8214 (+) GU068045.1:6887-8321 -
wzy - ADN43883.1 8206 - 9544 (+) GU068045.1:8313-9651 -
wcnV - ADN43884.1 9536 - 10421 (+) GU068045.1:9643-10528 -
wcnW - ADN43885.1 10430 - 11174 (+) GU068045.1:10537-11281 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 108 - 1106 (+) TC: gnl|TC-DB|Q6MMD5|9.B.18.2.1 Yes
- 1106 - 2260 (+) STP: STP|Aminotran_1_2 Yes
- 2257 - 2967 (+) other Yes
- 2945 - 4483 (+) CAZyme: GT0 Yes
- 4473 - 5537 (+) other Yes
- 5534 - 6784 (+) TC: gnl|TC-DB|H2B831|2.A.66.2.19 Yes
- 6781 - 8214 (+) other Yes
- 8207 - 9544 (+) other Yes
- 9537 - 10421 (+) other Yes
- 10431 - 11174 (+) CAZyme: GT2|GT2_Glycos_transf_2 Yes

PUL ID

PUL0188

PubMed

26582605, Glycobiology. 2016 Apr;26(4):335-42. doi: 10.1093/glycob/cwv106. Epub 2015 Nov 17.

Title

Structure and gene cluster of the O-antigen of Escherichia coli O165 containing 5-N-acetyl-7-N-[(R)-3-hydroxybutanoyl]pseudaminic acid.

Author

Senchenkova SN, Zhang Y, Perepelov AV, Guo X, Shashkov AS, Weintraub A, Liu B, Widmalm G, Knirel YA

Abstract

Upon mild acid degradation of the lipopolysaccharide of Escherichia coli O165, the O-polysaccharide chain was cleaved at the glycosidic linkage of 5-N-acetyl-7-N-[(R)-3-hydroxybutanoyl]pseudaminic acid (Pse5Hb7Ac). Analysis of the resulting linear tetrasaccharide and alkali-treated lipopolysaccharide by (1)H/(13)C 1D and 2D nuclear magnetic resonance spectroscopy enabled elucidation of the following structure of the O-polysaccharide: -->8)-alpha-Psep5Hb7Ac-(2 --> 6)-beta-d-Galp-(1 --> 4)-beta-d-Glsmall es, Cyrillicp-(1 --> 3)-alpha-d-Glsmall es, CyrillicpNAc-(1-->. The beta-d-Galp-(1 --> 4)-beta-d-Glsmall es, Cyrillicp-(1 --> 3)-d-Glsmall es, CyrillicpNAc structural element is also present in the O-polysaccharide of E. coli O82. The content of the O-antigen gene cluster of E. coli O165 was found to be consistent with the O-polysaccharide structure established. Functions of proteins encoded in the gene cluster, including enzymes involved in the Pse5Hb7Ac biosynthesis and glycosyltransferases, were putatively assigned by comparison with sequences in available databases.