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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0204

PubMed

25841008, Appl Environ Microbiol. 2015 Jun 15;81(12):3973-83. doi: 10.1128/AEM.00149-15. Epub 2015 Apr 3.

Characterization method

qPCR,thin layer chromatography,substrate binding assay

Genomic accession number

NC_004663.1

Nucelotide position range

4800873-4814851

Substrate

exopolysaccharide

Loci

BT_3698-BT_3704

Species

Bacteroides thetaiotaomicron/818

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BT_RS18645 WP_011108937.1 0 - 2079 (-) NC_004663.1:4800873-4802952 -
- BT_RS18650 WP_008767007.1 2188 - 3646 (-) NC_004663.1:4803061-4804519 -
- BT_RS18655 WP_008767006.1 3671 - 4835 (-) NC_004663.1:4804544-4805708 -
susD BT_RS18660 WP_008767005.1 4869 - 6525 (-) NC_004663.1:4805742-4807398 -
- BT_RS18665 WP_011108938.1 6546 - 9558 (-) NC_004663.1:4807419-4810431 -
- BT_RS18670 WP_008767003.1 9712 - 11929 (-) NC_004663.1:4810585-4812802 -
- BT_RS18675 WP_008767002.1 12125 - 13979 (-) NC_004663.1:4812998-4814852 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 2079 (-) TC: gnl|TC-DB|Q05839|8.A.9.1.1 Yes
- 2189 - 3646 (-) other Yes
- 3672 - 4835 (-) other Yes
susD 4870 - 6525 (-) TC: gnl|TC-DB|Q8A1G2|8.A.46.1.1 Yes
- 6547 - 9558 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 9713 - 11929 (-) CAZyme: GH97 Yes
- 12126 - 13979 (-) TC: gnl|TC-DB|Q07837|8.A.9.1.2 Yes

PUL ID

PUL0204

PubMed

25841008, Appl Environ Microbiol. 2015 Jun 15;81(12):3973-83. doi: 10.1128/AEM.00149-15. Epub 2015 Apr 3.

Title

Differential Metabolism of Exopolysaccharides from Probiotic Lactobacilli by the Human Gut Symbiont Bacteroides thetaiotaomicron.

Author

Lammerts van Bueren A, Saraf A, Martens EC, Dijkhuizen L

Abstract

Probiotic microorganisms are ingested as food or supplements and impart positive health benefits to consumers. Previous studies have indicated that probiotics transiently reside in the gastrointestinal tract and, in addition to modulating commensal species diversity, increase the expression of genes for carbohydrate metabolism in resident commensal bacterial species. In this study, it is demonstrated that the human gut commensal species Bacteroides thetaiotaomicron efficiently metabolizes fructan exopolysaccharide (EPS) synthesized by probiotic Lactobacillus reuteri strain 121 while only partially degrading reuteran and isomalto/malto-polysaccharide (IMMP) alpha-glucan EPS polymers. B. thetaiotaomicron metabolized these EPS molecules via the activation of enzymes and transport systems encoded by dedicated polysaccharide utilization loci specific for beta-fructans and alpha-glucans. Reduced metabolism of reuteran and IMMP alpha-glucan EPS molecules may be due to reduced substrate binding by components of the starch utilization system (sus). This study reveals that microbial EPS substrates activate genes for carbohydrate metabolism in B. thetaiotaomicron and suggests that microbially derived carbohydrates provide a carbohydrate-rich reservoir for B. thetaiotaomicron nutrient acquisition in the gastrointestinal tract.