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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0271

PubMed

23663691, BMC Genomics. 2013 May 10;14:312. doi: 10.1186/1471-2164-14-312.

Characterization method

RT-qPCR

Genomic accession number

CP001515.1

Nucelotide position range

53714-59731

Substrate

gentiobiose

Loci

balac_0052-balac_0055

Species

Bifidobacterium animalis subsp. lactis/302911

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- Balac_0052 ACS45448.1 0 - 1527 (+) CP001515.1:53714-55241 -
- Balac_0053 ACS45449.1 1652 - 3758 (-) CP001515.1:55366-57472 -
- Balac_0054 ACS45450.1 4167 - 5322 (+) CP001515.1:57881-59036 -
- Balac_0055 ACS45451.1 5376 - 6018 (-) CP001515.1:59090-59732 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1527 (+) CAZyme: GH30_1 Yes
- 1653 - 3758 (-) CAZyme: GH42 Yes
- 4168 - 5322 (+) TC: gnl|TC-DB|J9W1F3|2.A.1.2.93 Yes
- 5377 - 6018 (-) STP: STP|TetR_N No

PUL ID

PUL0271

PubMed

23663691, BMC Genomics. 2013 May 10;14:312. doi: 10.1186/1471-2164-14-312.

Title

Transcriptional analysis of oligosaccharide utilization by Bifidobacterium lactis Bl-04.

Author

Andersen JM, Barrangou R, Abou Hachem M, Lahtinen SJ, Goh YJ, Svensson B, Klaenhammer TR

Abstract

BACKGROUND: Probiotic bifidobacteria in combination with prebiotic carbohydrates have documented positive effects on human health regarding gastrointestinal disorders and improved immunity, however the selective routes of uptake remain unknown for most candidate prebiotics. The differential transcriptomes of Bifidobacterium animalis subsp. lactis Bl-04, induced by 11 potential prebiotic oligosaccharides were analyzed to identify the genetic loci involved in the uptake and catabolism of alpha- and beta-linked hexoses, and beta-xylosides. RESULTS: The overall transcriptome was modulated dependent on the type of glycoside (galactosides, glucosides or xylosides) utilized. Carbohydrate transporters of the major facilitator superfamily (induced by gentiobiose and beta-galacto-oligosaccharides (GOS)) and ATP-binding cassette (ABC) transporters (upregulated by cellobiose, GOS, isomaltose, maltotriose, melibiose, panose, raffinose, stachyose, xylobiose and beta-xylo-oligosaccharides) were differentially upregulated, together with glycoside hydrolases from families 1, 2, 13, 36, 42, 43 and 77. Sequence analysis of the identified solute-binding proteins that determine the specificity of ABC transporters revealed similarities in the breadth and selectivity of prebiotic utilization by bifidobacteria. CONCLUSION: This study identified the differential gene expression for utilization of potential prebiotics highlighting the extensive capabilities of Bifidobacterium lactis Bl-04 to utilize oligosaccharides. Results provide insights into the ability of this probiotic microbe to utilize indigestible carbohydrates in the human gastrointestinal tract.