PUL ID

PUL0293

PubMed

29360976, FEMS Microbiol Lett. 2018 Feb 1;365(4). pii: 4816728. doi: 10.1093/femsle/fnx280.

Characterization method

RT-qPCR, gene deletion mutant and growth assay

Genomic accession number

KU644713.1

Nucelotide position range

1-16598

Substrate

mannan

Loci

manD- manR- manE- manH- manK- manC- manI- manJ- manB- manG- manF- manA+

Species

Bacillus sp. N16-5/122631

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- - AML27054.1 0 - 1020 (-) KU644713.1:1-1021 -
- - AML27055.1 1299 - 2304 (-) KU644713.1:1300-2305 -
- - AML27056.1 2365 - 3541 (-) KU644713.1:2366-3542 -
- - AML27057.1 3776 - 5084 (-) KU644713.1:3777-5085 -
- - AML27058.1 5854 - 7060 (-) KU644713.1:5855-7061 -
- - AML27059.1 8026 - 9196 (-) KU644713.1:8027-9197 -
- - AML27060.1 9549 - 10353 (-) KU644713.1:9550-10354 -
- - AML27061.1 10355 - 11297 (-) KU644713.1:10356-11298 -
- - AML27062.1 11363 - 12308 (-) KU644713.1:11364-12309 -
- - AML27063.1 12329 - 13544 (-) KU644713.1:12330-13545 -
- - AML27064.1 13791 - 14748 (-) KU644713.1:13792-14749 -
- - AML27065.1 15116 - 16598 (+) KU644713.1:15117-16599 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1020 (-) CAZyme: GH130 Yes
- 1300 - 2304 (-) TF: DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955 Yes
- 2366 - 3541 (-) CAZyme: GH130 Yes
- 3777 - 5084 (-) STP: STP|SBP_bac_1 Yes
- 5855 - 7060 (-) other Yes
- 8027 - 9196 (-) CAZyme: GH27 Yes
- 9550 - 10353 (-) TC: gnl|TC-DB|Q8DT26|3.A.1.1.27 Yes
- 10356 - 11297 (-) TC: gnl|TC-DB|P94529|3.A.1.1.34 Yes
- 11364 - 12308 (-) other Yes
- 12330 - 13544 (-) other Yes
- 13792 - 14748 (-) CAZyme: CE7 Yes
- 15117 - 16598 (+) CDS No

PUL ID

PUL0293

PubMed

29360976, FEMS Microbiol Lett. 2018 Feb 1;365(4). pii: 4816728. doi: 10.1093/femsle/fnx280.

Title

Transcriptional regulation of the mannan utilization genes in the alkaliphilic Bacillus sp. N16-5.

Author

Song Y, Liu D, Liu M, Yang H, Fan Y, Sun W, Xue Y, Zhang T, Ma Y

Abstract

Bacillus sp. N16-5 is an alkaliphile with a great ability to utilize mannan. Its mannan utilization gene cluster has been identified in a previous study. The ManR protein encoded by the cluster was predicted to be a LacI family regulator, and the transcription level of the mannan utilization gene cluster was upregulated after the manR gene was deleted, indicating that ManR is the repressor of this cluster. The transcription of the related genes was downregulated when manH, encoding the extracellular substrate-binding domain of the manno-oligosaccharide transporter, was deleted. Furthermore, isothermal titration calorimetry revealed that mannotetraose and mannopentose are ligands of ManR. These results all corroborate the hypothesis that the mannan utilization gene cluster is repressed by the transcription regulator ManR, and that the repression is removed when it binds to manno-oligosaccharides, which are generated by mannan degradation and transported into the cell by a specific transporter.