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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0350

PubMed

16968696, J Biol Chem. 2006 Nov 24;281(47):36269-79. doi: 10.1074/jbc.M606509200. Epub 2006 Sep 12.

Characterization method

microarray

Genomic accession number

AE015928.1

Nucelotide position range

1663708-1685198

Substrate

capsule polysaccharide

Loci

BT_1338-BT_1358

Species

Bacteroides thetaiotaomicron/818

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BT_1338 AAO76445.1 0 - 549 (-) AE015928.1:1663708-1664257 -
- BT_1339 AAO76446.1 572 - 1679 (-) AE015928.1:1664280-1665387 -
- BT_1340 AAO76447.1 1696 - 2509 (-) AE015928.1:1665404-1666217 -
- BT_1341 AAO76448.1 2534 - 3848 (-) AE015928.1:1666242-1667556 -
- BT_1342 AAO76449.1 3855 - 4917 (-) AE015928.1:1667563-1668625 -
- BT_1343 AAO76450.1 4945 - 6013 (-) AE015928.1:1668653-1669721 -
- BT_1344 AAO76451.1 6009 - 7113 (-) AE015928.1:1669717-1670821 -
- BT_1345 AAO76452.1 7112 - 8006 (-) AE015928.1:1670820-1671714 -
- BT_1346 AAO76453.1 8023 - 9049 (-) AE015928.1:1671731-1672757 -
- BT_1347 AAO76454.1 9057 - 9915 (-) AE015928.1:1672765-1673623 -
- BT_1348 AAO76455.1 9940 - 10831 (-) AE015928.1:1673648-1674539 -
- BT_1349 AAO76456.1 10827 - 11274 (-) AE015928.1:1674535-1674982 -
- BT_1350 AAO76457.1 11261 - 12362 (-) AE015928.1:1674969-1676070 -
- BT_1351 AAO76458.1 12373 - 13147 (-) AE015928.1:1676081-1676855 -
- BT_1352 AAO76459.1 13148 - 14318 (-) AE015928.1:1676856-1678026 -
- BT_1353 AAO76460.1 14331 - 15369 (-) AE015928.1:1678039-1679077 -
- BT_1354 AAO76461.1 15368 - 16910 (-) AE015928.1:1679076-1680618 -
- BT_1355 AAO76462.1 16923 - 18063 (-) AE015928.1:1680631-1681771 -
- BT_1356 AAO76463.1 18077 - 20447 (-) AE015928.1:1681785-1684155 -
- BT_1357 AAO76464.1 20504 - 20873 (-) AE015928.1:1684212-1684581 -
- BT_1358 AAO76465.1 20918 - 21491 (-) AE015928.1:1684626-1685199 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 549 (-) CDS No
- 573 - 1679 (-) TC: gnl|TC-DB|R6S968|9.B.146.1.4 Yes
- 1697 - 2509 (-) other Yes
- 2535 - 3848 (-) STP: STP|Pyr_redox Yes
- 3856 - 4917 (-) other Yes
- 4946 - 6013 (-) other Yes
- 6010 - 7113 (-) CAZyme: GT4 Yes
- 7113 - 8006 (-) other Yes
- 8024 - 9049 (-) other Yes
- 9058 - 9915 (-) CAZyme: GT2_Glycos_transf_2|GT2 Yes
- 9941 - 10831 (-) other Yes
- 10828 - 11274 (-) other Yes
- 11262 - 12362 (-) other Yes
- 12374 - 13147 (-) other Yes
- 13149 - 14318 (-) CAZyme: GT4 Yes
- 14332 - 15369 (-) CAZyme: GT2_Glycos_transf_2|GT2 Yes
- 15369 - 16910 (-) CDS No
- 16924 - 18063 (-) CDS No
- 18078 - 20447 (-) CDS No
- 20505 - 20873 (-) CDS No
- 20919 - 21491 (-) CDS No

PUL ID

PUL0350

PubMed

16968696, J Biol Chem. 2006 Nov 24;281(47):36269-79. doi: 10.1074/jbc.M606509200. Epub 2006 Sep 12.

Title

Functional genomic and metabolic studies of the adaptations of a prominent adult human gut symbiont, Bacteroides thetaiotaomicron, to the suckling period.

Author

Bjursell MK, Martens EC, Gordon JI

Abstract

The adult human gut microbiota is dominated by two divisions of Bacteria, the Bacteroidetes and the Firmicutes. Assembly of this community begins at birth through processes that remain largely undefined. In this report, we examine the adaptations of Bacteroides thetaiotaomicron, a prominent member of the adult distal intestinal microbiota, during the suckling and weaning periods. Germ-free NMRI mice were colonized at birth from their gnotobiotic mothers, who harbored this anaerobic Gram-negative saccharolytic bacterium. B. thetaiotaomicron was then harvested from the ceca of these hosts during the suckling period (postnatal day 17) and after weaning (postnatal day 30). Whole genome transcriptional profiles were obtained at these two time points using custom B. thetaiotaomicron GeneChips. Transcriptome-based in silico reconstructions of bacterial metabolism and gas chromatography-mass spectrometry and biochemical assays of carbohydrate utilization in vivo indicated that in the suckling gut B. thetaiotaomicron prefers host-derived polysaccharides, as well as mono- and oligosaccharides present in mother's milk. After weaning, B. thetaiotaomicron expands its metabolism to exploit abundant, plant-derived dietary polysaccharides. The bacterium's responses to postnatal alterations in its nutrient landscape involve expression of gene clusters encoding environmental sensors, outer membrane proteins involved in binding and import of glycans, and glycoside hydrolases. These expression changes are interpreted in light of a phylogenetic analysis that revealed unique expansions of related polysaccharide utilization loci in three human alimentary tract-associated Bacteroidetes, expansions that likely reflect the evolutionary adaptations of these species to different nutrient niches.