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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0384

PubMed

18996345, Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.

Characterization method

microarray,qPCR

Genomic accession number

AE015928.1

Nucelotide position range

6085860-6111470

Substrate

glycosaminoglycan

Loci

BT_4652-BT_4663

Species

Bacteroides thetaiotaomicron/818

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BT_4652 AAO79757.1 0 - 2619 (-) AE015928.1:6085860-6088479 -
- BT_4653 AAO79758.1 2623 - 3871 (-) AE015928.1:6088483-6089731 -
- BT_4654 AAO79759.1 3926 - 4880 (-) AE015928.1:6089786-6090740 -
- BT_4655 AAO79760.1 5040 - 6558 (-) AE015928.1:6090900-6092418 -
- BT_4656 AAO79761.1 6690 - 8367 (-) AE015928.1:6092550-6094227 -
- BT_4657 AAO79762.1 8412 - 10413 (-) AE015928.1:6094272-6096273 -
- BT_4658 AAO79763.1 10468 - 11773 (-) AE015928.1:6096328-6097633 -
- BT_4659 AAO79764.1 11957 - 13631 (-) AE015928.1:6097817-6099491 -
- BT_4660 AAO79765.1 13648 - 16792 (-) AE015928.1:6099508-6102652 -
- BT_4661 AAO79766.1 16816 - 18997 (-) AE015928.1:6102676-6104857 -
- BT_4662 AAO79767.1 19015 - 21124 (-) AE015928.1:6104875-6106984 -
- BT_4663 AAO79768.1 21549 - 25611 (-) AE015928.1:6107409-6111471 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 2619 (-) CAZyme: PL15_2|PL15 Yes
- 2624 - 3871 (-) TC: gnl|TC-DB|Q89YS8|2.A.1.7.18 Yes
- 3927 - 4880 (-) other Yes
- 5041 - 6558 (-) other Yes
- 6691 - 8367 (-) other Yes
- 8413 - 10413 (-) CAZyme: PL12|PL12_2 Yes
- 10469 - 11773 (-) CAZyme: GH88 Yes
- 11958 - 13631 (-) TC: gnl|TC-DB|C6Y217|8.A.46.1.3 Yes
- 13649 - 16792 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 16817 - 18997 (-) other Yes
- 19016 - 21124 (-) CAZyme: PL12_2 Yes
- 21550 - 25611 (-) TF: DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0035607 No

PUL ID

PUL0384

PubMed

18996345, Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.

Title

Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont.

Author

Martens EC, Chiang HC, Gordon JI

Abstract

The distal human gut is a microbial bioreactor that digests complex carbohydrates. The strategies evolved by gut microbes to sense and process diverse glycans have important implications for the assembly and operation of this ecosystem. The human gut-derived bacterium Bacteroides thetaiotaomicron forages on both host and dietary glycans. Its ability to target these substrates resides in 88 polysaccharide utilization loci (PULs), encompassing 18% of its genome. Whole genome transcriptional profiling and genetic tests were used to define the mechanisms underlying host glycan foraging in vivo and in vitro. PULs that target all major classes of host glycans were identified. However, mucin O-glycans are the principal host substrate foraged in vivo. Simultaneous deletion of five genes encoding ECF-sigma transcription factors, which activate mucin O-glycan utilization, produces defects in bacterial persistence in the gut and in mother-to-offspring transmission. Thus, PUL-mediated glycan catabolism is an important component in gut colonization and may impact microbiota ecology.