Home Repository Download Help About Statistics Taxonomy Blastx
Links
PULDB
CAZy
dbCAN2
dbCAN-seq
run_dbcan
CAZypedia
PlantCAZyme
Cite: NAR/gkaa742 2020

Because CGCFinder predicted no CGC for this PUL, the gene cluster depicted below contains dbCAN2 and CGC signature predictions for all genes in the PUL, instead of a predicted CGC.


PUL General Information

Literature Information

PUL ID

PUL0388

PubMed

20501518, Innate Immun. 2011 Apr;17(2):164-73. doi: 10.1177/1753425910369270. Epub 2010 May 25.

Characterization method

sugar utilization assay,NMR

Genomic accession number

LS483455.1

Nucelotide position range

1712292-1722235

Substrate

O-antigen

Loci

None

Species

Salmonella enterica/28901

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

galF NCTC9872_01630 SQH84227.1 0 - 765 (+) LS483455.1:1712292-1713057 2.7.7.9
wecB_1 NCTC9872_01632 SQH84228.1 1108 - 2245 (+) LS483455.1:1713400-1714537 5.1.3.14
- NCTC9872_01633 SQH84229.1 2277 - 3396 (+) LS483455.1:1714569-1715688 -
tagE_2 NCTC9872_01634 SQH84230.1 3370 - 4417 (+) LS483455.1:1715662-1716709 2.4.1.52
- NCTC9872_01635 SQH84231.1 4431 - 5652 (+) LS483455.1:1716723-1717944 -
- NCTC9872_01636 SQH84232.1 5641 - 6799 (+) LS483455.1:1717933-1719091 2.4.1.11
- NCTC9872_01637 SQH84233.1 6801 - 7914 (+) LS483455.1:1719093-1720206 -
glf NCTC9872_01638 SQH84234.1 7918 - 9016 (+) LS483455.1:1720210-1721308 5.4.99.9
glfT1 NCTC9872_01639 SQH84235.1 9015 - 9933 (+) LS483455.1:1721307-1722225 2.4.1.-

Cluster number

0

Gene name

Gene position

Gene type

Found by CGCFinder?

galF 1 - 765 (+) CDS No
wecB_1 1109 - 2245 (+) CDS No
- 2278 - 3396 (+) CDS No
tagE_2 3371 - 4417 (+) CAZyme: GT4 No
- 4432 - 5652 (+) CDS No
- 5642 - 6799 (+) CAZyme: GT4 No
- 6802 - 7914 (+) CDS No
glf 7919 - 9016 (+) STP: STP|Pyr_redox No
glfT1 9016 - 9933 (+) CAZyme: GT2|GT2_Glycos_transf_2 No

PUL ID

PUL0388

PubMed

20501518, Innate Immun. 2011 Apr;17(2):164-73. doi: 10.1177/1753425910369270. Epub 2010 May 25.

Title

Structural and genetic characterization of the closely related O-antigens of Escherichia coli O85 and Salmonella enterica O17.

Author

Perepelov AV, Li D, Liu B, Senchenkova SN, Guo D, Shashkov AS, Feng L, Knirel YA, Wang L

Abstract

O-Antigen is a part of the lipopolysaccharide present in the outer membrane of Gram-negative bacteria, which confers major antigenic variability to the cell surface. In this study, we report on a previously undefined pair of Escherichia coli and Salmonella enterica with closely related O-antigens. The O-polysaccharides were isolated from the lipopolysaccharides of E. coli O85 and S. enterica O17 by mild acid degradation and studied by sugar analysis and NMR spectroscopy. The following structure was established for the O-unit of the E. coli O85-polysaccharide: The S. enterica O17-polysaccharide has the same carbohydrate backbone and, in addition, contains an O-acetyl group at position 2 of ~80% beta-Galf residues. The O-antigen gene cluster of E. coli O85 was found to be closely related to that of S. enterica O17. Screening of type strains of all E. coli and S. enterica O-serogroups revealed two genes specific to the E. coli O85 O-antigen gene cluster, which can be used for development of PCR-based assays for identification and detection of E. coli O85 strains.