Because CGCFinder predicted no CGC for this PUL, the gene cluster depicted below contains dbCAN2 and CGC signature predictions for all genes in the PUL, instead of a predicted CGC.


PUL ID

PUL0389

PubMed

20501518, Innate Immun. 2011 Apr;17(2):164-73. doi: 10.1177/1753425910369270. Epub 2010 May 25.

Characterization method

sugar utilization assay, NMR

Genomic accession number

GU299798

Nucelotide position range

1102-9919

Substrate

O-antigen

Loci

None

Species

Escherichia coli/562

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

mnaA - ADC54973.1 1102 - 2239 (+) GU299798.1:2204-3341 -
wzy - ADC54974.1 2265 - 3384 (+) GU299798.1:3367-4486 -
wfbQ - ADC54975.1 3358 - 4411 (+) GU299798.1:4460-5513 -
wzx - ADC54976.1 4419 - 5640 (+) GU299798.1:5521-6742 -
wfbR - ADC54977.1 5629 - 6796 (+) GU299798.1:6731-7898 -
wfbS - ADC54978.1 6792 - 7908 (+) GU299798.1:7894-9010 -
glf - ADC54979.1 7907 - 9008 (+) GU299798.1:9009-10110 -
wfbU - ADC54980.1 9004 - 9919 (+) GU299798.1:10106-11021 -

Cluster number

0

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1103 - 2239 (+) CDS No
- 2266 - 3384 (+) CDS No
- 3359 - 4411 (+) CAZyme: GT4 No
- 4420 - 5640 (+) CDS No
- 5630 - 6796 (+) CAZyme: GT4 No
- 6793 - 7908 (+) CDS No
- 7908 - 9008 (+) CDS No
- 9005 - 9919 (+) CAZyme: GT2_Glycos_transf_2|GT2 No

PUL ID

PUL0389

PubMed

20501518, Innate Immun. 2011 Apr;17(2):164-73. doi: 10.1177/1753425910369270. Epub 2010 May 25.

Title

Structural and genetic characterization of the closely related O-antigens of Escherichia coli O85 and Salmonella enterica O17.

Author

Perepelov AV, Li D, Liu B, Senchenkova SN, Guo D, Shashkov AS, Feng L, Knirel YA, Wang L

Abstract

O-Antigen is a part of the lipopolysaccharide present in the outer membrane of Gram-negative bacteria, which confers major antigenic variability to the cell surface. In this study, we report on a previously undefined pair of Escherichia coli and Salmonella enterica with closely related O-antigens. The O-polysaccharides were isolated from the lipopolysaccharides of E. coli O85 and S. enterica O17 by mild acid degradation and studied by sugar analysis and NMR spectroscopy. The following structure was established for the O-unit of the E. coli O85-polysaccharide: The S. enterica O17-polysaccharide has the same carbohydrate backbone and, in addition, contains an O-acetyl group at position 2 of ~80% beta-Galf residues. The O-antigen gene cluster of E. coli O85 was found to be closely related to that of S. enterica O17. Screening of type strains of all E. coli and S. enterica O-serogroups revealed two genes specific to the E. coli O85 O-antigen gene cluster, which can be used for development of PCR-based assays for identification and detection of E. coli O85 strains.