PUL ID

PUL0452

PubMed

28837839, Carbohydr Res. 2017 Oct 10;450:19-29. doi: 10.1016/j.carres.2017.08.006. Epub 2017 Aug 18.

Characterization method

sequence homology analysis,sugar utilization assay,NMR,gas chromatography and mass spectrometry

Genomic accession number

CR931684.1

Nucelotide position range

2578-21228

Substrate

O-antigen

Loci

SPC23B_0001-SPC23B_0023

Species

Streptococcus pneumoniae/1313

Degradation or Biosynthesis

biosynthesis

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

wzg 2578 - 4032 (+) CDS No
wzh 3986 - 4765 (+) CDS No
wzd 4774 - 5466 (+) TC: gnl|TC-DB|Q93E03|8.A.3.2.3 Yes
wze 5476 - 6165 (+) TC: gnl|TC-DB|Q93NW3|8.A.3.2.2 Yes
wchA 6180 - 7541 (+) TC: gnl|TC-DB|H8E4X1|9.B.18.1.2 Yes
wchF 7528 - 8751 (+) CAZyme: GT4 Yes
wzy 9052 - 10239 (+) other Yes
wchV 10249 - 11217 (+) CAZyme: GT2|GT2_Glycos_transf_2 Yes
wchW 11224 - 12462 (+) CDS No
wzx 12455 - 13843 (+) CDS No
wchX 13840 - 14994 (+) CDS No
gtp1 15030 - 16058 (+) CDS No
gtp2 16074 - 16778 (+) CDS No
gtp3 16775 - 17626 (+) CDS No
rmlA 17786 - 18655 (+) CDS No
rmlC 18656 - 19249 (+) CDS No
rmlB 19262 - 20311 (+) CDS No
rmlD 20377 - 21228 (+) CDS No

PUL ID

PUL0452

PubMed

28837839, Carbohydr Res. 2017 Oct 10;450:19-29. doi: 10.1016/j.carres.2017.08.006. Epub 2017 Aug 18.

Title

Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F.

Author

Ravenscroft N, Omar A, Hlozek J, Edmonds-Smith C, Follador R, Serventi F, Lipowsky G, Kuttel MM, Cescutti P, Faridmoayer A

Abstract

Streptococcus pneumoniae is a globally important encapsulated human pathogen with approximately 100 different serotypes recognized. Serogroup 23 consists of serotype 23F, present in licensed vaccines, and emerging serotypes 23A and 23B. Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A). The structure of the serotype 23A capsular polysaccharide is: -->4)-beta-D-Glcp-(1-->3)-[[alpha-L-Rhap-(1-->2)]-[Gro-(2-->P-->3)]-beta-D-Galp-(1-->4)]-beta-L-Rhap-(1-->. This structure differs from polysaccharide 23F as it features a disaccharide backbone and the di-substituted beta-Gal is linked to beta-Rha as a side chain. This is due to the different polymerization position catalysed by the unusually divergent repeat unit polymerase Wzy in the 23A cps biosynthesis locus. Steric crowding in 23A, confirmed by molecular models, causes the NMR signal for H-1 of the di-substituted 2,3-beta-Gal to resonate in the alpha-anomeric region. The structure of the serotype 23B capsular polysaccharide is the same as 23F, but without the terminal alpha-Rha: -->4)-beta-D-Glcp-(1-->4)-[Gro-(2-->P-->3)]-beta-D-Galp-(1-->4)-beta-L-Rhap-(1-->. The immunodominant terminal alpha-Rha of 23F is more sterically crowded in 23A and absent in 23B. This may explain the reported typing cross reactions for serotype 23F: slight with 23A and none with 23B.