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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0452

PubMed

28837839, Carbohydr Res. 2017 Oct 10;450:19-29. doi: 10.1016/j.carres.2017.08.006. Epub 2017 Aug 18.

Characterization method

sequence homology analysis,sugar utilization assay,NMR,gas chromatography and mass spectrometry

Genomic accession number

CR931684.1

Nucelotide position range

2578-21228

Substrate

O-antigen

Loci

SPC23B_0001-SPC23B_0023

Species

Streptococcus pneumoniae/1313

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

wzg SPC23B_0004 CAI33834.1 2577 - 4032 (+) CR931684.1:5155-6610 -
wzh SPC23B_0005 CAI33835.1 3985 - 4765 (+) CR931684.1:6563-7343 3.1.3.48
wzd SPC23B_0006 CAI33836.1 4773 - 5466 (+) CR931684.1:7351-8044 -
wze SPC23B_0007 CAI33837.1 5475 - 6165 (+) CR931684.1:8053-8743 2.7.10.1
wchA SPC23B_0008 CAI33838.1 6179 - 7541 (+) CR931684.1:8757-10119 -
wchF SPC23B_0009 CAI33839.1 7527 - 8751 (+) CR931684.1:10105-11329 -
wzy SPC23B_0010 CAI33840.1 9051 - 10239 (+) CR931684.1:11629-12817 -
wchV SPC23B_0011 CAI33841.1 10248 - 11217 (+) CR931684.1:12826-13795 -
wchW SPC23B_0012 CAI33842.1 11223 - 12462 (+) CR931684.1:13801-15040 -
wzx SPC23B_0013 CAI33843.1 12454 - 13843 (+) CR931684.1:15032-16421 -
wchX SPC23B_0014 CAI33844.1 13839 - 14994 (+) CR931684.1:16417-17572 -
gtp1 SPC23B_0015 CAI33845.1 15029 - 16058 (+) CR931684.1:17607-18636 -
gtp2 SPC23B_0016 CAI33846.1 16073 - 16778 (+) CR931684.1:18651-19356 -
gtp3 SPC23B_0017 CAI33847.1 16774 - 17626 (+) CR931684.1:19352-20204 -
rmlA SPC23B_0018 CAI33848.1 17785 - 18655 (+) CR931684.1:20363-21233 2.7.7.24
rmlC SPC23B_0019 CAI33849.1 18655 - 19249 (+) CR931684.1:21233-21827 5.1.3.13
rmlB SPC23B_0020 CAI33850.1 19261 - 20311 (+) CR931684.1:21839-22889 4.2.1.46
rmlD SPC23B_0021 CAI33851.1 20376 - 21228 (+) CR931684.1:22954-23806 1.1.1.133

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

wzg 2578 - 4032 (+) CDS No
wzh 3986 - 4765 (+) CDS No
wzd 4774 - 5466 (+) TC: gnl|TC-DB|Q93E03|8.A.3.2.3 Yes
wze 5476 - 6165 (+) TC: gnl|TC-DB|Q93NW3|8.A.3.2.2 Yes
wchA 6180 - 7541 (+) TC: gnl|TC-DB|H8E4X1|9.B.18.1.2 Yes
wchF 7528 - 8751 (+) CAZyme: GT4 Yes
wzy 9052 - 10239 (+) other Yes
wchV 10249 - 11217 (+) CAZyme: GT2|GT2_Glycos_transf_2 Yes
wchW 11224 - 12462 (+) CDS No
wzx 12455 - 13843 (+) CDS No
wchX 13840 - 14994 (+) CDS No
gtp1 15030 - 16058 (+) CDS No
gtp2 16074 - 16778 (+) CDS No
gtp3 16775 - 17626 (+) CDS No
rmlA 17786 - 18655 (+) CDS No
rmlC 18656 - 19249 (+) CDS No
rmlB 19262 - 20311 (+) CDS No
rmlD 20377 - 21228 (+) CDS No

PUL ID

PUL0452

PubMed

28837839, Carbohydr Res. 2017 Oct 10;450:19-29. doi: 10.1016/j.carres.2017.08.006. Epub 2017 Aug 18.

Title

Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F.

Author

Ravenscroft N, Omar A, Hlozek J, Edmonds-Smith C, Follador R, Serventi F, Lipowsky G, Kuttel MM, Cescutti P, Faridmoayer A

Abstract

Streptococcus pneumoniae is a globally important encapsulated human pathogen with approximately 100 different serotypes recognized. Serogroup 23 consists of serotype 23F, present in licensed vaccines, and emerging serotypes 23A and 23B. Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A). The structure of the serotype 23A capsular polysaccharide is: -->4)-beta-D-Glcp-(1-->3)-[[alpha-L-Rhap-(1-->2)]-[Gro-(2-->P-->3)]-beta-D-Galp-(1-->4)]-beta-L-Rhap-(1-->. This structure differs from polysaccharide 23F as it features a disaccharide backbone and the di-substituted beta-Gal is linked to beta-Rha as a side chain. This is due to the different polymerization position catalysed by the unusually divergent repeat unit polymerase Wzy in the 23A cps biosynthesis locus. Steric crowding in 23A, confirmed by molecular models, causes the NMR signal for H-1 of the di-substituted 2,3-beta-Gal to resonate in the alpha-anomeric region. The structure of the serotype 23B capsular polysaccharide is the same as 23F, but without the terminal alpha-Rha: -->4)-beta-D-Glcp-(1-->4)-[Gro-(2-->P-->3)]-beta-D-Galp-(1-->4)-beta-L-Rhap-(1-->. The immunodominant terminal alpha-Rha of 23F is more sterically crowded in 23A and absent in 23B. This may explain the reported typing cross reactions for serotype 23F: slight with 23A and none with 23B.