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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0453

PubMed

28837839, Carbohydr Res. 2017 Oct 10;450:19-29. doi: 10.1016/j.carres.2017.08.006. Epub 2017 Aug 18.

Characterization method

sequence homology analysis,sugar utilization assay,NMR,gas chromatography and mass spectrometry

Genomic accession number

CR931685.1

Nucelotide position range

1881-20535

Substrate

O-antigen

Loci

SPC23F_0001-SPC23F_0022

Species

Streptococcus pneumoniae/1313

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

wzg SPC23F_0003 CAI33856.1 1881 - 3327 (+) CR931685.1:3762-5208 -
wzh SPC23F_0004 CAI33857.1 3328 - 4060 (+) CR931685.1:5209-5941 3.1.3.48
wzd SPC23F_0005 CAI33858.1 4065 - 4761 (+) CR931685.1:5946-6642 -
wze SPC23F_0006 CAI33859.1 4770 - 5460 (+) CR931685.1:6651-7341 2.7.10.1
wchA SPC23F_0007 CAI33860.1 5474 - 6842 (+) CR931685.1:7355-8723 -
wchF SPC23F_0008 CAI33861.1 6872 - 8051 (+) CR931685.1:8753-9932 -
wzy SPC23F_0009 CAI33862.1 8350 - 9544 (+) CR931685.1:10231-11425 -
wchV SPC23F_0010 CAI33863.1 9547 - 10519 (+) CR931685.1:11428-12400 -
wchW SPC23F_0011 CAI33864.1 10525 - 11758 (+) CR931685.1:12406-13639 -
wzx SPC23F_0012 CAI33865.1 11759 - 13145 (+) CR931685.1:13640-15026 -
wchX SPC23F_0013 CAI33866.1 13152 - 14301 (+) CR931685.1:15033-16182 -
gtp1 SPC23F_0014 CAI33867.1 14336 - 15365 (+) CR931685.1:16217-17246 -
gtp2 SPC23F_0015 CAI33868.1 15380 - 16085 (+) CR931685.1:17261-17966 -
gtp3 SPC23F_0016 CAI33869.1 16099 - 16933 (+) CR931685.1:17980-18814 -
rmlA SPC23F_0017 CAI33870.1 17092 - 17962 (+) CR931685.1:18973-19843 2.7.7.24
rmlC SPC23F_0018 CAI33871.1 17962 - 18556 (+) CR931685.1:19843-20437 5.1.3.13
rmlB SPC23F_0019 CAI33872.1 18568 - 19618 (+) CR931685.1:20449-21499 4.2.1.46
rmlD SPC23F_0020 CAI33873.1 19683 - 20535 (+) CR931685.1:21564-22416 1.1.1.133

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

wzg 1882 - 3327 (+) CDS No
wzh 3329 - 4060 (+) CDS No
wzd 4066 - 4761 (+) TC: gnl|TC-DB|Q93E03|8.A.3.2.3 Yes
wze 4771 - 5460 (+) TC: gnl|TC-DB|Q93NW3|8.A.3.2.2 Yes
wchA 5475 - 6842 (+) TC: gnl|TC-DB|H8E4X1|9.B.18.1.2 Yes
wchF 6873 - 8051 (+) CAZyme: GT4 Yes
wzy 8351 - 9544 (+) other Yes
wchV 9548 - 10519 (+) TC: gnl|TC-DB|P75905|4.D.1.1.3 Yes
wchW 10526 - 11758 (+) CDS No
wzx 11760 - 13145 (+) CDS No
wchX 13153 - 14301 (+) CDS No
gtp1 14337 - 15365 (+) CDS No
gtp2 15381 - 16085 (+) CDS No
gtp3 16100 - 16933 (+) CDS No
rmlA 17093 - 17962 (+) CDS No
rmlC 17963 - 18556 (+) CDS No
rmlB 18569 - 19618 (+) CDS No
rmlD 19684 - 20535 (+) CDS No

PUL ID

PUL0453

PubMed

28837839, Carbohydr Res. 2017 Oct 10;450:19-29. doi: 10.1016/j.carres.2017.08.006. Epub 2017 Aug 18.

Title

Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F.

Author

Ravenscroft N, Omar A, Hlozek J, Edmonds-Smith C, Follador R, Serventi F, Lipowsky G, Kuttel MM, Cescutti P, Faridmoayer A

Abstract

Streptococcus pneumoniae is a globally important encapsulated human pathogen with approximately 100 different serotypes recognized. Serogroup 23 consists of serotype 23F, present in licensed vaccines, and emerging serotypes 23A and 23B. Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A). The structure of the serotype 23A capsular polysaccharide is: -->4)-beta-D-Glcp-(1-->3)-[[alpha-L-Rhap-(1-->2)]-[Gro-(2-->P-->3)]-beta-D-Galp-(1-->4)]-beta-L-Rhap-(1-->. This structure differs from polysaccharide 23F as it features a disaccharide backbone and the di-substituted beta-Gal is linked to beta-Rha as a side chain. This is due to the different polymerization position catalysed by the unusually divergent repeat unit polymerase Wzy in the 23A cps biosynthesis locus. Steric crowding in 23A, confirmed by molecular models, causes the NMR signal for H-1 of the di-substituted 2,3-beta-Gal to resonate in the alpha-anomeric region. The structure of the serotype 23B capsular polysaccharide is the same as 23F, but without the terminal alpha-Rha: -->4)-beta-D-Glcp-(1-->4)-[Gro-(2-->P-->3)]-beta-D-Galp-(1-->4)-beta-L-Rhap-(1-->. The immunodominant terminal alpha-Rha of 23F is more sterically crowded in 23A and absent in 23B. This may explain the reported typing cross reactions for serotype 23F: slight with 23A and none with 23B.