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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0467

PubMed

18996345, Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.

Characterization method

microarray,qPCR

Genomic accession number

AE015928.1

Nucelotide position range

1998220-2027198

Substrate

host glycan

Loci

BT1623-BT1636

Species

Bacteroides thetaiotaomicron/818

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BT_1623 AAO76730.1 0 - 1176 (-) AE015928.1:1998220-1999396 -
- BT_1624 AAO76731.1 1310 - 2858 (-) AE015928.1:1999530-2001078 -
- BT_1625 AAO76732.1 2944 - 4762 (-) AE015928.1:2001164-2002982 -
- BT_1626 AAO76733.1 4929 - 7998 (-) AE015928.1:2003149-2006218 -
- BT_1627 AAO76734.1 8026 - 10357 (-) AE015928.1:2006246-2008577 -
- BT_1628 AAO76735.1 10372 - 12112 (-) AE015928.1:2008592-2010332 -
- BT_1629 AAO76736.1 12157 - 13939 (-) AE015928.1:2010377-2012159 -
- BT_1630 AAO76737.1 13984 - 15760 (-) AE015928.1:2012204-2013980 -
- BT_1631 AAO76738.1 15815 - 19175 (-) AE015928.1:2014035-2017395 -
- BT_1632 AAO76739.1 19205 - 20870 (-) AE015928.1:2017425-2019090 -
- BT_1633 AAO76740.1 21132 - 21876 (-) AE015928.1:2019352-2020096 -
- BT_1634 AAO76741.1 21884 - 23315 (-) AE015928.1:2020104-2021535 -
- BT_1635 AAO76742.1 23446 - 27412 (+) AE015928.1:2021666-2025632 -
- BT_1636 AAO76743.1 27449 - 28979 (+) AE015928.1:2025669-2027199 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1176 (-) TC: gnl|TC-DB|P0AAT4|1.A.23.4.5 Yes
- 1311 - 2858 (-) other Yes
- 2945 - 4762 (-) CAZyme: GH29|CBM32 Yes
- 4930 - 7998 (-) CAZyme: GH2 Yes
- 8027 - 10357 (-) CAZyme: GH20 Yes
- 10373 - 12112 (-) other Yes
- 12158 - 13939 (-) other Yes
- 13985 - 15760 (-) other Yes
- 15816 - 19175 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 19206 - 20870 (-) CAZyme: GH18 Yes
- 21133 - 21876 (-) CDS No
- 21885 - 23315 (-) CDS No
- 23447 - 27412 (+) TF: DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0035607 No
- 27450 - 28979 (+) CDS No

PUL ID

PUL0467

PubMed

18996345, Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.

Title

Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont.

Author

Martens EC, Chiang HC, Gordon JI

Abstract

The distal human gut is a microbial bioreactor that digests complex carbohydrates. The strategies evolved by gut microbes to sense and process diverse glycans have important implications for the assembly and operation of this ecosystem. The human gut-derived bacterium Bacteroides thetaiotaomicron forages on both host and dietary glycans. Its ability to target these substrates resides in 88 polysaccharide utilization loci (PULs), encompassing 18% of its genome. Whole genome transcriptional profiling and genetic tests were used to define the mechanisms underlying host glycan foraging in vivo and in vitro. PULs that target all major classes of host glycans were identified. However, mucin O-glycans are the principal host substrate foraged in vivo. Simultaneous deletion of five genes encoding ECF-sigma transcription factors, which activate mucin O-glycan utilization, produces defects in bacterial persistence in the gut and in mother-to-offspring transmission. Thus, PUL-mediated glycan catabolism is an important component in gut colonization and may impact microbiota ecology.