PUL ID

PUL0486

PubMed

10613841, Genome Res. 1999 Dec;9(12):1189-97. doi: 10.1101/gr.9.12.1189.

Characterization method

RT-PCR, sugar utilization assay

Genomic accession number

AE007317.1

Nucelotide position range

1679660-1689931

Substrate

raffinose

Loci

spr1708-spr1715

Species

Streptococcus pneumoniae/1313

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- spr1708 AAL00511.1 0 - 1194 (-) AE007317.1:1679660-1680854 -
gtfA spr1709 AAL00512.1 1331 - 2774 (-) AE007317.1:1680991-1682434 2.4.1.5
msmG spr1710 AAL00513.1 3131 - 3965 (-) AE007317.1:1682791-1683625 -
msmF spr1711 AAL00514.1 3978 - 4869 (-) AE007317.1:1683638-1684529 -
msmE spr1712 AAL00515.1 4858 - 6118 (-) AE007317.1:1684518-1685778 -
aga spr1713 AAL00516.1 6209 - 8372 (-) AE007317.1:1685869-1688032 3.2.1.22
msmR spr1714 AAL00517.1 8479 - 9340 (+) AE007317.1:1688139-1689000 -
birA spr1715 AAL00518.1 9336 - 10272 (+) AE007317.1:1688996-1689932 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1194 (-) TC: gnl|TC-DB|Q97NW6|9.B.67.1.2 Yes
gtfA 1332 - 2774 (-) CAZyme: GH13|GH13_18 Yes
msmG 3132 - 3965 (-) TC: gnl|TC-DB|Q00751|3.A.1.1.28 Yes
msmF 3979 - 4869 (-) TC: gnl|TC-DB|Q00750|3.A.1.1.28 Yes
msmE 4859 - 6118 (-) TC: gnl|TC-DB|Q00749|3.A.1.1.28 Yes
aga 6210 - 8372 (-) CAZyme: GH36 Yes
msmR 8480 - 9340 (+) TF: DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0036286,DBD-SUPERFAMILY|0035607 No
birA 9337 - 10272 (+) TF: DBD-Pfam|HTH_11,DBD-SUPERFAMILY|0039384 No

PUL ID

PUL0486

PubMed

10613841, Genome Res. 1999 Dec;9(12):1189-97. doi: 10.1101/gr.9.12.1189.

Title

Regulation of the alpha-galactosidase activity in Streptococcus pneumoniae: characterization of the raffinose utilization system.

Author

Rosenow C, Maniar M, Trias J

Abstract

A 10.2-kb gene region was identified in the Streptococcus pneumoniae genome sequence that contains eight genes involved in regulation and metabolism of raffinose. The genes rafR and rafS are transcribed as one operon, and their gene products regulate the raffinose-dependent stimulation of a divergently transcribed second promoter (P(A)) directing the expression of aga, the structural gene for alpha-galactosidase. Raffinose-mediated transcription from P(A) results in a 500-fold increase in alpha-galactosidase activity in the cell. A third promoter within the cluster is responsible for the transcription of the remaining five genes (rafE, rafF, rafG, gtfA, and rafX), whose gene products might be involved in transport and metabolism of raffinose. The presence of additional internal promoters cannot be excluded. The aga promoter P(A) is negatively regulated by the presence of sucrose in the growth medium. Consistent with catabolite repression (CR), a DNA sequence with high homology to the CRE (cis-active element) was identified upstream of the aga promoter. Sucrose-mediated CR depends on the phosphoenolpyruvate: sucrose phosphotransferase system (PTS) but is unaffected by a mutation in a gene encoding a homolog of the CRE regulatory protein CcpA.