dbCAN-PUL

PUL ID	PUL0569
PubMed	11739774, Microbiology (Reading). 2001 Dec;147(Pt 12):3413-9. doi: 10.1099/00221287-147-12-3413.
Characterization method	clone and expression,enzyme activity assay,Northern Blot
Genomic accession number	NC_000964.3
Nucelotide position range	3536012-3540727
Substrate	levan
Loci	sacB-yveB-yveA
Species	Bacillus subtilis/1423
Degradation or Biosynthesis	degradation

PUL ID	PUL0569
PubMed	11739774, Microbiology (Reading). 2001 Dec;147(Pt 12):3413-9. doi: 10.1099/00221287-147-12-3413.
Title	yveB, Encoding endolevanase LevB, is part of the sacB-yveB-yveA levansucrase tricistronic operon in Bacillus subtilis.
Author	Pereira Y, Petit-Glatron MF, Chambert R
Abstract	Transcription of sacB, yveB and yveA, three clustered genes on the Bacillus subtilis chromosome, is simultaneously induced by sucrose. Northern blotting analyses with specific probes showed three distinct mRNAs: a monocistronic 1.7 kb sacB mRNA, a bicistronic 3.3 kb sacB-yveB mRNA and a tricistronic 4.9 kb sacB-yveB-yveA mRNA. These results indicate that sacB, encoding levansucrase, is the proximal gene of a sucrose-inducible operon that includes the two other genes. The yield of the full-length transcript is lower than that of the bicistronic transcript, whose yield is itself lower than that of the monocistronic transcript. This suggested that the 3' terminal parts of sacB and yveB genes worked as internal terminator structures. The protein encoded by yveB, which remains anchored to the membrane, displays an endolevanase activity, which, coupled with exolevanase activity of SacB, leads to a complete degradation of levan, a branched fructosyl polymer. It is proposed to rename yveB as levB.