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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0572

PubMed

8335634, J Bacteriol. 1993 Aug;175(15):4780-9. doi: 10.1128/jb.175.15.4780-4789.1993.

Characterization method

enzyme activity assay

Genomic accession number

CP007224.1

Nucelotide position range

1516668-1533320

Substrate

alginate

Loci

algAFJILXGEK448D

Species

Pseudomonas aeruginosa/287

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

algA PA96_1392 AHW69886.1 0 - 1446 (-) CP007224.1:1516668-1518114 -
algF PA96_1393 AHW69887.1 1643 - 2294 (-) CP007224.1:1518311-1518962 -
algJ PA96_1394 AHW69888.1 2366 - 3542 (-) CP007224.1:1519034-1520210 -
algI PA96_1395 AHW69889.1 3556 - 5119 (-) CP007224.1:1520224-1521787 -
algL PA96_1396 AHW69890.1 5360 - 6464 (-) CP007224.1:1522028-1523132 -
algX PA96_1397 AHW69891.1 6467 - 7892 (-) CP007224.1:1523135-1524560 -
algG PA96_1398 AHW69892.1 7904 - 9536 (-) CP007224.1:1524572-1526204 -
algE PA96_1399 AHW69893.1 9556 - 11029 (-) CP007224.1:1526224-1527697 -
algK PA96_1400 AHW69894.1 11025 - 12453 (-) CP007224.1:1527693-1529121 -
alg44 PA96_1401 AHW69895.1 12466 - 13636 (-) CP007224.1:1529134-1530304 -
alg8 PA96_1402 AHW69896.1 13718 - 15200 (-) CP007224.1:1530386-1531868 -
algD PA96_1403 AHW69897.1 15342 - 16653 (-) CP007224.1:1532010-1533321 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

algA 1 - 1446 (-) STP: STP|AraC_binding No
algF 1644 - 2294 (-) CDS No
algJ 2367 - 3542 (-) CDS No
algI 3557 - 5119 (-) TC: gnl|TC-DB|P39580|2.A.50.2.1 Yes
algL 5361 - 6464 (-) CAZyme: PL5|PL5_1 Yes
algX 6468 - 7892 (-) other Yes
algG 7905 - 9536 (-) other Yes
algE 9557 - 11029 (-) TC: gnl|TC-DB|P18895|1.B.13.1.1 Yes
algK 11026 - 12453 (-) other Yes
alg44 12467 - 13636 (-) other Yes
alg8 13719 - 15200 (-) TC: gnl|TC-DB|Q52463|4.D.1.1.9 Yes
algD 15343 - 16653 (-) CDS No

PUL ID

PUL0572

PubMed

8335634, J Bacteriol. 1993 Aug;175(15):4780-9. doi: 10.1128/jb.175.15.4780-4789.1993.

Title

Characterization of the Pseudomonas aeruginosa alginate lyase gene (algL): cloning, sequencing, and expression in Escherichia coli.

Author

Schiller NL, Monday SR, Boyd CM, Keen NT, Ohman DE

Abstract

Mucoid strains of Pseudomonas aeruginosa produce a viscous exopolysaccharide called alginate and also express alginate lyase activity which can degrade this polymer. By transposon mutagenesis and gene replacement techniques, the algL gene encoding a P. aeruginosa alginate lyase enzyme was found to reside between algG and algA within the alginate biosynthetic gene cluster at 35 min on the P. aeruginosa chromosome. DNA sequencing data for algL predicted a protein product of ca. 41 kDa, including a 27-amino-acid signal sequence, which would be consistent with its possible localization in the periplasmic space. Expression of the algL gene in Escherichia coli cells resulted in the expression of alginate lyase activity and the appearance of a new protein of ca. 39 kDa detected on sodium dodecyl sulfate-polyacrylamide gels. In mucoid P. aeruginosa strains, expression of algL was regulated by AlgB, which also controls expression of other genes within the alginate gene cluster. Since alginate lyase activity is associated with the ability to produce and secrete alginate polymers, alginate lyase may play a role in alginate production.