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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0585

PubMed

21778207, Microbiology (Reading). 2011 Oct;157(Pt 10):2854-2861. doi: 10.1099/mic.0.051359-0. Epub 2011 Jul 21.

Characterization method

microarray,gene deletion mutant and growth assay,beta-galactosidase assays

Genomic accession number

CP000410.2

Nucelotide position range

274549-281092

Substrate

cellobiose

Loci

celA-celD

Species

Streptococcus pneumoniae/1313

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

bglA-1 SPD_0277 ABJ53661.1 0 - 1437 (+) CP000410.2:274549-275986 3.2.1.21
- SPD_0278 ABJ53972.1 1488 - 1728 (+) CP000410.2:276037-276277 -
- SPD_0279 ABJ55373.1 1857 - 2172 (+) CP000410.2:276406-276721 -
- SPD_0280 ABJ54823.1 2295 - 4269 (+) CP000410.2:276844-278818 -
- SPD_0281 ABJ54026.1 4278 - 4593 (+) CP000410.2:278827-279142 2.7.1.-
- SPD_0282 ABJ53797.1 4611 - 5118 (+) CP000410.2:279160-279667 -
- SPD_0283 ABJ54842.1 5197 - 6544 (+) CP000410.2:279746-281093 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

bglA-1 1 - 1437 (+) CAZyme: GH1 Yes
- 1489 - 1728 (+) TC: gnl|TC-DB|C3WG44|2.A.123.2.3 Yes
- 1858 - 2172 (+) other Yes
- 2296 - 4269 (+) STP: STP|HTH_11,STP|Mga Yes
- 4279 - 4593 (+) TC: gnl|TC-DB|P46319|4.A.3.2.2 Yes
- 4612 - 5118 (+) other Yes
- 5198 - 6544 (+) TC: gnl|TC-DB|Q72XQ0|4.A.3.2.8 Yes

PUL ID

PUL0585

PubMed

21778207, Microbiology (Reading). 2011 Oct;157(Pt 10):2854-2861. doi: 10.1099/mic.0.051359-0. Epub 2011 Jul 21.

Title

CelR-mediated activation of the cellobiose-utilization gene cluster in Streptococcus pneumoniae.

Author

Shafeeq S, Kloosterman TG, Kuipers OP

Abstract

The human pathogen Streptococcus pneumoniae harbours many genes encoding phosphotransferase systems and sugar ABC (ATP-binding cassette) transporters, including systems for the utilization of the beta-glucoside sugar cellobiose. In this study, we show that the transcriptional regulator CelR, which has previously been found to be important for pneumococcal virulence, activates the expression of the cellobiose-utilization gene cluster (cel locus) of S. pneumoniae. Expression directed by the two promoters present in the cel locus was increased in the presence of cellobiose as sole carbon source in the medium, while expression decreased in the presence of glucose in the medium. Furthermore, we have predicted a 22 bp putative CelR regulatory site (5'-YTTTCCWTAWCAWTWAGGAAAA-3') in the promoters of celA and celB, and in silico analysis showed that it is highly conserved in other pathogenic streptococci as well. Promoter truncations of celA and celB, where the half or full CelR regulatory site was deleted, confirmed that the CelR-binding site in PcelA and PcelB is functional. Transcriptome studies with the celR mutant and in silico prediction of the CelR regulatory site in the entire D39 genome sequence show that the cel locus is the only cluster of genes under the direct control of CelR. Therefore, CelR is a regulator dedicated to the cellobiose-dependent transcriptional activation of the cel locus.