dbCAN-PUL

PUL ID	PUL0586
PubMed	23047109, Biosci Biotechnol Biochem. 2012;76(10):1920-4. doi: 10.1271/bbb.120403. Epub 2012 Oct 7.
Characterization method	enzyme activity assay
Genomic accession number	AB728624.1
Nucelotide position range	585-5032
Substrate	chitin
Loci	ybfMN-ctb
Species	Serratia marcescens/615
Degradation or Biosynthesis	degradation

PUL ID	PUL0586
PubMed	23047109, Biosci Biotechnol Biochem. 2012;76(10):1920-4. doi: 10.1271/bbb.120403. Epub 2012 Oct 7.
Title	Regulation of chitinase production by the 5'-untranslated region of the ybfM in Serratia marcescens 2170.
Author	Toratani T, Suzuki K, Shimizu M, Sugimoto H, Watanabe T
Abstract	Serratia marcescens 2170 produces three chitinases and the chitin-binding protein CBP21, and efficiently degrades insoluble and crystalline chitin. The three chitinases and CBP21 are induced by N,N'-diacetylchitobiose [(GlcNAc)(2)], the major product of chitin hydrolysis by S. marcescens chitinases. We have found that uptake of both (GlcNAc)(2) and N-acetylglucosamine (GlcNAc) is important for the efficient utilization of (GlcNAc)(2) because (GlcNAc)(2) is less efficiently fermented by S. marcescens 2170 in the absence of chitobiase. In order to determine the mechanism of utilization of the degradation products of chitin by S. marcescens, chitobiase deficient transposon mutants were screened. A transposon present in chitobiase-deficient mutants was inserted into the ybfMN-ctb cluster. The mutants produced chitinases, except for TT327, in which a transposon was inserted into the 5'-untranslated region (5'-UTR) of ybfM. Ectopic expression of this region in TT327 restored chitinase production. These results indicate that the 5'-UTR of ybfM is important for chitinase induction in S. marcescens.