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PlantCAZyme
Cite: NAR/gkaa742 2020

Because CGCFinder predicted no CGC for this PUL, the gene cluster depicted below contains dbCAN2 and CGC signature predictions for all genes in the PUL, instead of a predicted CGC.


PUL General Information

Literature Information

PUL ID

PUL0591

PubMed

23667565, PLoS One. 2013 May 8;8(5):e63025. doi: 10.1371/journal.pone.0063025. Print 2013.

Characterization method

growth assay,Northern Blot

Genomic accession number

NC_000964.3

Nucelotide position range

3594242-3598020

Substrate

N-acetylglucosamine

Loci

hprk-nagA-nagBA-nagR

Species

Bacillus subtilis/1423

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

hprK BSU_35000 NP_391380.1 0 - 933 (-) NC_000964.3:3594242-3595175 2.7.4.-, 2.7.11.-
nagA BSU_35010 NP_391381.1 1114 - 2305 (+) NC_000964.3:3595356-3596547 3.5.1.25
nagBA BSU_35020 NP_391382.1 2301 - 3030 (+) NC_000964.3:3596543-3597272 3.5.99.6
nagR BSU_35030 NP_391383.1 3047 - 3779 (+) NC_000964.3:3597289-3598021 -

Cluster number

0

Gene name

Gene position

Gene type

Found by CGCFinder?

hprK 1 - 933 (-) CDS No
nagA 1115 - 2305 (+) CAZyme: CE9 No
nagBA 2302 - 3030 (+) CDS No
nagR 3048 - 3779 (+) TF: DBD-Pfam|GntR,DBD-SUPERFAMILY|0037767 No

PUL ID

PUL0591

PubMed

23667565, PLoS One. 2013 May 8;8(5):e63025. doi: 10.1371/journal.pone.0063025. Print 2013.

Title

The use of amino sugars by Bacillus subtilis: presence of a unique operon for the catabolism of glucosamine.

Author

Gaugue I, Oberto J, Putzer H, Plumbridge J

Abstract

B. subtilis grows more rapidly using the amino sugar glucosamine as carbon source, than with N-acetylglucosamine. Genes for the transport and metabolism of N-acetylglucosamine (nagP and nagAB) are found in all the sequenced Bacilli (except Anoxybacillus flavithermus). In B. subtilis there is an additional operon (gamAP) encoding second copies of genes for the transport and catabolism of glucosamine. We have developed a method to make multiple deletion mutations in B. subtilis employing an excisable spectinomycin resistance cassette. Using this method we have analysed the contribution of the different genes of the nag and gam operons for their role in utilization of glucosamine and N-acetylglucosamine. Faster growth on glucosamine is due to the presence of the gamAP operon, which is strongly induced by glucosamine. Although the gamA and nagB genes encode isozymes of GlcN6P deaminase, catabolism of N-acetylglucosamine relies mostly upon the gamA gene product. The genes for use of N-acetylglucosamine, nagAB and nagP, are repressed by YvoA (NagR), a GntR family regulator, whose gene is part of the nagAB yvoA(nagR) operon. The gamAP operon is repressed by YbgA, another GntR family repressor, whose gene is expressed divergently from gamAP. The nagAB yvoA synton is found throughout the Bacilli and most firmicutes. On the other hand the ybgA-gamAP synton, which includes the ybgB gene for a small protein of unknown provenance, is only found in B. subtilis (and a few very close relatives). The origin of ybgBA-gamAP grouping is unknown but synteny analysis suggests lateral transfer from an unidentified donor. The presence of gamAP has enabled B. subtilis to efficiently use glucosamine as carbon source.