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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0664

PubMed

34340552, mBio. 2021 Aug 31;12(4):e0136821. doi: 10.1128/mBio.01368-21. Epub 2021 Aug 3.

Characterization method

thin layer chromatography,clone and expression,recombinant protein expression

Genomic accession number

NZ_DS990119.1

Nucelotide position range

268931-297885

Substrate

arabinogalactan protein

Loci

BACPLE_RS02415-BACPLE_RS02485

Species

Bacteroides plebeius DSM17135/310297

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BACPLE_RS02415 WP_050792054.1 0 - 4227 (-) NZ_DS990119.1:268931-273158 2.7.13.3
- BACPLE_RS02420 WP_081458090.1 4546 - 6031 (-) NZ_DS990119.1:273477-274962 -
- BACPLE_RS02425 WP_148374629.1 6214 - 8047 (+) NZ_DS990119.1:275145-276978 -
- BACPLE_RS02430 WP_007558596.1 8064 - 9495 (+) NZ_DS990119.1:276995-278426 -
- BACPLE_RS02435 WP_007558600.1 9588 - 11025 (+) NZ_DS990119.1:278519-279956 -
- BACPLE_RS02440 WP_007558602.1 11042 - 13022 (+) NZ_DS990119.1:279973-281953 -
- BACPLE_RS02445 WP_007558604.1 13132 - 14512 (+) NZ_DS990119.1:282063-283443 -
- BACPLE_RS02450 WP_007558606.1 14611 - 16669 (-) NZ_DS990119.1:283542-285600 -
- BACPLE_RS02455 WP_007558608.1 16686 - 18060 (-) NZ_DS990119.1:285617-286991 -
- BACPLE_RS02460 WP_007558610.1 18084 - 19089 (-) NZ_DS990119.1:287015-288020 -
- BACPLE_RS02465 WP_007558613.1 19137 - 19932 (-) NZ_DS990119.1:288068-288863 -
- BACPLE_RS19600 WP_007558614.1 19937 - 20237 (-) NZ_DS990119.1:288868-289168 -
- BACPLE_RS02470 WP_148374630.1 20273 - 21080 (-) NZ_DS990119.1:289204-290011 -
- BACPLE_RS02475 WP_007558618.1 21222 - 24147 (-) NZ_DS990119.1:290153-293078 -
- BACPLE_RS02480 WP_167535712.1 24263 - 25916 (-) NZ_DS990119.1:293194-294847 -
- BACPLE_RS02485 WP_007558622.1 25934 - 28955 (-) NZ_DS990119.1:294865-297886 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 4227 (-) TF: DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0035607 No
- 4547 - 6031 (-) CAZyme: GH49|GH49|3.2.1.- Yes
- 6215 - 8047 (+) CAZyme: GH2|GH2 Yes
- 8065 - 9495 (+) other Yes
- 9589 - 11025 (+) CAZyme: GH27|GH27 Yes
- 11043 - 13022 (+) CAZyme: GH43| GH43 Yes
- 13133 - 14512 (+) other Yes
- 14612 - 16669 (-) CAZyme: GH36 Yes
- 16687 - 18060 (-) CAZyme: GH49|GH49 Yes
- 18085 - 19089 (-) CAZyme: GH43 Yes
- 19138 - 19932 (-) other Yes
- 19938 - 20237 (-) other Yes
- 20274 - 21080 (-) other Yes
- 21223 - 24147 (-) CAZyme: GH2|GH2 Yes
- 24264 - 25916 (-) other Yes
- 25935 - 28955 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes

PUL ID

PUL0664

PubMed

34340552, mBio. 2021 Aug 31;12(4):e0136821. doi: 10.1128/mBio.01368-21. Epub 2021 Aug 3.

Title

Sulfation of Arabinogalactan Proteins Confers Privileged Nutrient Status to Bacteroides plebeius.

Author

Munoz-Munoz J, Ndeh D, Fernandez-Julia P, Walton G, Henrissat B, Gilbert HJ

Abstract

The human gut microbiota (HGM) contributes to the physiology and health of its host. The health benefits provided by dietary manipulation of the HGM require knowledge of how glycans, the major nutrients available to this ecosystem, are metabolized. Arabinogalactan proteins (AGPs) are a ubiquitous feature of plant polysaccharides available to the HGM. Although the galactan backbone and galactooligosaccharide side chains of AGPs are conserved, the decorations of these structures are highly variable. Here, we tested the hypothesis that these variations in arabinogalactan decoration provide a selection mechanism for specific Bacteroides species within the HGM. The data showed that only a single bacterium, B. plebeius, grew on red wine AGP (Wi-AGP) and seaweed AGP (SW-AGP) in mono- or mixed culture. Wi-AGP thus acts as a privileged nutrient for a Bacteroides species within the HGM that utilizes marine and terrestrial plant glycans. The B. plebeius polysaccharide utilization loci (PULs) upregulated by AGPs encoded a polysaccharide lyase, located in the enzyme family GH145, which hydrolyzed Rha-Glc linkages in Wi-AGP. Further analysis of GH145 identified an enzyme with two active sites that displayed glycoside hydrolase and lyase activities, respectively, which conferred substrate flexibility for different AGPs. The AGP-degrading apparatus of B. plebeius also contained a sulfatase, BpS1_8, active on SW-AGP and Wi-AGP, which played a pivotal role in the utilization of these glycans by the bacterium. BpS1_8 enabled other Bacteroides species to access the sulfated AGPs, providing a route to introducing privileged nutrient utilization into probiotic and commensal organisms that could improve human health. IMPORTANCE Dietary manipulation of the HGM requires knowledge of how glycans available to this ecosystem are metabolized. The variable structures that decorate the core component of plant AGPs may influence their utilization by specific organisms within the HGM. Here, we evaluated the ability of Bacteroides species to utilize a marine and terrestrial AGP. The data showed that a single bacterium, B. plebeius, grew on Wi-AGP and SW-AGP in mono- or mixed culture. Wi-AGP is thus a privileged nutrient for a Bacteroides species that utilizes marine and terrestrial plant glycans. A key component of the AGP-degrading apparatus of B. plebeius is a sulfatase that conferred the ability of the bacterium to utilize these glycans. The enzyme enabled other Bacteroides species to access the sulfated AGPs, providing a route to introducing privileged nutrient utilization into probiotic and commensal organisms that could improve human health.