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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0669

PubMed

34480044, Sci Rep. 2021 Sep 3;11(1):17662. doi: 10.1038/s41598-021-96659-z.

Characterization method

clone,high performance anion exchange chromatography,enzymatic product analysis

Genomic accession number

NZ_GL622542.1

Nucelotide position range

196589-248685

Substrate

xylan

Loci

HMPREF1016_RS10680-HMPREF1016_RS10800

Species

Bacteroides eggerthii 1_2_48FAA/28111

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- HMPREF1016_RS10680 WP_004294234.1 0 - 1710 (+) NZ_GL622542.1:196589-198299 -
- HMPREF1016_RS10685 WP_004294235.1 1753 - 3385 (+) NZ_GL622542.1:198342-199974 -
- HMPREF1016_RS10690 WP_004289614.1 3430 - 4927 (+) NZ_GL622542.1:200019-201516 -
- HMPREF1016_RS10695 WP_004294236.1 5016 - 5988 (+) NZ_GL622542.1:201605-202577 -
- HMPREF1016_RS10700 WP_017141108.1 6121 - 8077 (+) NZ_GL622542.1:202710-204666 -
- HMPREF1016_RS10705 WP_004294238.1 8357 - 12455 (+) NZ_GL622542.1:204946-209044 -
- HMPREF1016_RS10710 WP_004294239.1 12530 - 15113 (-) NZ_GL622542.1:209119-211702 -
- HMPREF1016_RS10715 WP_232286955.1 15204 - 17355 (-) NZ_GL622542.1:211793-213944 -
- HMPREF1016_RS20970 WP_232286957.1 17344 - 18457 (-) NZ_GL622542.1:213933-215046 -
- HMPREF1016_RS19820 WP_004294240.1 18481 - 20923 (-) NZ_GL622542.1:215070-217512 -
- HMPREF1016_RS10725 WP_004294241.1 20986 - 22933 (-) NZ_GL622542.1:217575-219522 -
- HMPREF1016_RS10730 WP_004294242.1 23176 - 26284 (+) NZ_GL622542.1:219765-222873 -
- HMPREF1016_RS10735 WP_004289604.1 26545 - 28999 (+) NZ_GL622542.1:223134-225588 -
- HMPREF1016_RS10740 WP_050793236.1 29062 - 31504 (-) NZ_GL622542.1:225651-228093 -
- HMPREF1016_RS10745 WP_029429358.1 31547 - 32696 (-) NZ_GL622542.1:228136-229285 -
- HMPREF1016_RS10750 WP_004289601.1 33007 - 33646 (-) NZ_GL622542.1:229596-230235 -
- HMPREF1016_RS10755 WP_017141103.1 33660 - 34029 (-) NZ_GL622542.1:230249-230618 -
- HMPREF1016_RS10760 WP_004289599.1 34339 - 34867 (+) NZ_GL622542.1:230928-231456 -
- HMPREF1016_RS10765 WP_004289598.1 34907 - 36896 (-) NZ_GL622542.1:231496-233485 -
- HMPREF1016_RS10770 WP_004294246.1 37000 - 39160 (-) NZ_GL622542.1:233589-235749 -
- HMPREF1016_RS10775 WP_004289596.1 39171 - 40218 (-) NZ_GL622542.1:235760-236807 -
- HMPREF1016_RS10780 WP_004289595.1 40247 - 42056 (-) NZ_GL622542.1:236836-238645 -
- HMPREF1016_RS10785 WP_029429356.1 42072 - 44937 (-) NZ_GL622542.1:238661-241526 -
- HMPREF1016_RS10790 WP_004294248.1 44981 - 46925 (-) NZ_GL622542.1:241570-243514 -
- HMPREF1016_RS10795 WP_004289592.1 46936 - 50092 (-) NZ_GL622542.1:243525-246681 -
- HMPREF1016_RS10800 WP_004289591.1 50363 - 52097 (-) NZ_GL622542.1:246952-248686 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1710 (+) CAZyme: GH35| GH35 Yes
- 1754 - 3385 (+) CAZyme: CE20| CE20 Yes
- 3431 - 4927 (+) TC: gnl|TC-DB|P31435|2.A.2.3.4 Yes
- 5017 - 5988 (+) CAZyme: GH43_1|GH43_1 Yes
- 6122 - 8077 (+) CAZyme: GH67| GH67 Yes
- 8358 - 12455 (+) TF: DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0036286,DBD-SUPERFAMILY|0035607 Yes
- 12531 - 15113 (-) CAZyme: GH115|GH115 Yes
- 15205 - 17355 (-) CAZyme: GH95| GH95|CE6 Yes
- 17345 - 18457 (-) CAZyme: CE6|GH95|CE6 Yes
- 18482 - 20923 (-) CAZyme: CE15|GH8| GH8|CE15 Yes
- 20987 - 22933 (-) CAZyme: GH97| GH97 Yes
- 23177 - 26284 (+) TF: DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0036286,DBD-SUPERFAMILY|0035607 Yes
- 26546 - 28999 (+) CAZyme: GH31|GH31 Yes
- 29063 - 31504 (-) CAZyme: CBM91| GH43_10|GH43_10 Yes
- 31548 - 32696 (-) CAZyme: CBM48|CE0|CE1 Yes
- 33008 - 33646 (-) other Yes
- 33661 - 34029 (-) other Yes
- 34340 - 34867 (+) other Yes
- 34908 - 36896 (-) CAZyme: GH5_21| GH5_21 Yes
- 37001 - 39160 (-) CAZyme: GH10|GH10 Yes
- 39172 - 40218 (-) other Yes
- 40248 - 42056 (-) other Yes
- 42073 - 44937 (-) other Yes
- 44982 - 46925 (-) other Yes
- 46937 - 50092 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 50364 - 52097 (-) CAZyme: GH43_12|CBM91|GH43_12 Yes

PUL ID

PUL0669

PubMed

34480044, Sci Rep. 2021 Sep 3;11(1):17662. doi: 10.1038/s41598-021-96659-z.

Title

Characterization of a novel multidomain CE15-GH8 enzyme encoded by a polysaccharide utilization locus in the human gut bacterium Bacteroides eggerthii.

Author

Kmezik C, Krska D, Mazurkewich S, Larsbrink J

Abstract

Bacteroidetes are efficient degraders of complex carbohydrates, much thanks to their use of polysaccharide utilization loci (PULs). An integral part of PULs are highly specialized carbohydrate-active enzymes, sometimes composed of multiple linked domains with discrete functions-multicatalytic enzymes. We present the biochemical characterization of a multicatalytic enzyme from a large PUL encoded by the gut bacterium Bacteroides eggerthii. The enzyme, BeCE15A-Rex8A, has a rare and novel architecture, with an N-terminal carbohydrate esterase family 15 (CE15) domain and a C-terminal glycoside hydrolase family 8 (GH8) domain. The CE15 domain was identified as a glucuronoyl esterase (GE), though with relatively poor activity on GE model substrates, attributed to key amino acid substitutions in the active site compared to previously studied GEs. The GH8 domain was shown to be a reducing-end xylose-releasing exo-oligoxylanase (Rex), based on having activity on xylooligosaccharides but not on longer xylan chains. The full-length BeCE15A-Rex8A enzyme and the Rex domain were capable of boosting the activity of a commercially available GH11 xylanase on corn cob biomass. Our research adds to the understanding of multicatalytic enzyme architectures and showcases the potential of discovering novel and atypical carbohydrate-active enzymes from mining PULs.