Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0003 RT-PCR xylan Bacillus subtilis 26559526
Metabolic potential of Bacillus subtilis 168 for the direct conversion of xylans to fermentation products. Appl Microbiol Biotechnol. 2016 Feb;100(3):1501-1510. doi: 10.1007/s00253-015-7124-x. Epub 2015 Nov 12.
2016 Feb degradation 2 2 GH30, GH30_8, GH43_16, CBM6
PUL0005 enzyme activity assay, crystallization beta-glucan, sophorose, laminaribiose Listeria innocua 26886583
Functional and Structural Analysis of a beta-Glucosidase Involved in beta-1,2-Glucan Metabolism in Listeria innocua. PLoS One. 2016 Feb 17;11(2):e0148870. doi: 10.1371/journal.pone.0148870. eCollection 2016.
2016 degradation 2 1 GH94, GH3
PUL0010 enzyme activity assay, liquid chromatography and mass spectrometry xylan Geobacillus thermodenitrificans 28616644
Synergistic hydrolysis of xylan using novel xylanases, beta-xylosidases, and an alpha-L-arabinofuranosidase from Geobacillus thermodenitrificans NG80-2. Appl Microbiol Biotechnol. 2017 Aug;101(15):6023-6037. doi: 10.1007/s00253-017-8341-2. Epub 2017 Jun 14.
2017 Aug degradation 42 9 GH43, GH43_11, GH10, GH39, GH67, GH10, CBM22, GH52, CE4, GH51
PUL0049 fosmid library screen beta-glucan, galactomannan feces metagenome 29601586
Two new gene clusters involved in the degradation of plant cell wall from the fecal microbiota of Tunisian dromedary. PLoS One. 2018 Mar 30;13(3):e0194621. doi: 10.1371/journal.pone.0194621. eCollection 2018.
2018 degradation 29 6 GH43_17, GH26, GH16
PUL0078 enzyme activity assay xylan, xylose Caldicellulosiruptor sp. Rt8B.4 8920183
Cloning, sequencing and overexpression in Escherichia coli of a xylanase gene, xynA from the thermophilic bacterium Rt8B.4 genus Caldicellulosiruptor. Appl Microbiol Biotechnol. 1996 Mar;45(1-2):86-93. doi: 10.1007/s002530050653.
1996 Mar degradation 6 1 CBM22, GH10
PUL0118 qRT-PCR, affinity gel electrophoresis, isothermal titration calorimetry laminarin, beta-glucan Bacteroides uniformis 32265336
Synergy between Cell Surface Glycosidases and Glycan-Binding Proteins Dictates the Utilization of Specific Beta(1,3)-Glucans by Human Gut Bacteroides. mBio. 2020 Apr 7;11(2):e00095-20. doi: 10.1128/mBio.00095-20.
2020 Apr 7 degradation 7 3 CBM6, GH3, GH158, GH16
PUL0125 affinity gel electrophoresis, substrate binding assay, RNA-Seq beta-glucan Bacteroides ovatus 31062073, 32801182
Surface glycan-binding proteins are essential for cereal beta-glucan utilization by the human gut symbiont Bacteroides ovatus. Sharing a beta-Glucan Meal: Transcriptomic Eavesdropping on a Bacteroides ovatus-Subdoligranulum variabile-Hungatella hathewayi Consortium. Cell Mol Life Sci. 2019 Nov;76(21):4319-4340. doi: 10.1007/s00018-019-03115-3. Epub 2019 May 6. Appl Environ Microbiol. 2020 Oct 1;86(20):e01651-20. doi: 10.1128/AEM.01651-20. Print 2020 Oct 1.
2019 Nov,2020 Oct 1 degradation 6 2 GH16, GH3
PUL0170 enzyme activity assay beta-glucan Bacteroides ovatus 29141231
Molecular Mechanism by which Prominent Human Gut Bacteroidetes Utilize Mixed-Linkage Beta-Glucans, Major Health-Promoting Cereal Polysaccharides. Cell Rep. 2017 Nov 14;21(7):2030. doi: 10.1016/j.celrep.2017.11.013.
2017 Nov 14 degradation 7 3 GH3, GH16, GH3
PUL0187 qRT-PCR, enzyme activity assay beta-glucan Paenibacillus sp. JDR-2 26746717
A 1,3-1,4-beta-Glucan Utilization Regulon in Paenibacillus sp. Strain JDR-2. Appl Environ Microbiol. 2016 Jan 8;82(6):1789-1798. doi: 10.1128/AEM.03526-15.
2016 Jan 8 degradation 7 2 CBM4, CBM54, CBM6, GH16, GH16
PUL0227 enzyme activity assay, substrate binding assay xylan Caldanaerobius polysaccharolyticus 22918832
Biochemical and structural insights into xylan utilization by the thermophilic bacterium Caldanaerobius polysaccharolyticus. J Biol Chem. 2012 Oct 12;287(42):34946-34960. doi: 10.1074/jbc.M112.391532. Epub 2012 Aug 22.
2012 Oct 12 degradation 12 1 GH67, GH3
PUL0262 RNA-Seq xylan, arabinoxylan Bacteroides cellulosilyticus 23976882
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.
2013 degradation 12 6 GH10, CBM4, GH10, GH5_21, GH5, CE1, CE0, CE6, GH95, GH8
PUL0263 RNA-Seq xylan, arabinoxylan Bacteroides cellulosilyticus 23976882, 30674645
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. Wood-Derived Dietary Fibers Promote Beneficial Human Gut Microbiota. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20. mSphere. 2019 Jan 23;4(1):e00554-18. doi: 10.1128/mSphere.00554-18.
2013,2019 Jan 23 degradation 5 1 GH10, CBM4, CBM22
PUL0289 enzyme activity assay xylan Flavobacterium johnsoniae 29588659
A novel acetyl xylan esterase enabling complete deacetylation of substituted xylans. Biotechnol Biofuels. 2018 Mar 22;11:74. doi: 10.1186/s13068-018-1074-3. eCollection 2018.
2018 degradation 12 7 GH97, GH3, CBM22, GH43_10, GH43, GH146, GH115, CE0, CE6, CE1, GH43_12
PUL0308 microarray, enzyme activity assay, high performance anion exchange chromatography, mass spectrometry, RNA-seq beta-glucan Bacteroides ovatus 29020628, 22205877, 32801182
Molecular Mechanism by which Prominent Human Gut Bacteroidetes Utilize Mixed-Linkage Beta-Glucans, Major Health-Promoting Cereal Polysaccharides. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Sharing a beta-Glucan Meal: Transcriptomic Eavesdropping on a Bacteroides ovatus-Subdoligranulum variabile-Hungatella hathewayi Consortium. Cell Rep. 2017 Oct 10;21(2):417-430. doi: 10.1016/j.celrep.2017.09.049. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. Appl Environ Microbiol. 2020 Oct 1;86(20):e01651-20. doi: 10.1128/AEM.01651-20. Print 2020 Oct 1.
2017 Oct 10,2011 Dec,2020 Oct 1 degradation 5 3 GH16, GH3
PUL0325 RT-PCR, enzyme activity assay beta-glucan Bacteroides thetaiotaomicron 28461332
A Bacteroidetes locus dedicated to fungal 1,6-beta-glucan degradation: Unique substrate conformation drives specificity of the key endo-1,6-beta-glucanase. J Biol Chem. 2017 Jun 23;292(25):10639-10650. doi: 10.1074/jbc.M117.787606. Epub 2017 May 1.
2017 Jun 23 degradation 6 2 GH30, GH30_3, GH3
PUL0326 gene deletion mutant and growth assay, enzyme activity assay, thin layer chromatography beta-glucan Bacteroides ovatus 28461332
A Bacteroidetes locus dedicated to fungal 1,6-beta-glucan degradation: Unique substrate conformation drives specificity of the key endo-1,6-beta-glucanase. J Biol Chem. 2017 Jun 23;292(25):10639-10650. doi: 10.1074/jbc.M117.787606. Epub 2017 May 1.
2017 Jun 23 degradation 13 2 GH73
PUL0328 microarray, gas chromatography and mass spectrometry xylan Gramella flava 28261179
Characterization of Potential Polysaccharide Utilization Systems in the Marine Bacteroidetes Gramella Flava JLT2011 Using a Multi-Omics Approach. Front Microbiol. 2017 Feb 14;8:220. doi: 10.3389/fmicb.2017.00220. eCollection 2017.
2017 degradation 10 4 GH127, CBM13, GH43, GH43_26, GH2, GH5, GH5_13
PUL0329 microarray, gas chromatography and mass spectrometry xylan Gramella flava 28261179
Characterization of Potential Polysaccharide Utilization Systems in the Marine Bacteroidetes Gramella Flava JLT2011 Using a Multi-Omics Approach. Front Microbiol. 2017 Feb 14;8:220. doi: 10.3389/fmicb.2017.00220. eCollection 2017.
2017 degradation 25 7 GH43_10, GH43, CBM22, GH43, GH43_12, GH43, GH43_1, GH3, GH67, GH10, GH115, CE15
PUL0332 fosmid library screen, enzyme activity assay, thin layer chromatography carboxymethylcellulose, xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 19 9 GH5, GH5_2, GH5_7, GH5, CE7, GH2, GH94, GH97, GH127, GH127
PUL0333 fosmid library screen, enzyme activity assay, thin layer chromatography carboxymethylcellulose, xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 22 3 GH9, GH31, GH9
PUL0334 fosmid library screen, enzyme activity assay, thin layer chromatography carboxymethylcellulose, xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 23 5 GH9, GH31, GH9, GH9, CE4
PUL0335 fosmid library screen, enzyme activity assay, thin layer chromatography xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 31 4 GH13, GH97, GH3, GH158, GH16
PUL0336 fosmid library screen, enzyme activity assay, thin layer chromatography xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 25 3 GH3, GH158, GH16
PUL0337 fosmid library screen, enzyme activity assay, thin layer chromatography xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 29 3 GH16, GH158, GH3
PUL0338 fosmid library screen, enzyme activity assay, thin layer chromatography xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 34 4 GH16, GH158, GH3, GH97
PUL0339 fosmid library screen, enzyme activity assay, thin layer chromatography xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 24 4 GH97, GH3, GH16, GH20
PUL0340 fosmid library screen, enzyme activity assay, thin layer chromatography carboxymethylcellulose, xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 37 1 GH5_2, GH5
PUL0341 fosmid library screen, enzyme activity assay, thin layer chromatography carboxymethylcellulose, xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 43 3 GH32, GH91, GH5, GH5_2
PUL0342 enzyme activity assay, gene deletion mutant and growth assay xylan Prevotella ruminicola 19304844
Biochemical analysis of a beta-D-xylosidase and a bifunctional xylanase-ferulic acid esterase from a xylanolytic gene cluster in Prevotella ruminicola 23. J Bacteriol. 2009 May;191(10):3328-38. doi: 10.1128/JB.01628-08. Epub 2009 Mar 20.
2009 May degradation 5 3 GH10, CE1, CBM22, GH95, GH3
PUL0343 gene deletion mutant and growth assay, enzyme activity assay, Western Blot, isothermal titration calorimetry carboxymethylcellulose, xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 39 1 GH5, GH5_2
PUL0345 qRT-PCR, enzyme activity assay xylan Bacteroides intestinalis 27681607
Bacteroides intestinalis DSM 17393, a member of the human colonic microbiome, upregulates multiple endoxylanases during growth on xylan. Sci Rep. 2016 Sep 29;6:34360. doi: 10.1038/srep34360.
2016 Sep 29 degradation 31 13 CBM4, GH10, GH35, GH43_12, GH43, GH10, GH43_1, GH43, GH67, GH8, CE6, GH95, CE0, CE1, GH5, GH5_21, GH10, CBM0, GH115
PUL0352 microarray arabinan, xylan, levan, pectin, rhamnogalacturonan Bacteroides thetaiotaomicron 16968696
Functional genomic and metabolic studies of the adaptations of a prominent adult human gut symbiont, Bacteroides thetaiotaomicron, to the suckling period. J Biol Chem. 2006 Nov 24;281(47):36269-79. doi: 10.1074/jbc.M606509200. Epub 2006 Sep 12.
2006 Nov 24 degradation 6 1 GH20
PUL0353 microarray, enzyme activity assay, strcutural analysis, clone and expression arabinan, xylan, levan, pectin, rhamnogalacturonan Bacteroides thetaiotaomicron 16968696, 32060313
Functional genomic and metabolic studies of the adaptations of a prominent adult human gut symbiont, Bacteroides thetaiotaomicron, to the suckling period. Structural basis of mammalian high-mannose N-glycan processing by human gut Bacteroides. J Biol Chem. 2006 Nov 24;281(47):36269-79. doi: 10.1074/jbc.M606509200. Epub 2006 Sep 12. Nat Commun. 2020 Feb 14;11(1):899. doi: 10.1038/s41467-020-14754-7.
2006 Nov 24,2020 Feb 14 degradation 11 3 GH0, GH92, GH92
PUL0357 microarray arabinan, xylan, levan, pectin, rhamnogalacturonan Bacteroides thetaiotaomicron 16968696
Functional genomic and metabolic studies of the adaptations of a prominent adult human gut symbiont, Bacteroides thetaiotaomicron, to the suckling period. J Biol Chem. 2006 Nov 24;281(47):36269-79. doi: 10.1074/jbc.M606509200. Epub 2006 Sep 12.
2006 Nov 24 degradation 3 1 CBM32
PUL0364 enzyme activity assay xylan Xanthomonas campestris pv. campestris 17311090
Plant carbohydrate scavenging through tonB-dependent receptors: a feature shared by phytopathogenic and aquatic bacteria. PLoS One. 2007 Feb 21;2(2):e224. doi: 10.1371/journal.pone.0000224.
2007 Feb 21 degradation 8 4 CBM22, GH10, GH2, GH10, GH43_1
PUL0391 microarray cellulose, xylan, xyloglucan, pectin, mannan Caldicellulosiruptor bescii 21227922
Insights into plant biomass conversion from the genome of the anaerobic thermophilic bacterium Caldicellulosiruptor bescii DSM 6725. Nucleic Acids Res. 2011 Apr;39(8):3240-54. doi: 10.1093/nar/gkq1281. Epub 2011 Jan 11.
2011 Apr degradation 15 10 PL11, CBM35, GH12, CBM3, CBM2, CE12, PL3_1, PL3, CBM66, PL9_1, CBM35, CBM66, PL9, CBM22, GH12, CBM0, CBM3, CBM2, CBM1, GH48, GH10, CBM9, GH44, CBM35, GH12, CBM44, CBM3, CBM2, CBM76, CBM10, GH5, GH5_8, CBM35, GH12, CBM5, CBM3, CBM2, GH74, CBM1, GH48, GT39, CBM3, GH9, GH5_8, CBM3, GH5_1, GH5_8, CBM3, GH48, GH9
PUL0392 RT-PCR, qPCR xylan Bacteroides xylanisolvens 27142817
Xylan degradation by the human gut Bacteroides xylanisolvens XB1A(T) involves two distinct gene clusters that are linked at the transcriptional level. BMC Genomics. 2016 May 4;17:326. doi: 10.1186/s12864-016-2680-8.
2016 May 4 degradation 8 3 GH67, GH13_14
PUL0402 Northern Blot, enzyme activity assay xylan, xylose Lactococcus lactis subsp. lactis IO-1 11282589
Genetic evidence for a defective xylan degradation pathway in Lactococcus lactis. Appl Environ Microbiol. 2001 Apr;67(4):1445-52. doi: 10.1128/AEM.67.4.1445-1452.2001.
2001 Apr degradation 6 1 GH43_11, GH43
PUL0406 high performance anion exchange chromatography beta-glucan Coprothermobacter proteolyticus 30315317
From proteins to polysaccharides: lifestyle and genetic evolution of Coprothermobacter proteolyticus. ISME J. 2019 Mar;13(3):603-617. doi: 10.1038/s41396-018-0290-y. Epub 2018 Oct 12.
2019 Mar degradation 20 3 GH16, GH3, GH18
PUL0411 enzyme activity assay xylan Prevotella bryantii 7487028
A xylan hydrolase gene cluster in Prevotella ruminicola B(1)4: sequence relationships, synergistic interactions, and oxygen sensitivity of a novel enzyme with exoxylanase and beta-(1,4)-xylosidase activities. Appl Environ Microbiol. 1995 Aug;61(8):2958-64. doi: 10.1128/aem.61.8.2958-2964.1995.
1995 Aug degradation 2 2 GH10, GH43_1
PUL0414 enzyme activity assay, thin layer chromatography xylan uncultured bacterium 35A20 30116044
Functional metagenomics reveals abundant polysaccharide-degrading gene clusters and cellobiose utilization pathways within gut microbiota of a wood-feeding higher termite. ISME J. 2019 Jan;13(1):104-117. doi: 10.1038/s41396-018-0255-1. Epub 2018 Aug 16.
2019 Jan degradation 28 7 GH10, GH10
PUL0415 microarray xylan, glucomannan, plant polysaccharide Bacteroides ovatus 22205877
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 4 2 GH20, GH20
PUL0456 microarray, RNA-Seq xylan Prevotella bryantii 20622018
Transcriptomic analyses of xylan degradation by Prevotella bryantii and insights into energy acquisition by xylanolytic bacteroidetes. J Biol Chem. 2010 Sep 24;285(39):30261-73. doi: 10.1074/jbc.M110.141788. Epub 2010 Jul 9.
2010 Sep 24 degradation 13 6 CE6, GH43_10, GH43, GH43_1, GH43, GH10, GH67
PUL0474 growth assay beta-glucan, xylan Flavobacterium johnsoniae 19717629
Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28.
2009 Nov degradation 9 5 GH30_1, GH30, GH30_1, GH30, GH3, GH30_3, GH3
PUL0477 growth assay beta-glucan Flavobacterium johnsoniae 19717629
Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28.
2009 Nov degradation 6 2 GH16, GH16
PUL0526 microarray, qPCR beta-glucan Bacteroides ovatus 22205877
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 6 2 GH30_3, GH30, GH3
PUL0528 microarray, qPCR beta-glucan Bacteroides ovatus 22205877
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 7 3 GH3, GH16, GH3
PUL0533 RNA-Seq xylan, arabinoxylan Bacteroides cellulosilyticus 23976882
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.
2013 degradation 9 3 GH115, GH10, GH30_8
PUL0537 RNA-Seq beta-glucan Bacteroides cellulosilyticus 23976882
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.
2013 degradation 7 3 GH3, GH3, GH157
PUL0542 substrate binding assay xylan Geobacillus stearothermophilus 10368143
The glucuronic acid utilization gene cluster from Bacillus stearothermophilus T-6. J Bacteriol. 1999 Jun;181(12):3695-704. doi: 10.1128/JB.181.12.3695-3704.1999.
1999 Jun degradation 30 6 CE4, GH52, GH10, GH67, GH39, GH43_11
PUL0543 RNA-Seq cellobiose, beta-glucan, glucosamine Bacteroides cellulosilyticus 23976882
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.
2013 degradation 6 3 GH30, GH30_3, GH2, CBM57, GH88
PUL0551 RNA-Seq cellobiose, beta-glucan, glucomannan, laminarin Bacteroides cellulosilyticus 23976882
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.
2013 degradation 6 2 GH16, GH3
PUL0553 RT-PCR, qPCR xylan Bacteroides xylanisolvens 27142817
Xylan degradation by the human gut Bacteroides xylanisolvens XB1A(T) involves two distinct gene clusters that are linked at the transcriptional level. BMC Genomics. 2016 May 4;17:326. doi: 10.1186/s12864-016-2680-8.
2016 May 4 degradation 21 13 GH43_10, GH43, CBM6, GH3, GH43, GH43_12, GH31, GH97, CBM66, CBM6, GH43, GH43_29, GH43, GH43_12, CBM4, CBM0, GH10, GH5_21, CE1, CE6, CBM6, GH43_29, GH95, GH115
PUL0599 liquid chromatography and mass spectrometry, differential gene expression xylan Clostridium cellulovorans 26020016
Elucidation of the recognition mechanisms for hemicellulose and pectin in Clostridium cellulovorans using intracellular quantitative proteome analysis. AMB Express. 2015 May 23;5:29. doi: 10.1186/s13568-015-0115-6. eCollection 2015.
2015 degradation 7 1 GH43, GH43_11
PUL0602 sequence homology analysis xylan Parageobacillus thermoglucosidasius 26442136
Complete genome sequence of Geobacillus thermoglucosidasius C56-YS93, a novel biomass degrader isolated from obsidian hot spring in Yellowstone National Park. Stand Genomic Sci. 2015 Oct 5;10:73. doi: 10.1186/s40793-015-0031-z. eCollection 2015.
2015 degradation 26 6 CBM22, GH10, GH39, GH67, GH10, GH52, CE4
PUL0610 enzyme activity assay, strcutural analysis xylan Rhodothermus marinus 31992772
Characterization and diversity of the complete set of GH family 3 enzymes from Rhodothermus marinus DSM 4253. Sci Rep. 2020 Jan 28;10(1):1329. doi: 10.1038/s41598-020-58015-5.
2020 Jan 28 degradation 14 6 GH43_15, CBM6, GH10, CBM4, 3.2.1.8, GH10, GH67, GH3, GH3
PUL0617 RNA-Seq xylan Prevotella sp. PINT 33113351
Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp. Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.
2020 Dec 9 degradation 14 6 GH67, GH10, GH43, GH43_1, GH43_35, GH43, GH5_21, GH5, GH10, CBM0, CBM4, 3.2.1.8
PUL0619 RNA-Seq xylan Prevotella sp. PROD 33113351
Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp. Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.
2020 Dec 9 degradation 5 1 GH35, CBM32
PUL0620 RNA-Seq xylan Prevotella sp. PROD 33113351
Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp. Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.
2020 Dec 9 degradation 10 2 GH128, GH51, GH43_19
PUL0622 RNA-Seq xylan Prevotella sp. PROD 33113351
Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp. Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.
2020 Dec 9 degradation 15 5 CBM35, CBM2, PL11_1, PL11, GH3, 3.2.1.55, CBM6, 3.2.1.37, GH43_7, CBM13, GH43, PL11_1, CBM35, CE12, PL11, CBM2, GH2
PUL0624 RNA-Seq xylan Prevotella sp. PMUR 33113351
Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp. Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.
2020 Dec 9 degradation 11 3 CBM13, GH43, GH43_24, GH43_19, GH51, GH128
PUL0625 RNA-Seq xylan Prevotella sp. PMUR 33113351
Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp. Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.
2020 Dec 9 degradation 18 9 GH43_12, GH43, GH115, GH43, GH43_10, GH97, CBM6, GH43_29, GH43, CBM22, CE1, CBM48, CBM51, CBM32, GH95, CBM13, GH115, GH30_8
PUL0630 enzyme activity assay, affinity gel electrophoresis xylan termite gut metagenome 33187992
Multimodularity of a GH10 Xylanase Found in the Termite Gut Metagenome. Appl Environ Microbiol. 2021 Jan 15;87(3):e01714-20. doi: 10.1128/AEM.01714-20. Print 2021 Jan 15.
2021 Jan 15 degradation 9 5 GH10, CBM4, CBM0, 3.2.1.8, GH115, GH11, CBM6, CBM36, CBM0, 3.2.1.8, GH10, CBM9, CBM22, GH43, GH43_1
PUL0646 recombinant protein expression, crystallization, affinity gel electrophoresis, isothermal titration calorimetry beta-glucan Bacteroides fluxus YIT 12057 33587952
Distinct protein architectures mediate species-specific beta-glucan binding and metabolism in the human gut microbiota. J Biol Chem. 2021 Jan-Jun;296:100415. doi: 10.1016/j.jbc.2021.100415. Epub 2021 Feb 13.
2021 Jan-Jun degradation 6 2 GH3, CBM6, GH158
PUL0669 clone, high performance anion exchange chromatography, enzymatic product analysis xylan Bacteroides eggerthii 1_2_48FAA 34480044
Characterization of a novel multidomain CE15-GH8 enzyme encoded by a polysaccharide utilization locus in the human gut bacterium Bacteroides eggerthii. Sci Rep. 2021 Sep 3;11(1):17662. doi: 10.1038/s41598-021-96659-z.
2021 Sep 3 degradation 24 12 CE15, GH8, GH95, GH43