Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0037 enzyme activity assay raffinose Streptococcus pneumoniae 31591266
Molecular analysis of an enigmatic Streptococcus pneumoniae virulence factor: The raffinose-family oligosaccharide utilization system. J Biol Chem. 2019 Nov 15;294(46):17197-17208. doi: 10.1074/jbc.RA119.010280. Epub 2019 Oct 7.
2019 Nov 15 degradation 7 2 GH13, GH13_18
PUL0038 enzyme activity assay, Southern Blot melibiose, galactose Thermus brockianus 10741834
The structure of the alpha-galactosidase gene loci in Thermus brockianus ITI360 and Thermus thermophilus TH125. Extremophiles. 2000 Feb;4(1):23-33. doi: 10.1007/s007920050004.
2000 Feb degradation 8 2 GH42, GH36
PUL0039 enzyme activity assay, Southern Blot melibiose, galactose Thermus thermophilus 10741834
The structure of the alpha-galactosidase gene loci in Thermus brockianus ITI360 and Thermus thermophilus TH125. Extremophiles. 2000 Feb;4(1):23-33. doi: 10.1007/s007920050004.
2000 Feb degradation 3 1 GH36
PUL0068 enzyme activity assay, electrophoretic mobility shift assay raffinose Escherichia coli 8277949
Role of two operators in regulating the plasmid-borne raf operon of Escherichia coli. Mol Gen Genet. 1994 Jan;242(1):90-9. doi: 10.1007/BF00277352.
1994 Jan degradation 4 2 GH36, GH32
PUL0082 electrophoretic mobility shift assay, enzyme activity assay melibiose, raffinose-oligosaccharide, stachyose Bacillus subtilis 31138628
The melREDCA Operon Encodes a Utilization System for the Raffinose Family of Oligosaccharides in Bacillus subtilis. J Bacteriol. 2019 Jul 10;201(15):e00109-19. doi: 10.1128/JB.00109-19. Print 2019 Aug 1.
2019 Aug 1 degradation 6 2 GH4
PUL0088 Southern Blot raffinose, melibiose, isomaltotriose Streptococcus mutans 8764489
The multiple-sugar metabolism (msm) gene cluster of Streptococcus mutans is transcribed as a single operon. FEMS Microbiol Lett. 1996 Jul 1;140(2-3):261-4. doi: 10.1016/0378-1097(96)00191-7.
1996 Jul 1 degradation 8 3 GH36, GH13_18, GH13
PUL0111 enzyme activity assay, Northern Blot, transport assay melibiose Escherichia coli 9642246
Conversion of temperature-sensitive to -resistant gene expression due to mutations in the promoter region of the melibiose operon in Escherichia coli. J Biol Chem. 1998 Jul 3;273(27):16860-4. doi: 10.1074/jbc.273.27.16860.
1998 Jul 3 degradation 3 1 GH4
PUL0122 enzyme activity assay alpha-galactoside, beta-galactoside, raffinose, melibiose Thermotoga maritima 9741105
Properties of an alpha-galactosidase, and structure of its gene galA, within an alpha-and beta-galactoside utilization gene cluster of the hyperthermophilic bacterium Thermotoga maritima. Syst Appl Microbiol. 1998 Mar;21(1):1-11. doi: 10.1016/s0723-2020(98)80002-7.
1998 Mar degradation 6 3 GH42, GH2, GH36
PUL0138 sequence homology analysis raffinose, melibiose Bifidobacterium animalis subsp. animalis 30306201
Staying alive: growth and survival of Bifidobacterium animalis subsp. animalis under in vitro and in vivo conditions. Appl Microbiol Biotechnol. 2018 Dec;102(24):10645-10663. doi: 10.1007/s00253-018-9413-7. Epub 2018 Oct 10.
2018 Dec degradation 7 2 GH36, GH36
PUL0176 RT-PCR, enzyme activity assay, enzymatic product analysis isomaltose Kribbella flavida 27302067
Two Novel Glycoside Hydrolases Responsible for the Catabolism of Cyclobis-(1-->6)-alpha-nigerosyl. J Biol Chem. 2016 Aug 5;291(32):16438-47. doi: 10.1074/jbc.M116.727305. Epub 2016 Jun 14.
2016 Aug 5 degradation 6 2 GH31, GH15
PUL0232 microarray, electrophoretic mobility shift assay raffinose Bifidobacterium breve 24705323
Transcription of two adjacent carbohydrate utilization gene clusters in Bifidobacterium breve UCC2003 is controlled by LacI- and repressor open reading frame kinase (ROK)-type regulators. Appl Environ Microbiol. 2014 Jun;80(12):3604-14. doi: 10.1128/AEM.00130-14.
2014 Jun degradation 6 1 GH36
PUL0276 RT-qPCR isomaltose, panose, raffinose, stachyose, melibiose Bifidobacterium animalis subsp. lactis 23663691
Transcriptional analysis of oligosaccharide utilization by Bifidobacterium lactis Bl-04. BMC Genomics. 2013 May 10;14:312. doi: 10.1186/1471-2164-14-312.
2013 May 10 degradation 9 2 GH36, GH36
PUL0374 microarray, qPCR melibiose Dickeya dadantii 19734309
Catabolism of raffinose, sucrose, and melibiose in Erwinia chrysanthemi 3937. J Bacteriol. 2009 Nov;191(22):6960-7. doi: 10.1128/JB.00594-09. Epub 2009 Sep 4.
2009 Nov degradation 3 1 GH36
PUL0486 RT-PCR, sugar utilization assay raffinose Streptococcus pneumoniae 10613841
Regulation of the alpha-galactosidase activity in Streptococcus pneumoniae: characterization of the raffinose utilization system. Genome Res. 1999 Dec;9(12):1189-97. doi: 10.1101/gr.9.12.1189.
1999 Dec degradation 8 2 GH13, GH13_18, GH36
PUL0575 microarray, growth assay, gene deletion mutant and growth assay raffinose Enterococcus faecium 20946531
A genetic element present on megaplasmids allows Enterococcus faecium to use raffinose as carbon source. Environ Microbiol. 2011 Feb;13(2):518-28. doi: 10.1111/j.1462-2920.2010.02355.x. Epub 2010 Oct 15.
2011 Feb degradation 11 2 GH13, GH13_18, GH13_31, GH13, GH36, GH4
PUL0584 microarray melibiose Lactococcus lactis 23530958
Genotype-phenotype matching analysis of 38 Lactococcus lactis strains using random forest methods. BMC Microbiol. 2013 Mar 26;13:68. doi: 10.1186/1471-2180-13-68.
2013 Mar 26 degradation 11 2 GH13, GH13_18, GH36