Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0017 qRT-PCR, Western Blot, isothermal titration calorimetry cellobiose Ruminiclostridium cellulolyticum 29093754
A seven-gene cluster in Ruminiclostridium cellulolyticum is essential for signalization, uptake and catabolism of the degradation products of cellulose hydrolysis. Biotechnol Biofuels. 2017 Oct 30;10:250. doi: 10.1186/s13068-017-0933-7. eCollection 2017.
2017 degradation 9 1 GH94
PUL0026 qPCR, Western Blot, RNA-Seq, enzyme activity assay ribose Bacteroides thetaiotaomicron 31901520
A Ribose-Scavenging System Confers Colonization Fitness on the Human Gut Symbiont Bacteroides thetaiotaomicron in a Diet-Specific Manner. Cell Host Microbe. 2020 Jan 8;27(1):79-92.e9. doi: 10.1016/j.chom.2019.11.009. Epub 2019 Dec 31.
2020 Jan 8 degradation 8 1 GH0, GH35
PUL0144 enzyme activity assay, Western Blot chitin Thermococcus kodakarensis 16199574
Characterization of a novel glucosamine-6-phosphate deaminase from a hyperthermophilic archaeon. J Bacteriol. 2005 Oct;187(20):7038-44. doi: 10.1128/JB.187.20.7038-7044.2005.
2005 Oct degradation 12 3 GH35, GH1, CBM2, GH18
PUL0216 Western Blot, enzyme activity assay, RT-PCR, microarray alginate Sphingomonas sp. 24816607
Alginate-dependent gene expression mechanism in Sphingomonas sp. strain A1. J Bacteriol. 2014 Jul;196(14):2691-700. doi: 10.1128/JB.01666-14. Epub 2014 May 9.
2014 Jul degradation 10 1 PL7, PL5, PL15_1
PUL0226 Western Blot xanthan Xanthomonas campestris 23089617
Xanthan chain length is modulated by increasing the availability of the polysaccharide copolymerase protein GumC and the outer membrane polysaccharide export protein GumB. Glycobiology. 2013 Feb;23(2):259-72. doi: 10.1093/glycob/cws146. Epub 2012 Oct 22.
2013 Feb biosynthesis 13 4 GT4, GT94, GT70, GT26
PUL0245 enzyme activity assay, gene deletion mutant and growth assay, Western Blot fucose Streptococcus pneumoniae 24333485
Structural and functional analysis of fucose-processing enzymes from Streptococcus pneumoniae. J Mol Biol. 2014 Apr 3;426(7):1469-82. doi: 10.1016/j.jmb.2013.12.006. Epub 2013 Dec 12.
2014 Apr 3 degradation 11 2 GH98, CBM47, GH95
PUL0246 enzyme activity assay, gene deletion mutant and growth assay, Western Blot fucose Streptococcus pneumoniae 24333485
Structural and functional analysis of fucose-processing enzymes from Streptococcus pneumoniae. J Mol Biol. 2014 Apr 3;426(7):1469-82. doi: 10.1016/j.jmb.2013.12.006. Epub 2013 Dec 12.
2014 Apr 3 degradation 11 4 GH98, CBM51, GH36, GH36, GH29
PUL0343 gene deletion mutant and growth assay, enzyme activity assay, Western Blot, isothermal titration calorimetry carboxymethylcellulose, xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 39 1 GH5, GH5_2
PUL0368 microarray, Western Blot human milk oligosaccharide Bifidobacterium longum subsp. infantis 19033196
The genome sequence of Bifidobacterium longum subsp. infantis reveals adaptations for milk utilization within the infant microbiome. Proc Natl Acad Sci U S A. 2008 Dec 2;105(48):18964-9. doi: 10.1073/pnas.0809584105. Epub 2008 Nov 24.
2008 Dec 2 degradation 30 4 GH2, GH95, GH29, GH33, GH20
PUL0434 SDS-PAGE, Western Blot mannooligosaccharide Clostridium cellulovorans 10613891
The engL gene cluster of Clostridium cellulovorans contains a gene for cellulosomal manA. J Bacteriol. 2000 Jan;182(1):244-7. doi: 10.1128/jb.182.1.244-247.2000.
2000 Jan degradation 8 2 CBM30, CBM4, GH9, GH9, GH5_17, CBM4, GH9, CBM4
PUL0579 microarray, qRT-PCR, Western Blot, immunoprecipitation N-acetylglucosamine Vibrio cholerae 21488982
Two gene clusters co-ordinate for a functional N-acetylglucosamine catabolic pathway in Vibrio cholerae. Mol Microbiol. 2011 Jun;80(6):1549-60. doi: 10.1111/j.1365-2958.2011.07664.x. Epub 2011 May 5.
2011 Jun degradation 3 1 CE9
PUL0580 microarray, qRT-PCR, Western Blot, immunoprecipitation N-acetylglucosamine Vibrio cholerae 21488982
Two gene clusters co-ordinate for a functional N-acetylglucosamine catabolic pathway in Vibrio cholerae. Mol Microbiol. 2011 Jun;80(6):1549-60. doi: 10.1111/j.1365-2958.2011.07664.x. Epub 2011 May 5.
2011 Jun degradation 3 1 CE9
PUL0589 Western Blot, enzyme activity assay, thin layer chromatography starch Streptococcus mutans 23930155
The malQ gene is essential for starch metabolism in Streptococcus mutans. J Oral Microbiol. 2013 Aug 6;5. pii: 21285. doi: 10.3402/jom.v5i0.21285. Print 2013.
2013 degradation 3 2 GT35, GH77