Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0151 sequence homology analysis, Northern Blot, RT-qPCR, electrophoretic mobility shift assay, clone and expression, gene deletion mutant and growth assay alginate Zobellia galactanivorans 30524390, 32585009
Evolutionary Evidence of Algal Polysaccharide Degradation Acquisition by Pseudoalteromonas carrageenovora 9(T) to Adapt to Macroalgal Niches. Regulation of alginate catabolism involves a GntR family repressor in the marine flavobacterium Zobellia galactanivorans DsijT. Front Microbiol. 2018 Nov 22;9:2740. doi: 10.3389/fmicb.2018.02740. eCollection 2018. Nucleic Acids Res. 2020 Aug 20;48(14):7786-7800. doi: 10.1093/nar/gkaa533.
2018,2020 Aug 20 degradation 12 2 PL7, PL17_2, PL17
PUL0313 microarray, Northern Blot, RT-qPCR, electrophoretic mobility shift assay, clone and expression, gene deletion mutant and growth assay alginate Zobellia galactanivorans 28983288, 32585009
Gene Expression Analysis of Zobellia galactanivorans during the Degradation of Algal Polysaccharides Reveals both Substrate-Specific and Shared Transcriptome-Wide Responses. Regulation of alginate catabolism involves a GntR family repressor in the marine flavobacterium Zobellia galactanivorans DsijT. Front Microbiol. 2017 Sep 21;8:1808. doi: 10.3389/fmicb.2017.01808. eCollection 2017. Nucleic Acids Res. 2020 Aug 20;48(14):7786-7800. doi: 10.1093/nar/gkaa533.
2017,2020 Aug 20 degradation 3 3 PL6, PL6_1, PL7_5, PL7, PL6, PL6_1
PUL0353 microarray, enzyme activity assay, strcutural analysis, clone and expression arabinan, xylan, levan, pectin, rhamnogalacturonan Bacteroides thetaiotaomicron 16968696, 32060313
Functional genomic and metabolic studies of the adaptations of a prominent adult human gut symbiont, Bacteroides thetaiotaomicron, to the suckling period. Structural basis of mammalian high-mannose N-glycan processing by human gut Bacteroides. J Biol Chem. 2006 Nov 24;281(47):36269-79. doi: 10.1074/jbc.M606509200. Epub 2006 Sep 12. Nat Commun. 2020 Feb 14;11(1):899. doi: 10.1038/s41467-020-14754-7.
2006 Nov 24,2020 Feb 14 degradation 11 3 GH0, GH92, GH92
PUL0412 clone and expression, enzyme activity assay polygalacturonic acid Ralstonia solanacearum 12795379
Characterization of a Ralstonia solanacearum operon required for polygalacturonate degradation and uptake of galacturonic acid. Mol Plant Microbe Interact. 2003 Jun;16(6):536-44. doi: 10.1094/MPMI.2003.16.6.536.
2003 Jun degradation 2 1 GH28
PUL0423 clone and expression, enzyme activity assay cellobiose Thermotoga neapolitana 10960102
Cloning and characterization of the glucooligosaccharide catabolic pathway beta-glucan glucohydrolase and cellobiose phosphorylase in the marine hyperthermophile Thermotoga neapolitana. J Bacteriol. 2000 Sep;182(18):5172-9. doi: 10.1128/jb.182.18.5172-5179.2000.
2000 Sep degradation 3 2 GH94
PUL0455 clone and expression, genes induced in presence of substrate, enzyme activity assay sucrose Bifidobacterium animalis 12513973
Induction of sucrose utilization genes from Bifidobacterium lactis by sucrose and raffinose. Appl Environ Microbiol. 2003 Jan;69(1):24-32. doi: 10.1128/aem.69.1.24-32.2003.
2003 Jan degradation 3 1 GH13, GH13_18
PUL0466 clone and expression, enzyme activity assay, Northern Blot arabinan Bacillus subtilis 14973026
Transcriptional regulation of genes encoding arabinan-degrading enzymes in Bacillus subtilis. J Bacteriol. 2004 Mar;186(5):1287-96. doi: 10.1128/jb.186.5.1287-1296.2004.
2004 Mar degradation 9 1 GH51
PUL0475 clone and expression, gene deletion mutant and growth assay cellobiose, cellotriose Streptomyces reticuli 10347054
Characterization of the binding protein-dependent cellobiose and cellotriose transport system of the cellulose degrader Streptomyces reticuli. Appl Environ Microbiol. 1999 Jun;65(6):2636-43. doi: 10.1128/AEM.65.6.2636-2643.1999.
1999 Jun degradation 7 1 GH18, CBM2
PUL0497 clone and expression, enzyme activity assay chitin Pseudoalteromonas piscicida 11772635
Identification and characterization of the gene cluster involved in chitin degradation in a marine bacterium, Alteromonas sp. strain O-7. Appl Environ Microbiol. 2002 Jan;68(1):263-70. doi: 10.1128/aem.68.1.263-270.2002.
2002 Jan degradation 3 2 CBM5, GH18, CBM5, AA10, CBM5, GH18
PUL0508 clone and expression, enzyme activity assay xylobiose, xylotriose Streptomyces thermoviolaceus 14761997
Molecular characterization of a high-affinity xylobiose transporter of Streptomyces thermoviolaceus OPC-520 and its transcriptional regulation. J Bacteriol. 2004 Feb;186(4):1029-37. doi: 10.1128/jb.186.4.1029-1037.2004.
2004 Feb degradation 5 2 GH3
PUL0520 clone and expression, enzyme activity assay xylobiose, xylodextrin Klebsiella oxytoca 14532050
Cloning, characterization, and functional expression of the Klebsiella oxytoca xylodextrin utilization operon (xynTB) in Escherichia coli. Appl Environ Microbiol. 2003 Oct;69(10):5957-67. doi: 10.1128/aem.69.10.5957-5967.2003.
2003 Oct degradation 2 1 GH43, GH43_11
PUL0531 clone and expression, enzyme activity assay chitobiose Serratia marcescens 12618440
Uptake of N,N'-diacetylchitobiose [(GlcNAc)2] via the phosphotransferase system is essential for chitinase production by Serratia marcescens 2170. J Bacteriol. 2003 Mar;185(6):1776-82. doi: 10.1128/jb.185.6.1776-1782.2003.
2003 Mar degradation 5 1 GH1
PUL0561 clone and expression, enzyme activity assay alpha-galactoside Lactobacillus plantarum 12406739
Characterization of the melA locus for alpha-galactosidase in Lactobacillus plantarum. Appl Environ Microbiol. 2002 Nov;68(11):5464-71. doi: 10.1128/aem.68.11.5464-5471.2002.
2002 Nov degradation 5 2 GH36, GH2
PUL0567 clone and expression, enzyme activity assay chitin Pseudoalteromonas sp. S9 10220172
Multiple genes involved in chitin degradation from the marine bacterium Pseudoalteromonas sp. strain S91. Microbiology (Reading). 1999 Apr;145 ( Pt 4):925-934. doi: 10.1099/13500872-145-4-925.
1999 Apr degradation 3 3 CBM5, GH18, AA10, CBM5, CBM5, GH18
PUL0568 clone and expression, enzyme activity assay, Northern Blot sucrose Clostridium beijerinckii 10411273
The genes controlling sucrose utilization in Clostridium beijerinckii NCIMB 8052 constitute an operon. Microbiology (Reading). 1999 Jun;145 ( Pt 6):1461-1472. doi: 10.1099/13500872-145-6-1461.
1999 Jun degradation 4 1 GH32
PUL0569 clone and expression, enzyme activity assay, Northern Blot levan Bacillus subtilis 11739774
yveB, Encoding endolevanase LevB, is part of the sacB-yveB-yveA levansucrase tricistronic operon in Bacillus subtilis. Microbiology (Reading). 2001 Dec;147(Pt 12):3413-9. doi: 10.1099/00221287-147-12-3413.
2001 Dec degradation 3 1 GH68, GH32
PUL0570 clone and expression, enzyme activity assay cellobiose Corynebacterium glutamicum 12777497
A single V317A or V317M substitution in Enzyme II of a newly identified beta-glucoside phosphotransferase and utilization system of Corynebacterium glutamicum R extends its specificity towards cellobiose. Microbiology (Reading). 2003 Jun;149(Pt 6):1569-1580. doi: 10.1099/mic.0.26053-0.
2003 Jun degradation 3 1 GH1
PUL0606 enzymy activity assay, clone and expression beta-galactooligosaccharide Bifidobacterium breve UCC2003 32385941
Biochemical analysis of cross-feeding behaviour between two common gut commensals when cultivated on plant-derived arabinogalactan. Microb Biotechnol. 2020 Nov;13(6):1733-1747. doi: 10.1111/1751-7915.13577. Epub 2020 May 9.
2020 Nov degradation 3 1 GH2
PUL0607 enzymy activity assay, clone and expression, liquid chromatography and mass spectrometry porphyran Wenyingzhuangia fucanilytica strain CZ1127 32520542
Characterization of a Novel Porphyranase Accommodating Methyl-galactoses at Its Subsites. J Agric Food Chem. 2020 Jul 1;68(26):7032-7039. doi: 10.1021/acs.jafc.0c02404. Epub 2020 Jun 22.
2020 Jul 1 degradation 22 8 GH2, CBM67, CBM51, GH141, PL0, GH105, GH154, GH16_11, GH16, GH117, GH16_11, GH16, GH16_14, GH29
PUL0608 enzymy activity assay, clone and expression, liquid chromatography and mass spectrometry, thin layer chromatography, MALDI-TOF/MS human milk oligosaccharide Roseburia hominis DSM 16839 32620774
Butyrate producing colonic Clostridiales metabolise human milk oligosaccharides and cross feed on mucin via conserved pathways. Nat Commun. 2020 Jul 3;11(1):3285. doi: 10.1038/s41467-020-17075-x.
2020 Jul 3 degradation 9 2 GH136, GH112
PUL0609 enzymy activity assay, clone and expression, liquid chromatography and mass spectrometry, thin layer chromatography, MALDI-TOF/MS human milk oligosaccharide Roseburia inulinivorans DSM 16841 32620774
Butyrate producing colonic Clostridiales metabolise human milk oligosaccharides and cross feed on mucin via conserved pathways. Nat Commun. 2020 Jul 3;11(1):3285. doi: 10.1038/s41467-020-17075-x.
2020 Jul 3 degradation 11 4 GH112, GH95, GH95, GH136