Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0017 qRT-PCR, Western Blot, isothermal titration calorimetry cellobiose Ruminiclostridium cellulolyticum 29093754
A seven-gene cluster in Ruminiclostridium cellulolyticum is essential for signalization, uptake and catabolism of the degradation products of cellulose hydrolysis. Biotechnol Biofuels. 2017 Oct 30;10:250. doi: 10.1186/s13068-017-0933-7. eCollection 2017.
2017 degradation 9 1 GH94
PUL0044 qRT-PCR, enzyme activity assay arabinoxylan Bacteroides ovatus 26112186, 32266006
Glycan complexity dictates microbial resource allocation in the large intestine. Multimodular fused acetyl-feruloyl esterases from soil and gut Bacteroidetes improve xylanase depolymerization of recalcitrant biomass. Nat Commun. 2015 Jun 26;6:7481. doi: 10.1038/ncomms8481. Biotechnol Biofuels. 2020 Mar 31;13:60. doi: 10.1186/s13068-020-01698-9. eCollection 2020.
2015 Jun 26,2020 degradation 33 16 GH43, GH43_10, GH3, GH43_12, GH43, GH31, GH97, CBM6, GH43, GH43_29, GH43_12, GH43, GH30_8, CBM35, GH98, GH115, CE6, CE1, CBM6, GH43, GH43_29, GH95, GH30, GH115
PUL0045 qRT-PCR arabinoxylan Bacteroides ovatus 26112186
Glycan complexity dictates microbial resource allocation in the large intestine. Nat Commun. 2015 Jun 26;6:7481. doi: 10.1038/ncomms8481.
2015 Jun 26 degradation 11 5 GH67, GH43_1, GH43, GH10, CBM4, GH10
PUL0081 qRT-PCR, microarray oligogalacturonide Vibrio parahaemolyticus 31133029
Carbohydrate metabolic systems present on genomic islands are lost and gained in Vibrio parahaemolyticus. BMC Microbiol. 2019 May 27;19(1):112. doi: 10.1186/s12866-019-1487-6.
2019 May 27 degradation 13 2 PL9_1, PL22_1, PL22
PUL0118 qRT-PCR, affinity gel electrophoresis, isothermal titration calorimetry laminarin, beta-glucan Bacteroides uniformis 32265336
Synergy between Cell Surface Glycosidases and Glycan-Binding Proteins Dictates the Utilization of Specific Beta(1,3)-Glucans by Human Gut Bacteroides. mBio. 2020 Apr 7;11(2). pii: mBio.00095-20. doi: 10.1128/mBio.00095-20.
2020 Apr 7 degradation 7 3 CBM6, GH3, GH158, GH16
PUL0169 enzyme activity assay, qRT-PCR arabinofuranooligosaccharide Xanthomonas euvesicatoria 30092047
Functional characterization of unique enzymes in Xanthomonas euvesicatoria related to degradation of arabinofurano-oligosaccharides on hydroxyproline-rich glycoproteins. PLoS One. 2018 Aug 9;13(8):e0201982. doi: 10.1371/journal.pone.0201982. eCollection 2018.
2018 degradation 9 3 GH146, GH43, GH43_29, GH121
PUL0171 qRT-PCR, RNA-Seq N-glycan Bacteroides fragilis 27353652
cis-Encoded Small RNAs, a Conserved Mechanism for Repression of Polysaccharide Utilization in Bacteroides. J Bacteriol. 2016 Aug 25;198(18):2410-8. doi: 10.1128/JB.00381-16. Print 2016 Sep 15.
2016 Sep 15 degradation 7 1 GH18
PUL0187 qRT-PCR, enzyme activity assay beta-glucan Paenibacillus sp. JDR-2 26746717
A 1,3-1,4-beta-Glucan Utilization Regulon in Paenibacillus sp. Strain JDR-2. Appl Environ Microbiol. 2016 Jan 8;82(6):1789-1798. doi: 10.1128/AEM.03526-15.
2016 Jan 8 degradation 7 2 CBM4, CBM54, CBM6, GH16, GH16
PUL0212 qRT-PCR galactooligosaccharide Bifidobacterium adolescentis 25483279
Diverse galactooligosaccharides consumption by bifidobacteria: implications of beta-galactosidase--LacS operon. Biosci Biotechnol Biochem. 2015;79(4):664-72. doi: 10.1080/09168451.2014.987204. Epub 2014 Dec 6.
2015 degradation 8 1 GH2
PUL0213 qRT-PCR galactooligosaccharide Bifidobacterium adolescentis 25483279
Diverse galactooligosaccharides consumption by bifidobacteria: implications of beta-galactosidase--LacS operon. Biosci Biotechnol Biochem. 2015;79(4):664-72. doi: 10.1080/09168451.2014.987204. Epub 2014 Dec 6.
2015 degradation 4 1 GH2
PUL0214 qRT-PCR galactooligosaccharide Bifidobacterium adolescentis 25483279
Diverse galactooligosaccharides consumption by bifidobacteria: implications of beta-galactosidase--LacS operon. Biosci Biotechnol Biochem. 2015;79(4):664-72. doi: 10.1080/09168451.2014.987204. Epub 2014 Dec 6.
2015 degradation 9 2 GH42, GH35
PUL0224 RT-PCR, qRT-PCR cellulose Ruminiclostridium cellulolyticum 23418511
A two-component system (XydS/R) controls the expression of genes encoding CBM6-containing proteins in response to straw in Clostridium cellulolyticum. PLoS One. 2013;8(2):e56063. doi: 10.1371/journal.pone.0056063. Epub 2013 Feb 13.
2013 degradation 16 14 GH43_16, GH43, CBM6, GH10, CBM6, GH43, GH43_29, CBM6, CBM6, CE1, GH43_10, CBM6, GH62, CBM6, GH43, GH43_29, CBM6, GH146, CBM22, GH27, CBM6, GH59, CBM6, GH2, CBM6, GH62, CBM6, CE6, CBM32, GH95, CBM6, GH30_8, CBM6
PUL0228 qRT-PCR, enzyme activity assay, high performance anion exchange chromatography arabinan Bacteroides thetaiotaomicron 22686399, 21339299
Prioritization of a plant polysaccharide over a mucus carbohydrate is enforced by a Bacteroides hybrid two-component system. The structure and function of an arabinan-specific alpha-1,2-arabinofuranosidase identified from screening the activities of bacterial GH43 glycoside hydrolases. Mol Microbiol. 2012 Aug;85(3):478-91. doi: 10.1111/j.1365-2958.2012.08123.x. Epub 2012 Jul 5. J Biol Chem. 2011 Apr 29;286(17):15483-95. doi: 10.1074/jbc.M110.215962. Epub 2011 Feb 21.
2012 Aug,2011 Apr 29 degradation 10 4 GH43, GH43_4, GH43_4, GH51, GH43_29
PUL0277 gene deletion mutant and growth assay, qRT-PCR fructooligosaccharide Streptococcus pneumoniae 23264576
The ABC transporter encoded at the pneumococcal fructooligosaccharide utilization locus determines the ability to utilize long- and short-chain fructooligosaccharides. J Bacteriol. 2013 Mar;195(5):1031-41. doi: 10.1128/JB.01560-12. Epub 2012 Dec 21.
2013 Mar degradation 5 1 GH32
PUL0278 gene deletion mutant and growth assay, qRT-PCR fructooligosaccharide Streptococcus pneumoniae 23264576
The ABC transporter encoded at the pneumococcal fructooligosaccharide utilization locus determines the ability to utilize long- and short-chain fructooligosaccharides. J Bacteriol. 2013 Mar;195(5):1031-41. doi: 10.1128/JB.01560-12. Epub 2012 Dec 21.
2013 Mar degradation 6 1 GH32
PUL0279 gene deletion mutant and growth assay, qRT-PCR fructooligosaccharide Streptococcus pneumoniae 23264576
The ABC transporter encoded at the pneumococcal fructooligosaccharide utilization locus determines the ability to utilize long- and short-chain fructooligosaccharides. J Bacteriol. 2013 Mar;195(5):1031-41. doi: 10.1128/JB.01560-12. Epub 2012 Dec 21.
2013 Mar degradation 6 1 GH32
PUL0280 microarray, qRT-PCR capsule polysaccharide Streptococcus suis 23226442
HP0197 contributes to CPS synthesis and the virulence of Streptococcus suis via CcpA. PLoS One. 2012;7(11):e50987. doi: 10.1371/journal.pone.0050987. Epub 2012 Nov 30.
2012 biosynthesis 15 5 GT4, GT4, GT2, GT2, GT2
PUL0345 qRT-PCR, enzyme activity assay xylan Bacteroides intestinalis 27681607
Bacteroides intestinalis DSM 17393, a member of the human colonic microbiome, upregulates multiple endoxylanases during growth on xylan. Sci Rep. 2016 Sep 29;6:34360. doi: 10.1038/srep34360.
2016 Sep 29 degradation 31 13 CBM4, GH10, GH35, GH43_12, GH43, GH10, GH43_1, GH43, GH67, GH8, CE6, GH95, CE0, CE1, GH5, GH5_21, GH10, CBM0, GH115
PUL0387 gene deletion mutant and growth assay, qRT-PCR, GlcNAc phosphorylation assays N-acetylglucosamine Xanthomonas campestris pv. campestris 20081036
Identification and regulation of the N-acetylglucosamine utilization pathway of the plant pathogenic bacterium Xanthomonas campestris pv. campestris. J Bacteriol. 2010 Mar;192(6):1487-97. doi: 10.1128/JB.01418-09. Epub 2010 Jan 15.
2010 Mar degradation 7 1 CE9
PUL0555 gene deletion mutant and growth assay, qRT-PCR, microarray, enzyme activity assay N-glycan Bacteroides fragilis 25139987
Efficient utilization of complex N-linked glycans is a selective advantage for Bacteroides fragilis in extraintestinal infections. Proc Natl Acad Sci U S A. 2014 Sep 2;111(35):12901-6. doi: 10.1073/pnas.1407344111. Epub 2014 Aug 19.
2014 Sep 2 degradation 9 5 GH154, GH2, GH20, GH88, PL0, PL33_1
PUL0556 gene deletion mutant and growth assay, qRT-PCR, microarray, enzyme activity assay N-glycan Bacteroides fragilis 25139987
Efficient utilization of complex N-linked glycans is a selective advantage for Bacteroides fragilis in extraintestinal infections. Proc Natl Acad Sci U S A. 2014 Sep 2;111(35):12901-6. doi: 10.1073/pnas.1407344111. Epub 2014 Aug 19.
2014 Sep 2 degradation 7 1 GH18
PUL0573 enzyme activity assay, electrophoretic mobility shift assay, RT-PCR, qRT-PCR cellobiose, cellulose, cellooligosaccharide Streptomyces griseus 19648249
CebR as a master regulator for cellulose/cellooligosaccharide catabolism affects morphological development in Streptomyces griseus. J Bacteriol. 2009 Oct;191(19):5930-40. doi: 10.1128/JB.00703-09. Epub 2009 Jul 31.
2009 Oct degradation 5 1 GH1
PUL0578 qRT-PCR, enzyme activity assay, electrophoretic mobility shift assay cellodextrin Bifidobacterium breve 21216899
Cellodextrin utilization by bifidobacterium breve UCC2003. Appl Environ Microbiol. 2011 Mar;77(5):1681-90. doi: 10.1128/AEM.01786-10. Epub 2011 Jan 7.
2011 Mar degradation 5 1 GH1
PUL0579 microarray, qRT-PCR, Western Blot, immunoprecipitation N-acetylglucosamine Vibrio cholerae 21488982
Two gene clusters co-ordinate for a functional N-acetylglucosamine catabolic pathway in Vibrio cholerae. Mol Microbiol. 2011 Jun;80(6):1549-60. doi: 10.1111/j.1365-2958.2011.07664.x. Epub 2011 May 5.
2011 Jun degradation 3 1 CE9
PUL0580 microarray, qRT-PCR, Western Blot, immunoprecipitation N-acetylglucosamine Vibrio cholerae 21488982
Two gene clusters co-ordinate for a functional N-acetylglucosamine catabolic pathway in Vibrio cholerae. Mol Microbiol. 2011 Jun;80(6):1549-60. doi: 10.1111/j.1365-2958.2011.07664.x. Epub 2011 May 5.
2011 Jun degradation 3 1 CE9
PUL0590 qRT-PCR, gene deletion mutant and growth assay, microarray maltose, maltodextrin Enterococcus faecium 23951303
A LacI-family regulator activates maltodextrin metabolism of Enterococcus faecium. PLoS One. 2013 Aug 7;8(8):e72285. doi: 10.1371/journal.pone.0072285. eCollection 2013.
2013 degradation 5 1 CBM34, GH13_20
PUL0592 qRT-PCR methylglucuronoarabinoxylan Paenibacillus sp. JDR-2 25063665
GH51 arabinofuranosidase and its role in the methylglucuronoarabinoxylan utilization system in Paenibacillus sp. strain JDR-2. Appl Environ Microbiol. 2014 Oct;80(19):6114-25. doi: 10.1128/AEM.01684-14. Epub 2014 Jul 25.
2014 Oct degradation 8 3 GH67, GH10, GH43_12
PUL0594 qRT-PCR methylglucuronoarabinoxylan Paenibacillus sp. JDR-2 25063665
GH51 arabinofuranosidase and its role in the methylglucuronoarabinoxylan utilization system in Paenibacillus sp. strain JDR-2. Appl Environ Microbiol. 2014 Oct;80(19):6114-25. doi: 10.1128/AEM.01684-14. Epub 2014 Jul 25.
2014 Oct degradation 4 1 GH51
PUL0603 microarray, qRT-PCR, culureing methods galactomannooligosaccharide Lactobacillus plantarum WCFS1 31703861
Transcriptional analysis of galactomannooligosaccharides utilization by Lactobacillus plantarum WCFS1. Food Microbiol. 2020 Apr;86:103336. doi: 10.1016/j.fm.2019.103336. Epub 2019 Sep 14.
2020 Apr degradation 3 1 GH1
PUL0604 microarray, qRT-PCR, culureing methods galactomannooligosaccharide Lactobacillus plantarum WCFS1 31703861
Transcriptional analysis of galactomannooligosaccharides utilization by Lactobacillus plantarum WCFS1. Food Microbiol. 2020 Apr;86:103336. doi: 10.1016/j.fm.2019.103336. Epub 2019 Sep 14.
2020 Apr degradation 6 2 GH32, GH13_31, GH13