Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0018 rapid plate method growth assay, gene deletion mutant and growth assay, RT-PCR, enzyme activity assay glycosaminoglycan Streptococcus pneumoniae 22311922
Streptococcus pneumoniae can utilize multiple sources of hyaluronic acid for growth. Infect Immun. 2012 Apr;80(4):1390-8. doi: 10.1128/IAI.05756-11. Epub 2012 Feb 6.
2012 Apr degradation 12 3 CBM70, PL8_1, PL8, GH88, PL12_1
PUL0022 RT-PCR, gene deletion mutant and growth assay, enzyme activity assay cellobiose Bacillus coagulans 30519284
Simultaneous consumption of cellobiose and xylose by Bacillus coagulans to circumvent glucose repression and identification of its cellobiose-assimilating operons. Biotechnol Biofuels. 2018 Dec 1;11:320. doi: 10.1186/s13068-018-1323-5. eCollection 2018.
2018 degradation 6 1 GH1
PUL0023 RT-PCR, gene deletion mutant and growth assay, enzyme activity assay cellobiose Bacillus coagulans 30519284
Simultaneous consumption of cellobiose and xylose by Bacillus coagulans to circumvent glucose repression and identification of its cellobiose-assimilating operons. Biotechnol Biofuels. 2018 Dec 1;11:320. doi: 10.1186/s13068-018-1323-5. eCollection 2018.
2018 degradation 5 1 GH1
PUL0027 Northern Blot, gene deletion mutant and growth assay alginate Azotobacter vinelandii 10352233
Transcriptional organization of the Azotobacter vinelandii algGXLVIFA genes: characterization of algF mutants. Gene. 1999 May 31;232(2):217-22. doi: 10.1016/s0378-1119(99)00119-5.
1999 May 31 biosynthesis 5 1 PL5, PL5_1
PUL0030 isothermal calorimetric titration, gene deletion mutant and growth assay, enzyme activity assay mannan Bacillus sp. N16-5 26978267, 30351049
A Novel Manno-Oligosaccharide Binding Protein Identified in Alkaliphilic Bacillus sp. N16-5 Is Involved in Mannan Utilization. Galactomannan Degrading Enzymes from the Mannan Utilization Gene Cluster of Alkaliphilic Bacillus sp. N16-5 and Their Synergy on Galactomannan Degradation. PLoS One. 2016 Mar 15;11(3):e0150059. doi: 10.1371/journal.pone.0150059. eCollection 2016. J Agric Food Chem. 2018 Oct 24;66(42):11055-11063. doi: 10.1021/acs.jafc.8b03878. Epub 2018 Oct 15.
2016,2018 Oct 24 degradation 12 7 GH130, GH130, GH27, CE7
PUL0151 sequence homology analysis, Northern Blot, RT-qPCR, electrophoretic mobility shift assay, clone and expression, gene deletion mutant and growth assay alginate Zobellia galactanivorans 30524390, 32585009
Evolutionary Evidence of Algal Polysaccharide Degradation Acquisition by Pseudoalteromonas carrageenovora 9(T) to Adapt to Macroalgal Niches. Regulation of alginate catabolism involves a GntR family repressor in the marine flavobacterium Zobellia galactanivorans DsijT. Front Microbiol. 2018 Nov 22;9:2740. doi: 10.3389/fmicb.2018.02740. eCollection 2018. Nucleic Acids Res. 2020 Aug 20;48(14):7786-7800. doi: 10.1093/nar/gkaa533.
2018,2020 Aug 20 degradation 12 2 PL7, PL17_2, PL17
PUL0168 gene deletion mutant and growth assay galactose Lactococcus lactis subsp. lactis 30099846
GlaR (YugA)-a novel RpiR-family transcription activator of the Leloir pathway of galactose utilization in Lactococcus lactis IL1403. Microbiologyopen. 2019 May;8(5):e00714. doi: 10.1002/mbo3.714. Epub 2018 Aug 11.
2019 May degradation 9 1 GH2
PUL0172 gene deletion mutant and growth assay, NMR, sugar utilization assay O-antigen Franconibacter pulveris 27166227
O antigen of FranconibacterpulverisG3872 (O1) is a 4-deoxy-d-arabino-hexose-containing polysaccharide synthesized by the ABC-transporter-dependent pathway. Microbiology (Reading). 2016 Jul;162(7):1103-1113. doi: 10.1099/mic.0.000307. Epub 2016 May 10.
2016 Jul biosynthesis 9 3 GT2
PUL0185 isothermal calorimetric titration, gene deletion mutant and growth assay mannan Bacillus sp. N16-5 26978267
A Novel Manno-Oligosaccharide Binding Protein Identified in Alkaliphilic Bacillus sp. N16-5 Is Involved in Mannan Utilization. PLoS One. 2016 Mar 15;11(3):e0150059. doi: 10.1371/journal.pone.0150059. eCollection 2016.
2016 degradation 12 6 GH130, GH130, GH27, CE7
PUL0186 gene deletion mutant and growth assay cellobiose Streptococcus pneumoniae 17028271
The two-component regulatory system TCS08 is involved in cellobiose metabolism of Streptococcus pneumoniae R6. J Bacteriol. 2007 Feb;189(4):1342-50. doi: 10.1128/JB.01170-06. Epub 2006 Oct 6.
2007 Feb degradation 7 1 GH1
PUL0194 enzyme activity assay, gene deletion mutant and growth assay N-glycan Streptococcus pneumoniae 28056108
Molecular Characterization of N-glycan Degradation and Transport in Streptococcus pneumoniae and Its Contribution to Virulence. PLoS Pathog. 2017 Jan 5;13(1):e1006090. doi: 10.1371/journal.ppat.1006090. eCollection 2017 Jan.
2017 Jan degradation 6 5 GH20, GH38, GH125, GH92, GH29
PUL0197 gene deletion mutant and growth assay maltose Streptococcus mutans 17233733
Overlapping substrate specificity for sucrose and maltose of two binding protein-dependent sugar uptake systems in Streptococcus mutans. FEMS Microbiol Lett. 2007 Jan;266(2):218-23. doi: 10.1111/j.1574-6968.2006.00522.x.
2007 Jan degradation 7 2 GT35, GH77
PUL0206 gene deletion mutant and growth assay mucin Capnocytophaga canimorsus 25736888
Glycan-foraging systems reveal the adaptation of Capnocytophaga canimorsus to the dog mouth. mBio. 2015 Mar 3;6(2):e02507. doi: 10.1128/mBio.02507-14.
2015 Mar 3 degradation 8 1 GH2
PUL0209 enzyme activity assay, gene deletion mutant and growth assay galactan Dickeya dadantii 17644603
Characterization of the Erwinia chrysanthemi Gan locus, involved in galactan catabolism. J Bacteriol. 2007 Oct;189(19):7053-61. doi: 10.1128/JB.00845-07. Epub 2007 Jul 20.
2007 Oct degradation 9 2 GH42, GH53
PUL0211 enzyme activity assay, gene deletion mutant and growth assay, thin layer chromatography N-glycan Xanthomonas campestris pv. campestris 25586188, 25205095
The N-Glycan cluster from Xanthomonas campestris pv. campestris: a toolbox for sequential plant N-glycan processing. The plant pathogen Xanthomonas campestris pv. campestris exploits N-acetylglucosamine during infection. J Biol Chem. 2015 Mar 6;290(10):6022-36. doi: 10.1074/jbc.M114.624593. Epub 2015 Jan 13. mBio. 2014 Sep 9;5(5):e01527-14. doi: 10.1128/mBio.01527-14.
2015 Mar 6,2014 Sep 9 degradation 9 6 GH29, GH18, GH20, GH2, GH3, GH125, GH92, GH35
PUL0237 gene deletion mutant and growth assay exopolysaccharide Pseudomonas alkylphenolica 24493568
An alginate-like exopolysaccharide biosynthesis gene cluster involved in biofilm aerial structure formation by Pseudomonas alkylphenolia. Appl Microbiol Biotechnol. 2014 May;98(9):4137-48. doi: 10.1007/s00253-014-5529-6. Epub 2014 Feb 4.
2014 May biosynthesis 12 2 PL5
PUL0244 gene deletion mutant and growth assay, complementation study, carbohydrate binding assay sialic acid Tannerella forsythia 24351045
Structural and functional characterization of NanU, a novel high-affinity sialic acid-inducible binding protein of oral and gut-dwelling Bacteroidetes species. Biochem J. 2014 Mar 15;458(3):499-511. doi: 10.1042/BJ20131415.
2014 Mar 15 degradation 9 2 GH20, GH33
PUL0245 enzyme activity assay, gene deletion mutant and growth assay, Western Blot fucose Streptococcus pneumoniae 24333485
Structural and functional analysis of fucose-processing enzymes from Streptococcus pneumoniae. J Mol Biol. 2014 Apr 3;426(7):1469-82. doi: 10.1016/j.jmb.2013.12.006. Epub 2013 Dec 12.
2014 Apr 3 degradation 11 2 GH98, CBM47, GH95
PUL0246 enzyme activity assay, gene deletion mutant and growth assay, Western Blot fucose Streptococcus pneumoniae 24333485
Structural and functional analysis of fucose-processing enzymes from Streptococcus pneumoniae. J Mol Biol. 2014 Apr 3;426(7):1469-82. doi: 10.1016/j.jmb.2013.12.006. Epub 2013 Dec 12.
2014 Apr 3 degradation 11 4 GH98, CBM51, GH36, GH36, GH29
PUL0247 primer extension analysis, gene deletion mutant and growth assay capsule polysaccharide Vibrio vulnificus 24102883, 16484211
Role of capsular polysaccharide (CPS) in biofilm formation and regulation of CPS production by quorum-sensing in Vibrio vulnificus. Genetic variation in the Vibrio vulnificus group 1 capsular polysaccharide operon. Mol Microbiol. 2013 Nov;90(4):841-57. doi: 10.1111/mmi.12401. Epub 2013 Oct 10. J Bacteriol. 2006 Mar;188(5):1987-98. doi: 10.1128/JB.188.5.1987-1998.2006.
2013 Nov,2006 Mar degradation 18 4 GT4, GT4
PUL0265 enzyme activity assay, gene deletion mutant and growth assay maltose, maltotriose Staphylococcus xylosus 7730272
Characterization of a genetic locus essential for maltose-maltotriose utilization in Staphylococcus xylosus. J Bacteriol. 1995 May;177(9):2408-15. doi: 10.1128/jb.177.9.2408-2415.1995.
1995 May degradation 2 1 GH13_31, GH13
PUL0277 gene deletion mutant and growth assay, qRT-PCR fructooligosaccharide Streptococcus pneumoniae 23264576
The ABC transporter encoded at the pneumococcal fructooligosaccharide utilization locus determines the ability to utilize long- and short-chain fructooligosaccharides. J Bacteriol. 2013 Mar;195(5):1031-41. doi: 10.1128/JB.01560-12. Epub 2012 Dec 21.
2013 Mar degradation 5 1 GH32
PUL0278 gene deletion mutant and growth assay, qRT-PCR fructooligosaccharide Streptococcus pneumoniae 23264576
The ABC transporter encoded at the pneumococcal fructooligosaccharide utilization locus determines the ability to utilize long- and short-chain fructooligosaccharides. J Bacteriol. 2013 Mar;195(5):1031-41. doi: 10.1128/JB.01560-12. Epub 2012 Dec 21.
2013 Mar degradation 6 1 GH32
PUL0279 gene deletion mutant and growth assay, qRT-PCR fructooligosaccharide Streptococcus pneumoniae 23264576
The ABC transporter encoded at the pneumococcal fructooligosaccharide utilization locus determines the ability to utilize long- and short-chain fructooligosaccharides. J Bacteriol. 2013 Mar;195(5):1031-41. doi: 10.1128/JB.01560-12. Epub 2012 Dec 21.
2013 Mar degradation 6 1 GH32
PUL0293 RT-qPCR, gene deletion mutant and growth assay mannan Bacillus sp. N16-5 29360976
Transcriptional regulation of the mannan utilization genes in the alkaliphilic Bacillus sp. N16-5. FEMS Microbiol Lett. 2018 Feb 1;365(4). pii: 4816728. doi: 10.1093/femsle/fnx280.
2018 Feb 1 degradation 12 7 GH130, GH130, GH27, CE7
PUL0302 RT-qPCR, isothermal titration calorimetry, enzyme activity assay, gene deletion mutant and growth assay arabinan Bacteroides thetaiotaomicron 29255254
Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol. 2018 Feb;3(2):210-219. doi: 10.1038/s41564-017-0079-1. Epub 2017 Dec 18.
2018 Feb degradation 22 6 GH51, GH146, GH43_4, GH43, GH43_4, GH51, GH43_29
PUL0304 RT-qPCR, isothermal titration calorimetry, enzyme activity assay, gene deletion mutant and growth assay galactan Bacteroides thetaiotaomicron 29255254
Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol. 2018 Feb;3(2):210-219. doi: 10.1038/s41564-017-0079-1. Epub 2017 Dec 18.
2018 Feb degradation 7 2 GH2, GH53
PUL0305 RT-qPCR, isothermal titration calorimetry, enzyme activity assay, gene deletion mutant and growth assay homogalacturonan Bacteroides thetaiotaomicron 29255254
Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol. 2018 Feb;3(2):210-219. doi: 10.1038/s41564-017-0079-1. Epub 2017 Dec 18.
2018 Feb degradation 17 7 GH105, CE8, CE4, CE8, CE12, PL1_2, PL1, CE8, PL1_2, PL1, CE8, PL1_2, GH28
PUL0306 RT-qPCR, isothermal titration calorimetry, enzyme activity assay, gene deletion mutant and growth assay galactan Bacteroides ovatus 29255254
Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol. 2018 Feb;3(2):210-219. doi: 10.1038/s41564-017-0079-1. Epub 2017 Dec 18.
2018 Feb degradation 7 3 GH2, GH53, GH147
PUL0313 microarray, Northern Blot, RT-qPCR, electrophoretic mobility shift assay, clone and expression, gene deletion mutant and growth assay alginate Zobellia galactanivorans 28983288, 32585009
Gene Expression Analysis of Zobellia galactanivorans during the Degradation of Algal Polysaccharides Reveals both Substrate-Specific and Shared Transcriptome-Wide Responses. Regulation of alginate catabolism involves a GntR family repressor in the marine flavobacterium Zobellia galactanivorans DsijT. Front Microbiol. 2017 Sep 21;8:1808. doi: 10.3389/fmicb.2017.01808. eCollection 2017. Nucleic Acids Res. 2020 Aug 20;48(14):7786-7800. doi: 10.1093/nar/gkaa533.
2017,2020 Aug 20 degradation 3 3 PL6, PL6_1, PL7_5, PL7, PL6, PL6_1
PUL0326 gene deletion mutant and growth assay, enzyme activity assay, thin layer chromatography beta-glucan Bacteroides ovatus 28461332
A Bacteroidetes locus dedicated to fungal 1,6-beta-glucan degradation: Unique substrate conformation drives specificity of the key endo-1,6-beta-glucanase. J Biol Chem. 2017 Jun 23;292(25):10639-10650. doi: 10.1074/jbc.M117.787606. Epub 2017 May 1.
2017 Jun 23 degradation 13 2 GH73
PUL0327 microarray, gas chromatography and mass spectrometry, gene deletion mutant and growth assay, complementation study maltodextrin Enterococcus faecalis 28455338, 32680872
Enzymes Required for Maltodextrin Catabolism in Enterococcus faecalis Exhibit Novel Activities. Enterococcus faecalis Maltodextrin Gene Regulation by Combined Action of Maltose Gene Regulator MalR and Pleiotropic Regulator CcpA. Appl Environ Microbiol. 2017 Jun 16;83(13). pii: AEM.00038-17. doi: 10.1128/AEM.00038-17. Print 2017 Jul 1. Appl Environ Microbiol. 2020 Sep 1;86(18). pii: AEM.01147-20. doi: 10.1128/AEM.01147-20. Print 2020 Sep 1.
2017 Jul 1,2020 Sep 1 degradation 6 3 GH13, CBM34, GH13_20
PUL0342 enzyme activity assay, gene deletion mutant and growth assay xylan Prevotella ruminicola 19304844
Biochemical analysis of a beta-D-xylosidase and a bifunctional xylanase-ferulic acid esterase from a xylanolytic gene cluster in Prevotella ruminicola 23. J Bacteriol. 2009 May;191(10):3328-38. doi: 10.1128/JB.01628-08. Epub 2009 Mar 20.
2009 May degradation 5 3 GH10, CE1, CBM22, GH95, GH3
PUL0343 gene deletion mutant and growth assay, enzyme activity assay, Western Blot, isothermal titration calorimetry carboxymethylcellulose, xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 39 1 GH5, GH5_2
PUL0344 gene deletion mutant and growth assay, protein structure characterization chitin Flavobacterium johnsoniae 27933102, 32792608
A polysaccharide utilization locus from Flavobacterium johnsoniae enables conversion of recalcitrant chitin. Structural insights of the enzymes from the chitin utilization locus of Flavobacterium johnsoniae. Biotechnol Biofuels. 2016 Nov 28;9:260. doi: 10.1186/s13068-016-0674-z. eCollection 2016. Sci Rep. 2020 Aug 13;10(1):13775. doi: 10.1038/s41598-020-70749-w.
2016,2020 Aug 13 degradation 11 3 GH18, GH20, GH18
PUL0346 gene deletion mutant and growth assay xylooligosaccharide uncultured bacterium 27573446
Functional characterization of a gene locus from an uncultured gut Bacteroides conferring xylo-oligosaccharides utilization to Escherichia coli. Mol Microbiol. 2016 Nov;102(4):579-592. doi: 10.1111/mmi.13480. Epub 2016 Sep 14.
2016 Nov degradation 15 5 GH43, GH43_29, GH43, GH43_12, GH43, GH43_1, GH10, GH16
PUL0347 gene deletion mutant and growth assay, RT-PCR sorbitol Bifidobacterium breve 24581150
Comparative genomics of the Bifidobacterium breve taxon. BMC Genomics. 2014 Mar 1;15:170. doi: 10.1186/1471-2164-15-170.
2014 Mar 1 degradation 13 1 CBM48, GH13_11
PUL0360 gene deletion mutant and growth assay lactose, host glycan Bacteroides thetaiotaomicron 16968696
Functional genomic and metabolic studies of the adaptations of a prominent adult human gut symbiont, Bacteroides thetaiotaomicron, to the suckling period. J Biol Chem. 2006 Nov 24;281(47):36269-79. doi: 10.1074/jbc.M606509200. Epub 2006 Sep 12.
2006 Nov 24 degradation 6 2 GH2, GH53
PUL0381 microarray, gene deletion mutant and growth assay chitin Vibrio cholerae 14983042
The Vibrio cholerae chitin utilization program. Proc Natl Acad Sci U S A. 2004 Feb 24;101(8):2524-9. doi: 10.1073/pnas.0308707101.
2004 Feb 24 degradation 6 1 CBM12, CE4, GH4
PUL0387 gene deletion mutant and growth assay, qRT-PCR, GlcNAc phosphorylation assays N-acetylglucosamine Xanthomonas campestris pv. campestris 20081036
Identification and regulation of the N-acetylglucosamine utilization pathway of the plant pathogenic bacterium Xanthomonas campestris pv. campestris. J Bacteriol. 2010 Mar;192(6):1487-97. doi: 10.1128/JB.01418-09. Epub 2010 Jan 15.
2010 Mar degradation 7 1 CE9
PUL0396 gene deletion mutant and growth assay N-acetylglucosamine Cupriavidus necator 21478317
Effects of homologous phosphoenolpyruvate-carbohydrate phosphotransferase system proteins on carbohydrate uptake and poly(3-Hydroxybutyrate) accumulation in Ralstonia eutropha H16. Appl Environ Microbiol. 2011 Jun;77(11):3582-90. doi: 10.1128/AEM.00218-11. Epub 2011 Apr 8.
2011 Jun degradation 7 1 CE9
PUL0397 gene deletion mutant and growth assay N-acetylglucosamine Capnocytophaga canimorsus 21762219
The genome and surface proteome of Capnocytophaga canimorsus reveal a key role of glycan foraging systems in host glycoproteins deglycosylation. Mol Microbiol. 2011 Aug;81(4):1050-60. doi: 10.1111/j.1365-2958.2011.07750.x. Epub 2011 Jul 18.
2011 Aug degradation 5 1 GH18
PUL0398 gene deletion mutant and growth assay N-acetylglucosamine Capnocytophaga canimorsus 21762219
The genome and surface proteome of Capnocytophaga canimorsus reveal a key role of glycan foraging systems in host glycoproteins deglycosylation. Mol Microbiol. 2011 Aug;81(4):1050-60. doi: 10.1111/j.1365-2958.2011.07750.x. Epub 2011 Jul 18.
2011 Aug degradation 9 1 GH2
PUL0475 clone and expression, gene deletion mutant and growth assay cellobiose, cellotriose Streptomyces reticuli 10347054
Characterization of the binding protein-dependent cellobiose and cellotriose transport system of the cellulose degrader Streptomyces reticuli. Appl Environ Microbiol. 1999 Jun;65(6):2636-43. doi: 10.1128/AEM.65.6.2636-2643.1999.
1999 Jun degradation 7 1 GH18, CBM2
PUL0519 gene deletion mutant and growth assay maltose Streptococcus pneumoniae 8244973
Characterization of the Streptococcus pneumoniae maltosaccharide regulator MalR, a member of the LacI-GalR family of repressors displaying distinctive genetic features. J Biol Chem. 1993 Dec 5;268(34):25402-8.
1993 Dec 5 degradation 7 2 GT35, GH77
PUL0555 gene deletion mutant and growth assay, qRT-PCR, microarray, enzyme activity assay N-glycan Bacteroides fragilis 25139987
Efficient utilization of complex N-linked glycans is a selective advantage for Bacteroides fragilis in extraintestinal infections. Proc Natl Acad Sci U S A. 2014 Sep 2;111(35):12901-6. doi: 10.1073/pnas.1407344111. Epub 2014 Aug 19.
2014 Sep 2 degradation 9 5 GH154, GH2, GH20, GH88, PL0, PL33_1
PUL0556 gene deletion mutant and growth assay, qRT-PCR, microarray, enzyme activity assay N-glycan Bacteroides fragilis 25139987
Efficient utilization of complex N-linked glycans is a selective advantage for Bacteroides fragilis in extraintestinal infections. Proc Natl Acad Sci U S A. 2014 Sep 2;111(35):12901-6. doi: 10.1073/pnas.1407344111. Epub 2014 Aug 19.
2014 Sep 2 degradation 7 1 GH18
PUL0557 gene deletion mutant and growth assay alpha-mannan Bacteroides thetaiotaomicron 25567280
Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism. Nature. 2015 Jan 8;517(7533):165-169. doi: 10.1038/nature13995.
2015 Jan 8 degradation 13 6 GH97, GH67, GH76, GH92, GH76, GH125
PUL0558 gene deletion mutant and growth assay, growth assay, enzyme activity assay rhamnogalacturonan Bacteroides thetaiotaomicron 28329766
Complex pectin metabolism by gut bacteria reveals novel catalytic functions. Nature. 2017 Apr 6;544(7648):65-70. doi: 10.1038/nature21725. Epub 2017 Mar 22.
2017 Apr 6 degradation 50 20 GH2, GH139, GH106, GH2, GH2, GH2, CBM57, GH137, GH138, GH78, GH141, GH127, GH95, GH105, GH140, CBM67, GH33, GH78, GH28, CBM67, GH78, GH142, GH143, GH43_18, GH43, CE8, PL1_2, PL1
PUL0559 gene deletion mutant and growth assay, growth assay, enzyme activity assay rhamnogalacturonan Bacteroides thetaiotaomicron 28329766
Complex pectin metabolism by gut bacteria reveals novel catalytic functions. Nature. 2017 Apr 6;544(7648):65-70. doi: 10.1038/nature21725. Epub 2017 Mar 22.
2017 Apr 6 degradation 12 5 CBM32, GH43_34, GH43_10, GH97, GH29
PUL0575 microarray, growth assay, gene deletion mutant and growth assay raffinose Enterococcus faecium 20946531
A genetic element present on megaplasmids allows Enterococcus faecium to use raffinose as carbon source. Environ Microbiol. 2011 Feb;13(2):518-28. doi: 10.1111/j.1462-2920.2010.02355.x. Epub 2010 Oct 15.
2011 Feb degradation 11 2 GH13, GH13_18, GH13_31, GH13, GH36, GH4
PUL0582 NMR, microarray, enzyme activity assay, gene deletion mutant and growth assay lactose Lactococcus lactis 22660716
A specific mutation in the promoter region of the silent cel cluster accounts for the appearance of lactose-utilizing Lactococcus lactis MG1363. Appl Environ Microbiol. 2012 Aug;78(16):5612-21. doi: 10.1128/AEM.00455-12. Epub 2012 Jun 1.
2012 Aug degradation 5 1 GH1
PUL0583 enzyme activity assay, gene deletion mutant and growth assay cellobiose Geobacillus stearothermophilus 8407820
Cloning and sequencing of a cellobiose phosphotransferase system operon from Bacillus stearothermophilus XL-65-6 and functional expression in Escherichia coli. J Bacteriol. 1993 Oct;175(20):6441-50. doi: 10.1128/jb.175.20.6441-6450.1993.
1993 Oct degradation 5 1 CE0
PUL0585 microarray, gene deletion mutant and growth assay, beta-galactosidase assays cellobiose Streptococcus pneumoniae 21778207
CelR-mediated activation of the cellobiose-utilization gene cluster in Streptococcus pneumoniae. Microbiology (Reading). 2011 Oct;157(Pt 10):2854-2861. doi: 10.1099/mic.0.051359-0. Epub 2011 Jul 21.
2011 Oct degradation 7 1 GH1
PUL0590 qRT-PCR, gene deletion mutant and growth assay, microarray maltose, maltodextrin Enterococcus faecium 23951303
A LacI-family regulator activates maltodextrin metabolism of Enterococcus faecium. PLoS One. 2013 Aug 7;8(8):e72285. doi: 10.1371/journal.pone.0072285. eCollection 2013.
2013 degradation 5 1 CBM34, GH13_20
PUL0605 RT-PCR, gene deletion mutant and growth assay glycogen Escherichia coli 33101261, 21029047
Glycogen Metabolism Impairment via Single Gene Mutation in the glgBXCAP Operon Alters the Survival Rate of Escherichia coli Under Various Environmental Stresses. Escherichia coli glycogen genes are organized in a single glgBXCAP transcriptional unit possessing an alternative suboperonic promoter within glgC that directs glgAP expression. Front Microbiol. 2020 Sep 25;11:588099. doi: 10.3389/fmicb.2020.588099. eCollection 2020. Biochem J. 2011 Jan 1;433(1):107-17. doi: 10.1042/BJ20101186.
2020,2011 Jan 1 biosynthesis 5 4 GT35, GT5, GH13, GH13_11, CBM48, GH77, GH13, GH13_9, CBM48