CAZyme Information: MGYG000000345_02361

Basic Information

• Species: UBA1829 sp002338895
• Lineage: Bacteria; Verrucomicrobiota; Lentisphaeria; Victivallales; UBA1829; UBA1829; UBA1829 sp002338895
• CAZyme ID: MGYG000000345_02361
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1364		154570.27	9.0496

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000345	3773289	MAG	Sweden	Europe

• Gene Location: Start: 36401; End: 40495 Strand: +

Full Sequence      

MKSCLPLFSLFLTVSLLAAEIPPWLPPANGNIPRLETVSHPAIPDGPAATPGAFCIERSL
NGIWKLSNLENSVSPFSDSPEEERRFSAVDLDDSAWNRIPVPLNWYEKFPGKRVKGKPYV
RGWYRTEFLLTGQEREGKRVVLKFDVAGYDAKVFLNGKYLGSHKGDFTPFELDATDAAKN
GKNVLAVRIISDLGTTFGLKQVSHPYGAQWGGYSIKGGLWQSVRLSLEPEVRIRKLLVTP
FIRENRIEVDYTIYNHTGKTLTAEMVAQITPAARGAHLQRSGRNTEKRNLLPGESKGRMS
ISLNDPRLWTPENPWLYYLNFRILVNDTPVSGAIRRFGFREFRTADGKFYLNGKQVYLFG
QNLSSVNYGGKRTVEEDRKRLEDDIRKIRSLGYNIVRTPHMPILPLALELCDEFGLMVYY
EWAWCFDNQLAPDFESNNLRELGEFVETGYNHPSVVMWVLGNEIRHRDRPDVVRLLDRQT
AEVRRLDRSRRPISTFSGAAGWLSYGRNKLDTDVLDFHNYTSLYGAWTGMPDVFRKIQTG
LEEIYGSDFPQALVAWENVGFSWGARPDPAFRRGDRKLYAAQMRKPTSWGMPNGVGFVGS
SPLFKALSPGFGEWAQTRFGHRIFEYYRRNPHYAGFSPWFNPIPASTLWTQRVYPSLCDR
NNLFPHNLFSGETTQWNLRIANDGSETFRNVSLELSLFDGEREQTFARLPIPELPAHGAW
AKPYSLTLPKLSGSGFRQLRLALKQGERRIGQNFYDLFLADRAELEGPLSAARAVRILDT
GAAGNVRELADILTRLRIPFEKISSIGRAPAGSVVIVPPEIREEQTLNLNGSEITEWIQR
KQGTLLVLEQRNPRSIYPGDRRLTADSVAYTDMVLPDHPIFRGMEYRNLESWNNPDHAYV
VDFSFMPYSEEALAVKGPRFSHSNVGCSLVELRSGGGRMIFSQLNAVRCAPVDPSASLYL
RNLLAYATGQEYEKDAPELDLEKNREYRISEKRAVAVDLSASANRSFRDDVSNDGKGGWT
DEGENDFRTMPLGNLRAAGIPFHILDPEKNHGKSCLVVRGTVRPHLPAAIRGIRIGRKFS
RIFFLHTSTWGGNNQAGSYRFHYADGSVENCPLIGGWNIGDWWNVFSLPSARIGLQRENP
KGQKVACYVTEWENPKPEVEITSMDFLSLSASREGEIDWNPENAAIPVLIAVTGEETNPN
RLKLTGKADFLTARGIREVLSKVSGEVKIRKEKEDVCLEVFFPSYQGSNVPGAWIGFRKE
GVRGPYESLTCEIRSRDGGALQLRLPGEKWKDFWTAYLNVPGDGKWHSYRLFFDRDFQSF
RGNGKNPIDFTRIRPELFLFSRPKFKLEGDVPAHTFEIRNVVLE

Enzyme Prediction     

No EC number prediction in MGYG000000345_02361.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	58	519	8.3e-82	0.48404255319148937

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	8.46e-50	57	498	12	434	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	1.39e-39	60	463	44	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK10150	PRK10150	4.57e-29	58	463	13	417	beta-D-glucuronidase; Provisional
PRK09525	lacZ	6.34e-19	58	463	53	462	beta-galactosidase.
pfam02836	Glyco_hydro_2_C	9.21e-14	342	463	1	135	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AVM46610.1	0.0	5	1363	9	1364
AVM44624.1	0.0	18	1364	19	1372
AVM45701.1	0.0	18	1364	24	1377
AVM45809.1	0.0	19	1364	22	1369
AVM43171.1	0.0	18	1364	20	1374

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6ED1_A	5.25e-37	81	464	38	422	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Phocaeicola dorei],6ED1_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Phocaeicola dorei],6ED1_C Chain C, Glycosyl hydrolase family 2, sugar binding domain protein [Phocaeicola dorei],6ED1_D Chain D, Glycosyl hydrolase family 2, sugar binding domain protein [Phocaeicola dorei]
5T98_A	7.23e-33	88	571	46	538	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]
6D8G_A	3.16e-31	58	526	36	501	D341AD367A calcium binding mutant of Bacteroides uniformis beta-glucuronidase 2 [Bacteroides uniformis str. 3978 T3 ii],6D8G_B D341A D367A calcium binding mutant of Bacteroides uniformis beta-glucuronidase 2 [Bacteroides uniformis str. 3978 T3 ii]
5UJ6_A	1.62e-30	58	526	28	493	CrystalStructure of Bacteroides Uniformis beta-glucuronidase [Bacteroides uniformis str. 3978 T3 ii],5UJ6_B Crystal Structure of Bacteroides Uniformis beta-glucuronidase [Bacteroides uniformis str. 3978 T3 ii],6NZG_A Bacteroides uniformis beta-glucuronidase 2 covalently bound to cyclophellitol-6-carboxylate aziridine [Bacteroides uniformis],6NZG_B Bacteroides uniformis beta-glucuronidase 2 covalently bound to cyclophellitol-6-carboxylate aziridine [Bacteroides uniformis]
6D50_A	1.65e-30	58	526	36	501	Bacteroidesuniforms beta-glucuronidase 2 bound to D-glucaro-1,5-lactone [Bacteroides uniformis str. 3978 T3 ii],6D50_B Bacteroides uniforms beta-glucuronidase 2 bound to D-glucaro-1,5-lactone [Bacteroides uniformis str. 3978 T3 ii]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KPJ7	5.66e-37	91	464	74	431	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
Q6LL68	8.74e-30	24	466	12	459	Beta-galactosidase OS=Photobacterium profundum (strain SS9) OX=298386 GN=lacZ PE=3 SV=1
Q56307	1.61e-29	59	463	41	441	Beta-galactosidase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=lacZ PE=1 SV=2
P77989	2.35e-29	80	458	16	382	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
Q8D4H3	5.29e-28	24	466	13	461	Beta-galactosidase OS=Vibrio vulnificus (strain CMCP6) OX=216895 GN=lacZ PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000308	0.999047	0.000155	0.000162	0.000143	0.000140

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000345_02361.