CAZyme Information: MGYG000000380_00501

Basic Information

• Lineage: Bacteria; Firmicutes; Bacilli; Erysipelotrichales; Erysipelotrichaceae; Merdibacter
• CAZyme ID: MGYG000000380_00501
• CAZy Family: GH29
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1374		148199.06	3.8757

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000380	1622044	MAG	Sweden	Europe

• Gene Location: Start: 2042; End: 6166 Strand: -

Full Sequence      

MKLTKIALSAAMAMSVFAGIPLTTTVFAAENYVYDGDLSEKGTAAPDAWGATPTPEQYRY
QKEELAGFVHFGPNTFNNIEWGENYGDRTPDEIFMLEEDFDAETLVKAFHDAGFKKIIVT
AKHHDGFCIWNSAYTEYDVASTSYKGGQGDILAEISAACTKYDVDMGLYLSPWDIHDESY
GYYDAEGNPTDAEQDVLDYNDYYVNQLNEILGDDKYGNNGHFTEIWMDGAKGSGANAQDY
DFQRWYDTIQAQEGVQAGYDSECMIFQCGANTTVRWIGNENGYAAKNTWSKSIVDVEADT
CDDNRQGSYAIGYEYGNKWTVPEADTRITSGWFWGPNKNTPKSITDLADMYFNSIGHNAP
LLLNVPPNTEGTVDQAILDRLAEFGANIEETFANNLAADAVIGASTVRGNDAAFAPSNVL
DGNDATYWATDDGTSEGSLIIDLGETKTFDVVSVEEAIQLGQSINEYKVEYRNGENSEWT
LLDEGETIGAKRLIRTAAVRADQLRITVDTNKNDAVPMISEVGVYKASEDFELTGTAPIG
MDVIDIEDTDVSDGAGFAFTNGTWIAESGTNFINGTNRYANAGASLTLTFHGSKVYLLGT
KDPNHGTASISIDGGDPVTIDTEADARALGQMLFASEDLADGDHTLTLTVGDSGAIGLEA
AYVINNGGVGMIGLEQDSYTMNEDSSMEVKIIRVGGTTGTIHALLQPNPGTAIQDDFDTE
QITEIVMAEGQSEATATVTTRRNTNVTGDRQFSIELVDPSDGLIIGFNDRAQITIKDTET
VSLEELDALLEEARERKRDWYVSGWENFAQALTFAETIADMSDPDGNDVTKAIEDLRSTM
DALVARERYTESDRFRFPWREGSSAVLEAEFATELINDESNDNGWPMEVSEAEWASNGKF
LNSINAGDVAKFAYTAEKAGTYHVTAYYRSGSNTNALAWSEENGKIESGTAAAGASSAET
THTAEFDLIVTEAGDGTLVFTGPSGRSPQLDRLEIVPVDVELAEYTITATAGVGGTISDA
GSTTLTEGSNKTYAITANEGYAIADVLVNGTSVGAVSTYTFEDLRADATIEARFVFDHYT
ADDPFRFPSEGNSATLEAEHVSQILNDESNDNGWPCVVTEGDWASNGKFVDALNQGDVIK
YHYRADEVGTYHVVVTYRSGSNANGLAWSEESGKIESGTVAAGADDAGVTHTAEFDLLVT
QAGDGVLVFTGPDGKSPQLDKFEITLTREPEPTPVDKTQLNETIQDAQALDAKDHTDESW
AALQEALRQAERVAADANADQAAVDAAEQALSDAIAALEDAPKEELPGTGDEDPQDPNQP
AADDTDETPDPEQPTDSDEEERADTATADMSGALIALALAAGAASVVTYRRKRS

Enzyme Prediction     

No EC number prediction in MGYG000000380_00501.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH29	53	387	5.9e-61	0.861271676300578

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3669	AfuC	6.29e-62	50	525	8	430	Alpha-L-fucosidase [Carbohydrate transport and metabolism].
smart00812	Alpha_L_fucos	6.77e-30	53	385	18	327	Alpha-L-fucosidase. O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site. Because the fold of proteins is better conserved than their sequences, some of the families can be grouped in 'clans'. Family 29 encompasses alpha-L-fucosidases, which is a lysosomal enzyme responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. Deficiency of alpha-L-fucosidase results in the lysosomal storage disease fucosidosis.
pfam01120	Alpha_L_fucos	3.39e-29	98	386	82	326	Alpha-L-fucosidase.
pfam00754	F5_F8_type_C	1.37e-08	402	522	2	127	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
cd00057	FA58C	1.02e-04	394	480	6	90	Substituted updates: Jan 31, 2002

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
PLT74914.1	0.0	23	1250	35	1264
QHB23055.1	0.0	23	1279	35	1299
QRT29448.1	0.0	23	1279	35	1299
QEI30547.1	0.0	23	1279	35	1299
QPK81604.1	0.0	15	994	15	1001

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6TR3_A	2.56e-238	21	575	2	552	Ruminococcusgnavus GH29 fucosidase E1_10125 in complex with fucose [[Ruminococcus] gnavus E1]
6TR4_A	4.02e-237	21	575	2	552	Ruminococcusgnavus GH29 fucosidase E1_10125 D221A mutant in complex with fucose [[Ruminococcus] gnavus E1],6TR4_B Ruminococcus gnavus GH29 fucosidase E1_10125 D221A mutant in complex with fucose [[Ruminococcus] gnavus E1]
6ORG_A	6.08e-100	50	526	8	449	Crystalstructure of SpGH29 [Streptococcus pneumoniae TIGR4],6ORG_B Crystal structure of SpGH29 [Streptococcus pneumoniae TIGR4]
6OR4_A	7.68e-99	50	526	8	449	Crystalstructure of SpGH29 [Streptococcus pneumoniae TIGR4],6OR4_B Crystal structure of SpGH29 [Streptococcus pneumoniae TIGR4],6ORH_A Crystal structure of SpGH29 [Streptococcus pneumoniae TIGR4],6ORH_B Crystal structure of SpGH29 [Streptococcus pneumoniae TIGR4]
6ORF_A	7.92e-99	50	526	8	449	Crystalstructure of SpGH29 [Streptococcus pneumoniae TIGR4],6ORF_B Crystal structure of SpGH29 [Streptococcus pneumoniae TIGR4]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8GW72	5.01e-91	53	525	38	477	Alpha-L-fucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=FUC1 PE=1 SV=2
Q7XUR3	1.29e-90	53	528	40	479	Putative alpha-L-fucosidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0560400 PE=3 SV=2
Q9BTY2	1.21e-07	49	386	61	358	Plasma alpha-L-fucosidase OS=Homo sapiens OX=9606 GN=FUCA2 PE=1 SV=2
Q5RFI5	2.75e-07	49	386	59	356	Plasma alpha-L-fucosidase OS=Pongo abelii OX=9601 GN=FUCA2 PE=2 SV=1
P10901	4.34e-06	100	385	105	350	Alpha-L-fucosidase OS=Dictyostelium discoideum OX=44689 GN=alfA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000268	0.998972	0.000186	0.000204	0.000175	0.000156

TMHMM  Annotations     

start	end
7	29
1352	1369