CAZyme Information: MGYG000000630_00384

Basic Information

• Lineage: Bacteria; Firmicutes_C; Negativicutes; Veillonellales; Dialisteraceae; Dialister
• CAZyme ID: MGYG000000630_00384
• CAZy Family: GT9
• CAZyme Description: ADP-heptose--LPS heptosyltransferase 2

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
359	MGYG000000630_8|CGC1	40233.32	7.1135

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000630	2325548	MAG	Madagascar	Africa

• Gene Location: Start: 28830; End: 29909 Strand: +

Full Sequence      

MEDRYYHVDLKNKTIVLIYMLYFGDMVSISPFLEVLRREAEGSKILLVMDARFQEAVKYN
PNIDEIIPFDRHGREKGMGATWKLGKKIGAFNPDILLTLHGTTRSTLLALAMHPKHWGGE
EGTRIDSFFMDWPMTIETYNSHAVDKYLKVLERLGVKDLHHSGMRTFTSPEWEKKADDFY
RARGISPKDRLVGLSVGSSTAEKNWPAEKYGKTADHFAEKGMIPVFFGVPSELPLIEKAV
SVMKSKRYIIAAGKLSMGEFMAAAGRCSLAFTNDSGPMYVFDSRGVPTIAMFGPSNAKFH
HPLGPCSKAISSWDMPMGPEHVNKTIASGQYTPISEIPLEKVIETGEWALAESESVEKE

Enzyme Prediction     

No EC number prediction in MGYG000000630_00384.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT9	82	310	8.2e-38	0.8888888888888888

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd03789	GT9_LPS_heptosyltransferase	7.97e-46	14	344	1	273	lipopolysaccharide heptosyltransferase and similar proteins. Lipopolysaccharide heptosyltransferase (2.4.99.B6) is involved in the biosynthesis of lipooligosaccharide (LOS). Lipopolysaccharide (LPS) is a major component of the outer membrane of gram-negative bacteria. LPS heptosyltransferase transfers heptose molecules from ADP-heptose to 3-deoxy-D-manno-octulosonic acid (KDO), a part of the inner core component of LPS. This family also contains lipopolysaccharide 1,2-N-acetylglucosaminetransferase EC 2.4.1.56 and belongs to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.
COG0859	RfaF	1.33e-37	13	344	2	324	ADP-heptose:LPS heptosyltransferase [Cell wall/membrane/envelope biogenesis].
pfam01075	Glyco_transf_9	2.02e-25	82	316	2	235	Glycosyltransferase family 9 (heptosyltransferase). Members of this family belong to glycosyltransferase family 9. Lipopolysaccharide is a major component of the outer leaflet of the outer membrane in Gram-negative bacteria. It is composed of three domains; lipid A, Core oligosaccharide and the O-antigen. All of these enzymes transfer heptose to the lipopolysaccharide core.
PRK10422	PRK10422	2.83e-10	9	357	2	350	lipopolysaccharide core biosynthesis protein; Provisional
PRK10916	PRK10916	7.79e-05	203	310	195	305	ADP-heptose--LPS heptosyltransferase RfaF.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BBK25206.1	9.17e-189	1	356	1	357
AXL20515.1	1.63e-91	8	347	2	339
AXB80808.1	2.20e-84	8	347	2	339
ALG42175.1	4.02e-84	8	351	2	343
AVO27634.1	4.02e-84	8	351	2	343

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3TOV_A	2.65e-71	9	353	5	348	Thecrystal structure of the glycosyl transferase family 9 from Veillonella parvula DSM 2008 [Veillonella parvula DSM 2008],3TOV_B The crystal structure of the glycosyl transferase family 9 from Veillonella parvula DSM 2008 [Veillonella parvula DSM 2008]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000056	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000630_00384.