CAZyme Information: MGYG000001333.1_02021

Basic Information

• Species: Lentilactobacillus hilgardii
• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lentilactobacillus; Lentilactobacillus hilgardii
• CAZyme ID: MGYG000001333.1_02021
• CAZy Family: GH2
• CAZyme Description: Beta-glucuronidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
603	MGYG000001333.1_106|CGC4	69463.63	4.8812

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001333.1	3077266	Isolate	not provided	not provided

• Gene Location: Start: 444056; End: 445867 Strand: +

Full Sequence      

MEKGLLYPVINNYRSDEKMDGLWNFKFDPAYEGESKGWQNGFNDGVKMPVPASFNDFFTD
KDAREYTGDFWYSKTFFVPEYFEGRDVQIRFGAATHRAVVYVNGQEIRSHEGGFLPFTAE
VTNAVKFGEENIVSVKINNELHRDALPAGDTATLKNGKKMAKPFFDFYNYSGLNRSVHLL
TVPKNGIYDYSTSYEVGDAEATVHYSIQADSGDDVKVSLINEDDQIVATGEGNTGDLKVD
HPNLWNVRDAYLYTIKFELMNDGKIVDEYADQIGLRTIEIKGHEILVNHKSVYLRGFGRH
EDSIYAGRGFDLNVERRDMNLMKWIGANSFRSSHYPYDEQVYKFADREGILVIDEVPAVG
FKMAAASFLGGLNQSFFDGDWTHELYKKHLDQIIDMIKRDKNHPSVLAWSLFNEPDTSNE
SAVPYFEKLFAATADLDPEKRPRTFTLNEDDTYETSKCLKFPDFYLLNRYPGWYHKWGYE
ISDGEAGLRAELDEWQKSDIDKPIVFSEYGADTEPGLHKLPSIMWSEEYQIELLEMFDRV
FDSYDFIRGEQPWNLADFQTVEGNMRVNGNKKGIFTRDRQPKAAAFYLKKRWTKLPLNYK
AGK

Enzyme Prediction     

No EC number prediction in MGYG000001333.1_02021.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	14	593	2.2e-98	0.6117021276595744

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10150	PRK10150	0.0	5	603	1	602	beta-D-glucuronidase; Provisional
COG3250	LacZ	9.31e-109	13	601	9	605	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam02836	Glyco_hydro_2_C	8.43e-73	278	594	1	299	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
PRK10340	ebgA	2.39e-35	72	438	113	468	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	5.91e-27	166	414	202	462	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QHB50849.1	0.0	1	603	1	603
AFR99354.1	0.0	1	603	1	603
QUX06513.1	0.0	1	603	1	603
AEB72488.1	0.0	1	603	1	603
APR06983.1	0.0	1	603	1	603

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6ECA_A	0.0	1	601	25	626	Lactobacillusrhamnosus Beta-glucuronidase [Lacticaseibacillus rhamnosus],6ECA_B Lactobacillus rhamnosus Beta-glucuronidase [Lacticaseibacillus rhamnosus]
7KGY_A	5.21e-225	3	600	4	605	ChainA, Beta-glucuronidase [Faecalibacterium prausnitzii],7KGY_B Chain B, Beta-glucuronidase [Faecalibacterium prausnitzii],7KGY_C Chain C, Beta-glucuronidase [Faecalibacterium prausnitzii],7KGY_D Chain D, Beta-glucuronidase [Faecalibacterium prausnitzii]
6ED2_A	1.88e-223	1	603	24	630	ChainA, Glycosyl hydrolase family 2, TIM barrel domain protein [Faecalibacterium duncaniae]
6JZ1_A	4.38e-220	1	594	2	598	Apostructure of b-glucuronidase from Ruminococcus gnavus at 1.7 Angstrom resolution [[Ruminococcus] gnavus],6JZ1_B Apo structure of b-glucuronidase from Ruminococcus gnavus at 1.7 Angstrom resolution [[Ruminococcus] gnavus],6JZ5_A b-glucuronidase from Ruminococcus gnavus in complex with D-glucuronic acid [[Ruminococcus] gnavus],6JZ5_B b-glucuronidase from Ruminococcus gnavus in complex with D-glucuronic acid [[Ruminococcus] gnavus],6JZ6_A b-glucuronidase from Ruminococcus gnavus in complex with C6-substituted uronic isofagomine [[Ruminococcus] gnavus],6JZ6_B b-glucuronidase from Ruminococcus gnavus in complex with C6-substituted uronic isofagomine [[Ruminococcus] gnavus],6JZ7_A b-glucuronidase from Ruminococcus gnavus in complex with N1-substituted uronic isofagomine [[Ruminococcus] gnavus],6JZ7_B b-glucuronidase from Ruminococcus gnavus in complex with N1-substituted uronic isofagomine [[Ruminococcus] gnavus],6JZ8_A b-glucuronidase from Ruminococcus gnavus in complex with D-glucaro 1,5-lactone [[Ruminococcus] gnavus],6JZ8_B b-glucuronidase from Ruminococcus gnavus in complex with D-glucaro 1,5-lactone [[Ruminococcus] gnavus]
6EC6_A	4.82e-220	1	594	25	621	Ruminococcusgnavus Beta-glucuronidase [[Ruminococcus] gnavus],6EC6_B Ruminococcus gnavus Beta-glucuronidase [[Ruminococcus] gnavus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P05804	5.65e-160	5	593	1	589	Beta-glucuronidase OS=Escherichia coli (strain K12) OX=83333 GN=uidA PE=1 SV=2
P06760	1.27e-131	1	595	23	625	Beta-glucuronidase OS=Rattus norvegicus OX=10116 GN=Gusb PE=1 SV=1
O97524	2.76e-131	4	595	26	628	Beta-glucuronidase OS=Felis catus OX=9685 GN=GUSB PE=1 SV=1
O18835	5.50e-131	1	595	23	628	Beta-glucuronidase OS=Canis lupus familiaris OX=9615 GN=GUSB PE=1 SV=1
P08236	7.75e-131	1	595	23	629	Beta-glucuronidase OS=Homo sapiens OX=9606 GN=GUSB PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000064	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001333.1_02021.