CAZyme Information: MGYG000002131_00427

Basic Information

• Species: CAG-882 sp900545455
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; CAG-882; CAG-882 sp900545455
• CAZyme ID: MGYG000002131_00427
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1160		131419.43	4.6934

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002131	2861336	MAG	United States	North America

• Gene Location: Start: 9348; End: 12830 Strand: -

Full Sequence      

MNKTVLWDDGWSFKKTSLGVTLNEALTEKFDAVDIPHDWMIYDTNNLYETSIGWYKKTFK
VSGDEIKKRLIVRFGAVYMDSTVYVNGREVCEWKYGYSTFDADITEYVHEGDNEIVVKVV
YQCPNTRWYSGAGIIRDVYFMTLEPFHIVPDGTYIRVRKNDDGTWKVKCAIEIENLWQDV
KVGEVQAGLTLIHGGSSVFTFGHIAGDEGDIFARGEKKIVELELTVDKPLIWDIDEPNLY
DCSVSLNYKDELLHEENQRIGFCTKEYSPEEGFLLNGRKVRINGVCEHHDNGCLGAAFNV
NAFRRKLNKLRKMGVNAIRTSHNMPAKEVLELADEMGFLVDCEAFDMWELPKTEFDYARF
FVDWADRDVASWIRRDRNHVSVIMWSIGNEIYDTHRDEHGQEITRRLLGYVRKHDPECNA
PVTIGSNYMPWENAQKCADIVKFAGYNYAEKYYEQHHKEHPDWIIYGSETASTLQSRGIY
KFPYEQRILCDDDEQCSSLGNCSTNWGAKNTEYCIIMDRDAHFSMGQFIWTGFDYIGEPT
PYQTKNSYFGQLDTAGFEKDTYYIYQAEWTDYKKAPMVHVFPYWDFSEGEVIDVRVASNA
PKVELFLNGRSLGAYDIDHKHGRELLAHYKVEYEPGVIEAVAYDENGEVIARDVKKSFSD
AAGLVVTPENTTVRADGRDLVYVDISAVDRDGNPVENANNRVHVKVTGAGRLVGMDNGDS
ADFDQYKTDSRRMFSGKLLAVIAAKTESGEIKLEASSDGMETVTVTMEALPCDRTEGVSA
GENICAVSETSTESDEIPVRNIRMKVKGSRVLSPDNMQVEIEAEILPHNVTYKELIWKVT
NDAGVDSNGAVLEDGKPSDVPYIVNKVIKAVGDGKHNVRCCTKNGREKISLISQISFIAE
GLGMAEFNPYEFVYGTLYTSAEVEPAVGQDRGIVPRGGTYIAFEKVNFGKNGSDEITLPV
YELNNKPFPIQIWKGIPGQDGAELIDTVIYDLPSIWATYQEKTYRLPKRFKGVCTLSFVA
DHDINIKGFSFKKYERAYDTNYAVDNDGIYGDTYTLESDAVTGIGNNVSLTYNDMSFTHG
LHRITICGRSVLEKNTIHVRFANEAGTENQIAEFVMPQKPESEDGYVEQSFALENVEGNC
SVNLVFLPGCNFDLKWFKFE

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	7	565	1.4e-101	0.589095744680851

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	4.19e-61	33	585	63	614	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam18565	Glyco_hydro2_C5	2.32e-40	664	766	1	103	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.
PRK10150	PRK10150	5.83e-35	54	441	68	464	beta-D-glucuronidase; Provisional
PRK10340	ebgA	2.75e-33	53	390	111	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	3.59e-25	31	390	83	462	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QLG36749.1	0.0	1	1160	1	1158
QOS77051.1	0.0	1	1160	1	1168
AGC67981.1	0.0	1	1160	1	1145
ANW98383.1	0.0	1	1160	1	1145
AGI39016.1	0.0	1	1160	1	1145

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7CWD_A	1.77e-165	3	767	2	799	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
4YPJ_A	4.85e-163	3	766	8	809	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
4CU6_A	2.47e-154	2	767	13	842	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU7_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU8_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4]
4CUC_A	6.78e-154	2	767	13	842	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA. [Streptococcus pneumoniae TIGR4]
5DMY_A	3.34e-134	2	776	36	891	Beta-galactosidase- construct 33-930 [Bifidobacterium bifidum],5DMY_B Beta-galactosidase - construct 33-930 [Bifidobacterium bifidum],5DMY_C Beta-galactosidase - construct 33-930 [Bifidobacterium bifidum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	1.20e-150	33	769	74	848	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	1.95e-88	7	750	51	801	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	1.05e-50	18	620	70	680	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P77989	1.91e-36	30	714	32	695	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
T2KN75	5.76e-35	2	779	30	805	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000062	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002131_00427.