CAZyme Information: MGYG000002256_01227

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; UBA3282
• CAZyme ID: MGYG000002256_01227
• CAZy Family: GH16
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2329	MGYG000002256_28|CGC2	254734.51	4.3541

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002256	2755304	MAG	Peru	South America

• Gene Location: Start: 17331; End: 24320 Strand: -

Full Sequence      

MRKKKPFFNRLITMTLCVSMVVTSAGIPQLHVEAAKETTDDTVQKPFGEVIGYDADQQAI
TVVWGNGDADGSLGYSYCVSITGQDTEESYAKEYDDLPCAAQAILYEGEGEGYEAGKTYL
VELQTKQDGNYSEAVSSAVKIPGGDTEPDAGEEEEGQEKGSDDEEIKLPVAPSGIVAGYS
DTQGYEGKISVTWGAAFGEGGTPTSWEVSIDGKLVGTETAVAAYYYDNIYEAGEHTVSVT
AVNEAGKSDPGETTFTLTEAQAGVGNDEEPKPEQESESAEIVISEQEAQAIAGNAVTLTY
VASSGFDFTDYTVTVNGQSVSTENIVIGENTIQMSADLFGAEGVYKIQFAKDGYTFTPVY
QSVFGTLNEKNTWHLVWNDEFNGTSIDRTKWDYQTGTGAEYGVGGWGNSEEEYYTDSPEN
SSVGNGTLTITAKKDNSRGTNYTSARLRTVTEEISGGKAGKGTPLKIGTYGRVEAKMKMP
TGNGVWPAFWMLPYDSRYGTWATSGELDILEARGRLPQDICGTIHYGDVWPNNVHNGKDY
TFQDSSIAEYHIYGVEWEPTEIRWMVDGEVYGTISNWYSVAGEEGNYAFPAPFDEEFYIL
LNLAVGGTFDSAVSSDQIQVDENGVCMDVDYVRWYQLDEEVYKNWNIKQSDADKDTSEEA
EALLKLADESGNFIKDKDFTQMNTTPYTKGGSWNIERGYWAPLLIPENGNGQATWSKVTR
DGENYLKVAVANAGSQTYSSQMLQYFPVVSGYSYEISYKAYTDAAATKADVALKIGGDDD
NSWAVYSGNYTDELTTTPKTYSHKFTMNADTDPTARFEFNLATSAGNVYLSDVCVKLTQI
NESDGEDDAKQPLSNGNHVYNGEFNAGSDGLLYWHWSGDDSKDVVSVSIGADKNRRADIK
IDNAPVSIWQKGINLLQKDSYSLSFTVDTQEAQDVIITLESVDGSQTYYTETKKASAGES
VISTTFTQPEGKTDTEAVLKITFAKSAALDAVKMIRTTNFNVDFDSMEIWPVYNGDFFNG
KDGWNIWSEGAGYITSDVNADGKLDAQVTVGADATFYCVGIQSQSMTLDKGIKYRVKFDY
EIPSEKNYKIELAGEQRDITLPAGAHTYVSEPFTGAGNSKFTLYLGPDQTATYRFLLDNV
EVYIDPDSITVPEGYAKPVSLAQKVQAKAQSDVVVNYTEDPVWEAAAKTYEVDGKQIDAD
LVEMDAANNTLTIAGSCFPESGSYGFAAKAEGYTKTKTIPLSILEASGNLLLNGSFASGT
DSWGFYYADWTAAGSFQVNEDGVAVIEHKYDGGEEWHFQLYQDGLEYMAGDYLVTFDAWS
DVERPIGVQLQNGNEVLAGTANKVLLSTQKQSYKLMMKGLAAGSGIKLDFPMGSMTYNGV
TAPNDGGSPYNIYLDNVVFRQVTQADIDSEPATIALAVTSQKIGQDVQVKYAEANNTWKA
AKKTVSINGTAAPADKVTESEDGMILDQSLFEKTGRYEISITAAGFEPTNSVFQRITAAD
GDLLLDGDMSDAALQTWTVYNEDPENLSKGSIADGKFQLDYKAGYYRSDWNVWVTWSSQL
KQDGILIEGGKKYILNFKASTDLKDGRDIVIEYGVTGKTAQKTVHIEEGSAYYQIELEHS
DNADDFYLVYLLGPTDAKLQVEGTVLHTMTLDDISLRESSSMDAFREAKAKELAETIGEK
ERYSKDNYTEESWKTFQEALQAAKEVDVETADLQTILDALDALNTAADNLTLLQGLRVDA
IEDQVYTGSKITPQIKVYDGSTLLGAKDYKVTYSKNVNVGTATVKVTGKGNYKGTDEVTF
QILPCPMENVTAEDVYAVISSKNKVTNPKVTVKYGRLTLKNNKDYSITWPEISYDGQNTP
VPGKYPVKISAKSANYTGDKQISLIVKEKDAISMSKVKVKVDPVSVAYNGEETPEPQVTV
TYGSGKKAQTLVKGEDYQVSFPDGWKKIGKVTVTIAGTENSRYYGTKTASFTVTGTKLTK
RQMTVTGIEASYEYTGSPIKVDDTLVVTDAAADHKMEQGTDYEVSYPKDKNCNVGKATVQ
ITGIGAYTGSLSFSFKITPADLSKAEVTGEDKAVYTNAGAKPIYTLTYQGLTLTEGKDYK
VACKNNKKIGKSAEVTISGKGNFKGTWKKTFEVTAPAEGAVIITAQDVVVPKKLNSLKTG
VVLKDVVSGKALKAGSDYDKKFTYYVKEQGQERAVTAEDLKADQEIWVRVTLKGNYVSAG
VVEDSFRLYTVKASTFKVDKIANQTYTGKEICPKVVVRSKSGVILEEHKDYEVRYEKNIQ
KGTAKVYVTGIGNGYGGTKQTSFKILPAQMKWYQSVATQMLEFFSNLVS

Enzyme Prediction     

No EC number prediction in MGYG000002256_01227.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	377	635	1.9e-75	0.9956521739130435
CBM4	672	821	2.6e-21	0.9841269841269841
CBM4	1249	1355	5.6e-20	0.8412698412698413

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd08023	GH16_laminarinase_like	4.87e-83	377	635	1	235	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
cd02182	GH16_Strep_laminarinase_like	9.20e-49	373	636	2	259	Streptomyces laminarinase-like, member of glycosyl hydrolase family 16. Proteins similar to Streptomyces sioyaensis beta-1,3-glucanase (laminarinase) present in Actinomycetales as well as Peziomycotina. Laminarinases belong to glycosyl hydrolase family 16 and hydrolyze the glycosidic bond of the 1,3-beta-linked glucan, a major component of fungal and plant cell walls and the structural and storage polysaccharides (laminarin) of marine macro-algae. Members of the GH16 family have a conserved jelly roll fold with an active site channel.
cd08024	GH16_CCF	5.05e-43	375	636	1	330	Coelomic cytolytic factor, member of glycosyl hydrolase family 16. Subgroup of glucanases of unknown function that are related to beta-GRP (beta-1,3-glucan recognition protein), but contain active site residues. Beta-GRPs are one group of pattern recognition receptors (PRRs), also referred to as biosensor proteins, that complexes with pathogen-associated beta-1,3-glucans and then transduces signals necessary for activation of an appropriate innate immune response. Beta-GRPs are present in insects and lack all catalytic residues. This subgroup contains related proteins that still contain the active site and are widely distributed in eukaryotes. Their structures adopt a jelly roll fold with a deep active site channel harboring the catalytic residues, like those of other glycosyl hydrolase family 16 members.
cd00413	Glyco_hydrolase_16	3.17e-34	379	635	1	210	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
COG2273	BglS	2.31e-31	370	720	38	342	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ASS68012.2	3.52e-172	292	1499	487	1658
ABJ15796.1	4.96e-172	282	1505	452	1654
ABX43741.1	2.51e-168	371	1662	59	1300
QGG54513.1	4.14e-150	370	1402	511	1528
BBF45016.1	1.97e-148	348	1411	34	1087

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VY0_A	3.72e-43	373	607	13	235	TheX-ray structure of endo-beta-1,3-glucanase from Pyrococcus furiosus [Pyrococcus furiosus],2VY0_B The X-ray structure of endo-beta-1,3-glucanase from Pyrococcus furiosus [Pyrococcus furiosus]
3ILN_A	2.20e-42	373	636	5	248	ChainA, Laminarinase [Rhodothermus marinus],3ILN_B Chain B, Laminarinase [Rhodothermus marinus]
6JH5_A	2.05e-41	373	636	4	232	Structureof Marine bacterial laminarinase [Aquimarina]
4CRQ_A	2.08e-41	373	636	3	231	Crystalstructure of the catalytic domain of the modular laminarinase ZgLamC mutant E142S [Zobellia galactanivorans],4CRQ_B Crystal structure of the catalytic domain of the modular laminarinase ZgLamC mutant E142S [Zobellia galactanivorans],4CTE_A Crystal structure of the catalytic domain of the modular laminarinase ZgLamC mutant E142S in complex with a thio-oligosaccharide [Zobellia galactanivorans],4CTE_B Crystal structure of the catalytic domain of the modular laminarinase ZgLamC mutant E142S in complex with a thio-oligosaccharide [Zobellia galactanivorans]
2HYK_A	2.96e-41	375	637	9	244	Thecrystal structure of an endo-beta-1,3-glucanase from alkaliphilic Nocardiopsis sp.strain F96 [Nocardiopsis sp. F96]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P23903	1.13e-59	374	636	424	680	Glucan endo-1,3-beta-glucosidase A1 OS=Niallia circulans OX=1397 GN=glcA PE=1 SV=1
P45798	5.12e-40	373	636	40	283	Beta-glucanase OS=Rhodothermus marinus OX=29549 GN=bglA PE=1 SV=1
C1IE32	2.83e-39	373	609	20	240	Glucan endo-1,3-beta-glucosidase OS=Cryptopygus antarcticus OX=187623 PE=1 SV=1
Q27082	2.38e-35	373	636	25	253	Clotting factor G alpha subunit OS=Tachypleus tridentatus OX=6853 PE=1 SV=1
Q8N0N3	8.51e-23	443	608	132	303	Beta-1,3-glucan-binding protein OS=Penaeus monodon OX=6687 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000380	0.998655	0.000414	0.000216	0.000166	0.000145

TMHMM  Annotations     

start	end
7	29