CAZyme Information: MGYG000002396_01851

Basic Information

• Species: Bifidobacterium bifidum
• Lineage: Bacteria; Actinobacteriota; Actinomycetia; Actinomycetales; Bifidobacteriaceae; Bifidobacterium; Bifidobacterium bifidum
• CAZyme ID: MGYG000002396_01851
• CAZy Family: GH101
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1931		206180.53	5.1666

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002396	2311342	Isolate	South Korea	Asia

• Gene Location: Start: 2173949; End: 2179744 Strand: -

Full Sequence      

MRKRVVSVALAVALAVAPLGVGSTASAAPLSASDLQTLALRSAASSNDANANDVATVADD
ASVNGWTIDRNTAKGGEILAAGTGDYAGWTHFKSTSANGNATSSSGYPAVAISGKTIDLT
RAGEFSIKVKSPQAGSANRFGFYLGYKDPGNALFLGYDKGGWFWQKYVGGNGDWYNGTRV
AAPAANAEITVNVSWTAAKVATLTIDGQKAFDVDYSSMTALTDKLAMKAGSYSGTSEVTD
VYFKDFTVGEVAKHNVTGKVVDASGAAIAGAEVVTGKNSATTAADGTFTLTGLAAGDYTL
TVSAEGYDDATKTVTVADGNASVGNITLNKSAEVATETLSTAAMDVRVKKNFPSVYDYTM
KKLDGKKMYGQPKDVRVITINGTDVTLKDSDVTFKKVSATEAQYTLNVKSGDKINAVVTV
QIKVVDNTLKLNVTKIVNKADDAKTEAEENPVQTIAFPNQSLISVRSGQDGAQFTGARMS
SDTARPGDTNFGITANTTVSNANDYTYGFVSGNGLSAGLWSNSEHDGTTVGNTVAGGARN
TRVLTSTQKVGKATSFGLGTAPWYYHRVVTDTKKRTYTVEETDMPKMAVAIAGDENEDGT
VNWEDGAIAYRDIMNNPYKSEEVPELVAWRIAMNFGSQAQNPFLTTLDNVKKVALNTDGL
GQSVLLKGYGNEGHDSGHPDYGDINTRAGGAADMNTLMEKGTEYGARFGVHVNASEMYPE
AKAFSEDMVRRNSSGGLSYGWNWLDQGIGIDGIYDLASGMRKSRFADLKSKVGDNMDFIY
LDVWGNNTSGAEDSWETRKMSQMINQNGWRMTTEWGAGNEYDATFQHWAADLTYGGSGMK
GENSQVMRFLRNHQKDSWVGDYPSYGQAANAPLLGGYSMKDFEGWQGRNDYAAYIRNLYT
HDVSTKFIQHFKVVRWVNSPLDATSVKDASVNNGNEQITLKDDHGNVVVLSRGSNDTNNG
AYRNRTITLNGITVASGAVSPGNSNTVKGTESYLLPWLWDVNTGKLVKSSDEKLYHWNTQ
GGTTEWTLPKDWQNLASVKVYQLTDQGKTNEKTVAVSGGKISLTAEAETPYVVTKGSEKQ
ISVKWSEGMHVVDAGFNGGQNTLKDNWAVSGTGKAEVEGTNNAMLRLTGDVKVSQKLTDL
TAGKRYAIYVGVDNRTNSPAKITVTNGTKVLATNETGKSIAKNYIKAYGHNTYSNTEGGS
SYFQNMYVWFVAPESGDVKVTLSHSGACDNTDHVYFDDVRVLENGYKGLTLNADGTLKTL
TNDFEDNAQGIWPFVVSGSEGVEDNRIHLSELHAPFTQAGWDVKKMDDVLDGKWSVKANG
LIQKGTLIYQTIPQNVKLEPGETYKVSFKYQSGSDDIYAIATGDGEYNASTVKLTNLKKA
LGEDGTAEFEITGSITGDSWFGIYSTSTAPDLQNTSDSAAAFGGYKDFVLDDLKVEHVAS
AERTKADAEAKLKEVKDTYNGKSGDYSAEVWTTYVNTVAEIEALIAKDKPDYTTAYNKAV
ALAEYMKNAPGDDSNDAYDVATDAYTVEAGSQQALSGGNEGPASLAQDGNAGTHWHTSWS
ANAVSAGTAWYQFNLNEPTTIDGLRYMARSGGANANGKIKKYKITLTLSDGTTKDVVTNG
TFTTTSGVWQKVKFDAVKNVTKVRITALETAGQSAGEVNTYASAAELRVTTVRDVPSTEV
KVNKCDLQNLYDDASALTEATYTADTWKVLVAKRDAAKKVLDDENATAHDVALAYQNLKD
AIAALEERVDTSKLAGLVADAEKLKESAYTKDSWAAFKKALDAAKAVLNNANATKADVDA
AYNALNAAMKALKPASSKPTPAPETTDKSKLQATIDQAKALDLSGYTKKSAQAVRDALAK
AQSVLADDNATQADIDAAQKALADAIAALEKADANGNAISKTGANVAVIGMAGMMLVAAA
GAVFIARKRAE

Enzyme Prediction     

EC	3.2.1.97

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH101	342	1075	0	0.9957567185289957
CBM32	1531	1667	1.9e-16	0.9354838709677419

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam12905	Glyco_hydro_101	2.72e-169	604	895	1	273	Endo-alpha-N-acetylgalactosaminidase. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by the S. pneumoniae protein Endo-alpha-N-acetylgalactosaminidase, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor.
cd14244	GH_101_like	5.18e-139	617	918	1	298	Endo-a-N-acetylgalactosaminidase and related glcyosyl hydrolases. This family contains the enzymatically active domain of cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins (EC:3.2.1.97). It has been classified as glycosyl hydrolase family 101 in the Cazy resource. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae and other commensal human bacteria is largely determined by their ability to degrade host glycoproteins and to metabolize the resultant carbohydrates.
pfam17974	GalBD_like	1.95e-109	1237	1424	1	190	Galactose-binding domain-like. Proteins containing a galactose-binding domain-like fold can be found in several different protein families, in both eukaryotes and prokaryotes. The common function of these domains is to bind to specific ligands, such as cell-surface-attached carbohydrate substrates for galactose oxidase and sialidase, phospholipids on the outer side of the mammalian cell membrane for coagulation factor Va, membrane-anchored ephrin for the Eph family of receptor tyrosine kinases, and a complex of broken single-stranded DNA and DNA polymerase beta for XRCC1. The structure of the galactose-binding domain-like members consists of a beta-sandwich, in which the strands making up the sheets exhibit a jellyroll fold.
pfam18080	Gal_mutarotas_3	1.73e-75	339	603	1	243	Galactose mutarotase-like fold domain. This domain is found in endo-alpha-N-acetylgalactosaminidase present in Streptococcus pneumoniae. Endo-alpha-N-acetylgalactosaminidase is a cell surface-anchored glycoside hydrolase involved in the breakdown of mucin type O-linked glycans. The domain, known as domain 2, exhibits strong structural similarlity to the galactose mutarotase-like fold but lacks the active site residues. Domains, found in a number of glycoside hydrolases, structurally similar to domain 2 confer stability to the multidomain architectures.
pfam17451	Glyco_hyd_101C	1.26e-40	901	1049	1	111	Glycosyl hydrolase 101 beta sandwich domain. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by a S. pneumoniae protein, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor. This domain represents C-terminal the beta sandwich domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AXM92235.1	0.0	1	1931	1	1931
ADO52490.1	0.0	1	1931	1	1931
VEG14253.1	0.0	1	1931	1	1931
QTQ17692.1	0.0	1	1931	1	1931
ADP35390.1	0.0	1	1931	1	1931

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6QEV_B	0.0	334	1507	7	1188	EngBFDARPin Fusion 4b B6 [Bifidobacterium longum]
6QFO_A	0.0	334	1509	7	1190	EngBFDARPin Fusion 9b 3G124 [Bifidobacterium longum]
6QEP_A	0.0	334	1507	7	1188	EngBFDARPin Fusion 4b H14 [Bifidobacterium longum]
6SH9_B	0.0	334	1507	7	1188	EngBFDARPin Fusion 4b D12 [Bifidobacterium longum subsp. longum JCM 1217]
6QFK_A	0.0	334	1507	7	1188	EngBFDARPin Fusion 4b G10 [Bifidobacterium longum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q2MGH6	9.74e-318	89	1667	147	1622	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=SP_0368 PE=1 SV=1
Q8DR60	2.13e-316	89	1705	147	1670	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=spr0328 PE=1 SV=1
A9WNA0	2.09e-130	338	1529	51	1130	Putative endo-alpha-N-acetylgalactosaminidase OS=Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235) OX=288705 GN=RSal33209_1326 PE=3 SV=2
E8MGH9	3.80e-19	1559	1919	1531	1923	Beta-L-arabinobiosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA2 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000339	0.998887	0.000189	0.000217	0.000187	0.000173

TMHMM  Annotations     

start	end
5	27
1904	1926