CAZyme Information: MGYG000002453_01493

Basic Information

• Species: Akkermansia muciniphila_B
• Lineage: Bacteria; Verrucomicrobiota; Verrucomicrobiae; Verrucomicrobiales; Akkermansiaceae; Akkermansia; Akkermansia muciniphila_B
• CAZyme ID: MGYG000002453_01493
• CAZy Family: GH97
• CAZyme Description: Retaining alpha-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
644		71410.63	7.347

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002453	3096868	Isolate	China	Asia

• Gene Location: Start: 139613; End: 141547 Strand: +

Full Sequence      

MIVKSLIIFSNMAGCLLPGSGQAGELHASLQSPDGRLSWMLHADNRAAHSLKKGNRTVLE
PSGLGIVVNGKDLAGGITGWSVKKVKDNIHDTFETRGKYPSASVHFNEYVVSGDNSGLRL
RARVFNNGVAFRYEWTEDMQKAPVLNIGEEKTSFAFPEKAVLWTQDSASALGPCEGVWSP
SRIVDFKKDPGNPRSHVRTMPVAAELPGGGFALIQEAANFNRQWSGIKFSLQDGACRTVY
FQDPEGFSAPSSAEMPWRVILVNDDLNGLVRNDVIPSLAPEPDRNLFPEGAKTPWIKPGR
STWTWWDRGNVREEDQYAFVDMASEFGWEYHLVDEGWKKWGKTVPESMDKVAQLASYAAG
KQVGIWVWVRWSDVNNPANDWESMRSFFRSLAKTGIKGIKIDFMDSASRERLAFYDAVAE
NLAKNKLMVNFHGANTPTGEERSWPHEMSREGIYGGEQNIWAAIGGQHYCALPFTRLVSG
HADFTGGYFGHGPKLRGSSWPLQMAANIIYTSPVLHWVSNPADMEAAFPKGSPEREAVRN
IPSVWDETIVLPPSAIGECAAFARRSGDQWYIALMNGDGRERTVSIPLDFLNRNTAYRAT
ILRDLPEKNDGWKVENRELHSGSTLSFTMRVKGGGIARLVPAGK

Enzyme Prediction     

No EC number prediction in MGYG000002453_01493.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH97	27	639	7.2e-146	0.9778129952456418

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam10566	Glyco_hydro_97	8.41e-56	293	516	2	256	Glycoside hydrolase 97. This domain is the catalytic region of the bacterial glycosyl-hydrolase family 97. This central part of the GH97 family protein sequences represents a typical and complete (beta/alpha)8-barrel or catalytic TIM-barrel type domain. The N- and C-terminal parts of the sequences, mainly consisting of beta-strands, form two additional non-catalytic domains. In all known glycosidases with the (beta-alpha)8-barrel fold, the amino acid residues at the active site are located on the C-termini of the beta-strands.
pfam14508	GH97_N	1.22e-41	32	282	1	235	Glycosyl-hydrolase 97 N-terminal. This N-terminal domain of glycosyl-hydrolase-97 contributes part of the active site pocket. It is also important for contact with the catalytic and C-terminal domains of the whole.
pfam14509	GH97_C	1.15e-26	545	640	1	97	Glycosyl-hydrolase 97 C-terminal, oligomerization. Glycosyl-hydrolase-97 is made up of three tightly linked and highly conserved globular domains. The C-terminal domain is found to be necessary for oligomerization of the whole molecule in order to create the active-site pocket and the Ca++-binding site.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUY59643.1	0.0	1	644	1	644
QHV75690.1	0.0	1	644	1	644
QHV63320.1	0.0	1	644	1	644
QWP02002.1	0.0	1	642	1	642
QWP20638.1	0.0	1	642	1	642

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5XFM_A	9.19e-50	30	596	19	604	Crystalstructure of beta-arabinopyranosidase [Bacteroides thetaiotaomicron],5XFM_B Crystal structure of beta-arabinopyranosidase [Bacteroides thetaiotaomicron],5XFM_C Crystal structure of beta-arabinopyranosidase [Bacteroides thetaiotaomicron],5XFM_D Crystal structure of beta-arabinopyranosidase [Bacteroides thetaiotaomicron]
3A24_A	6.48e-47	32	604	6	602	Crystalstructure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron],3A24_B Crystal structure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron]
5E1Q_A	6.71e-46	32	604	20	616	Mutant(D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482],5E1Q_B Mutant (D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482]
5HQC_A	3.42e-34	28	634	1	653	AGlycoside Hydrolase Family 97 enzyme R171K variant from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]
5HQ4_A	4.59e-34	28	634	1	653	AGlycoside Hydrolase Family 97 enzyme from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8],5HQA_A A Glycoside Hydrolase Family 97 enzyme in complex with Acarbose from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
D7CFN7	7.35e-62	51	634	63	615	Probable retaining alpha-galactosidase OS=Streptomyces bingchenggensis (strain BCW-1) OX=749414 GN=SBI_01652 PE=3 SV=1
Q8A6L0	5.17e-47	15	604	10	623	Retaining alpha-galactosidase OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_1871 PE=1 SV=1
G8JZS4	1.92e-28	30	621	25	697	Glucan 1,4-alpha-glucosidase SusB OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=susB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000598	0.621481	0.377085	0.000353	0.000260	0.000204

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002453_01493.