CAZyme Information: MGYG000002941_00734

Basic Information

• Species: Actinomyces graevenitzii
• Lineage: Bacteria; Actinobacteriota; Actinomycetia; Actinomycetales; Actinomycetaceae; Actinomyces; Actinomyces graevenitzii
• CAZyme ID: MGYG000002941_00734
• CAZy Family: GH35
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1607	MGYG000002941_14|CGC1	166083.75	6.2251

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002941	1921862	MAG	United States	North America

• Gene Location: Start: 7215; End: 12038 Strand: +

Full Sequence      

MPRKLAKRAATLAAVVGMALAGGALPISVAQAASAAPPAKYATADITFPGNDGKPHKVTF
DKNSFMVDGQRLNVWSGEIHYWRAPDVNSWRDLFQKMRANGYNAVSLYFFWGLHQSKEGG
PFDFSKGTIKDLDLLLTMAQEEGLYVIARPGPYVNAEISMGGLPAWMTNYSGGLRTTDPK
VLAASKAWLHAFNQIASKHLVTKGGGSVLLYQVENELLSDNAARGAFLKSLVSQVKSDGI
DVPLFHNDYGLGGRFKNTSLYGTDFYAYDKYPVGFNCSAGRNRIDDSEATFRSYAPNSPH
FITESQGGAFTPWGASYNASDCYTYTDEGFTRQWGVHNIGNGVTAFNYYMAYGGTNWGWT
GAPASGFTSYDYGAGITEDRTLTPKASVQKETGYYTKAVPELSSMNPVQAPSAVVESGSN
VKIYARQAETGQGSVTGNGVRTYAMRLANSNDTTETTFTTALSLGKPRGATTTGFSHDDR
NSAITYTGSWSQVADSSAASGTLTRTKTVGDTASFTFTGTGVRLITATGTDHGYFKVSID
GGEAQLVTSAHVDTEQNKPTQLEAYRIENLSPGQHTIKVENVGFDGANVVDIDAFDVLGS
TAAQAPIYNDSDTSFFTFTGSWQHATGKNWTQGDHSGDETFSKTAGDSYTFTFTGVGFDI
IAPFSSNHGSATVTVDGQEVGQTKEDVTSEATPQQTVFSWRAEKGAETGARGGSHGAEAG
AGTDKAGTQGAEAGAQGGSTDAGTEGGSQGAEAGAQGGEAAAQSGANGAGTDKAGATASQ
AAKPATHTVKVTVDGKAFSGSQDTFVSLDAVKVYPNAQSLPGENNDEADGTISWKRIPQK
EGTTLTLHGRDAIMLTADKQMGGHELYYTTSQMFGATLNSAGGSLQYLTGTRGDSGETVL
HYTTQPQVSAPEGVEQTWDAATGQLRLNYSYSASPQQIIIKDGDKVLSLRIIDRTTAQTT
WLLDGTRNGKLSSVAVEGVELARTAKFTGKDTLQLTGSVSAESNVRVIAPAGINHVGFNG
GSLNPASATGSAGAGTNSATGSAGNAGAAKFTGTAGAAGAASAGNADAAGAGTNSATGTG
DVGVFTGKVPGPAAVASQQLSFVKSTDYAEAKATYDDSKWKVAADTQAANPRFQGPGEYS
HTVLDSNHYGFYEGSVWYRGTFTATKADPYLYLQGNGGSGVPSQGKNPAFMQVWVNGVYA
GAYDAAGNWAKVNVPAGTVKSGDKVTVAVLVNNLGQNLDWSDDGLSKQNRGLFDVAIEGS
AATWKIHGADADFAAKATTNPSGTLYNNGGLGGEKAGFHMPGFDDSKWAKADNLHSQAGV
TWYRARVKLNLPANQDTAFRLDINSSRFSSLGDRAQATLFVNGWNTGVYIGDRGPQTSFT
IPAGFLNPNGENVISIAVAAKEDGMGPDSVTLRAAHSTTKPALGSTPAPTGQPTTSPQPT
VAPTAAPTVVPTATAVPTVAPTVAPTATAAPTGQPTVAPTVKPTGTASPTAQPTVSPTGA
PTANPTAAPTGQPTVAPQPTAGPTASSAPGQPTAGPGQPTASPTASTHPTAAPGQPGKPS
TAPAPDGTREPMAGEQLPRTGVNLALGLGAAAALAGGLVLLRRRRAL

Enzyme Prediction     

No EC number prediction in MGYG000002941_00734.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH35	65	391	1.6e-84	0.9837133550488599

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam01301	Glyco_hydro_35	1.92e-80	64	385	1	307	Glycosyl hydrolases family 35.
PLN03059	PLN03059	3.31e-25	58	451	30	393	beta-galactosidase; Provisional
COG1874	GanA	4.76e-22	58	349	1	268	Beta-galactosidase GanA [Carbohydrate transport and metabolism].
smart01029	BetaGal_dom2	9.22e-20	828	960	57	182	Beta-galactosidase, domain 2. This is the second domain of the five-domain beta-galactosidase enzyme that altogether catalyses the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and trans-glycosylation. This domain is made up of 16 antiparallel beta-strands and an alpha-helix at its C terminus. The fold of this domain appears to be unique. In addition, the last seven strands of the domain form a subdomain with an immunoglobulin-like (I-type Ig) fold in which the first strand is divided between the two beta-sheets. In penicillin spp this strand is interrupted by a 12-residue insertion which forms an additional edge-strand to the second beta-sheet of the sub-domain. The remainder of the second domain forms a series of beta-hairpins at its N terminus, four strands of which are contiguous with part of the Ig-like sub-domain, forming in total a seven-stranded antiparallel beta-sheet. This domain is associated with family Glyco_hydro_35, which is N-terminal to it, but itself has no metazoan members.
pfam13364	BetaGal_dom4_5	5.46e-17	1293	1408	2	111	Beta-galactosidase jelly roll domain. This domain is found in beta galactosidase enzymes. It has a jelly roll fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
VEG55570.1	0.0	40	1435	61	1345
QQM67568.1	0.0	40	1433	60	1339
QWW19060.1	0.0	4	1432	3	1318
AOZ72117.1	0.0	46	1437	58	1309
QGH69595.1	0.0	48	1412	67	1319

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3OG2_A	2.22e-63	58	423	26	396	ChainA, Beta-galactosidase [Trichoderma reesei],3OGR_A Chain A, Beta-galactosidase [Trichoderma reesei],3OGS_A Chain A, Beta-galactosidase [Trichoderma reesei],3OGV_A Chain A, Beta-galactosidase [Trichoderma reesei]
1TG7_A	9.06e-61	58	407	6	361	Nativestructure of beta-galactosidase from Penicillium sp. [Penicillium sp.],1XC6_A Native Structure Of Beta-Galactosidase from Penicillium sp. in complex with Galactose [Penicillium sp.]
4IUG_A	1.57e-59	58	423	46	416	Crystalstructure of beta-galactosidase from Aspergillus oryzae in complex with galactose [Aspergillus oryzae]
5IFP_A	4.87e-57	58	407	46	400	StructureOf Beta-galactosidase From Aspergillus Niger [Aspergillus niger CBS 513.88]
5IFT_A	1.14e-56	58	407	46	400	STRUCTUREOF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 3-b-Galactopyranosyl glucose [Aspergillus niger CBS 513.88],5IHR_A Structure Of E298q-beta-galactosidase From Aspergillus Niger In Complex With Allolactose [Aspergillus niger CBS 513.88],5JUV_A STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 6-b-Galactopyranosyl galactose [Aspergillus niger CBS 513.88],5MGC_A STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 4-Galactosyl-lactose [Aspergillus niger CBS 513.88],5MGD_A STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 6-Galactosyl-lactose [Aspergillus niger CBS 513.88]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
B0Y752	2.38e-66	51	402	25	383	Probable beta-galactosidase C OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=lacC PE=3 SV=1
Q4WNE4	2.38e-66	51	402	25	383	Probable beta-galactosidase C OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=lacC PE=3 SV=1
A1DM65	2.40e-65	51	402	25	383	Probable beta-galactosidase C OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=lacC PE=3 SV=1
B6QHA9	8.70e-62	58	458	30	429	Probable beta-galactosidase C OS=Talaromyces marneffei (strain ATCC 18224 / CBS 334.59 / QM 7333) OX=441960 GN=lacC PE=3 SV=1
B8N2I5	1.79e-61	51	402	25	383	Probable beta-galactosidase C OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=lacC PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000562	0.997903	0.000239	0.000798	0.000277	0.000216

TMHMM  Annotations     

start	end
12	34
1582	1601