CAZyme Information: MGYG000002990_00373

Basic Information

• Species: Allisonella histaminiformans
• Lineage: Bacteria; Firmicutes_C; Negativicutes; Veillonellales; Dialisteraceae; Allisonella; Allisonella histaminiformans
• CAZyme ID: MGYG000002990_00373
• CAZy Family: GT9
• CAZyme Description: Lipopolysaccharide core heptosyltransferase RfaQ

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
352	MGYG000002990_2|CGC1	39165.25	7.8516

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002990	1615172	MAG	United States	North America

• Gene Location: Start: 75347; End: 76405 Strand: +

Full Sequence      

MEGKKIDLNNKTIVVMYMLYFGDMVSITPFLEVLRREAKGSKIVLVIDSRFKESVQYNPN
VDELIEVDRHGKDRSLSACWKIGKKIRQMHPDILITLHGTSRTSLMALAMKPGFWTGEPG
TRMDSLFMDYPVIIETYNSHAVDKYLRVLSLLGVENLTHHGMRTYTCEEWDRRADEFFAG
EGIKEGAKLVGLSVGSSTPEKNWPAENFGKVAEHFAGRGMIPVFFGVKSELPLIKKAVQE
VKHPYVIAAGKLSMGEFMAAASHCSLFFTNDSGPMYVADSRGVPTIAMFGPSNAKFHHPL
GKYSRAISSWDMPMGPEHVNKNIKSGNYVPLDKIAVEEVIAAGEEALRNAGC

Enzyme Prediction     

No EC number prediction in MGYG000002990_00373.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT9	80	307	3.1e-41	0.8888888888888888

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd03789	GT9_LPS_heptosyltransferase	4.97e-53	12	342	1	274	lipopolysaccharide heptosyltransferase and similar proteins. Lipopolysaccharide heptosyltransferase (2.4.99.B6) is involved in the biosynthesis of lipooligosaccharide (LOS). Lipopolysaccharide (LPS) is a major component of the outer membrane of gram-negative bacteria. LPS heptosyltransferase transfers heptose molecules from ADP-heptose to 3-deoxy-D-manno-octulosonic acid (KDO), a part of the inner core component of LPS. This family also contains lipopolysaccharide 1,2-N-acetylglucosaminetransferase EC 2.4.1.56 and belongs to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.
COG0859	RfaF	5.51e-45	11	349	2	332	ADP-heptose:LPS heptosyltransferase [Cell wall/membrane/envelope biogenesis].
pfam01075	Glyco_transf_9	1.68e-30	80	313	2	235	Glycosyltransferase family 9 (heptosyltransferase). Members of this family belong to glycosyltransferase family 9. Lipopolysaccharide is a major component of the outer leaflet of the outer membrane in Gram-negative bacteria. It is composed of three domains; lipid A, Core oligosaccharide and the O-antigen. All of these enzymes transfer heptose to the lipopolysaccharide core.
PRK10422	PRK10422	2.03e-13	7	347	2	343	lipopolysaccharide core biosynthesis protein; Provisional
PRK10916	PRK10916	2.55e-08	199	307	193	305	ADP-heptose--LPS heptosyltransferase RfaF.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BBK25206.1	1.63e-187	2	350	4	354
AXL20515.1	3.49e-94	6	349	2	344
ALG42175.1	5.80e-93	6	349	2	344
AVO27634.1	5.80e-93	6	349	2	344
CCC73399.1	5.80e-93	6	349	2	344

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3TOV_A	3.40e-76	7	350	5	348	Thecrystal structure of the glycosyl transferase family 9 from Veillonella parvula DSM 2008 [Veillonella parvula DSM 2008],3TOV_B The crystal structure of the glycosyl transferase family 9 from Veillonella parvula DSM 2008 [Veillonella parvula DSM 2008]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000059	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
13	35