CAZyme Information: MGYG000003136_01440

Basic Information

• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Streptococcus
• CAZyme ID: MGYG000003136_01440
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1513		167982.62	6.2868

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003136	1781350	MAG	United States	North America

• Gene Location: Start: 213; End: 4754 Strand: +

Full Sequence      

MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLFAGNIAGESVVNADETPITRTAEKPKEE
NPIVEEKLDQALETKNVVESTAQKQPSATEAIASEKKEDEAVTPKEEKASSKSDEKAPKI
ESQASSQEKPLKEDVKAVTNEEVNQMIEDRKVNFNQNWHFKLNANSKEAIKPDADVSTWK
KLDLPHDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTFKLDEKDLKKNVRLTFDGVYMDS
QVYVNGQLVGHYPNGYNQFSYDITKYLNKDGRENVIAVHAVNKQPSSRWYSGSGIYRDVT
LQVTDKVHVEKNGTTILTPKLEQQQHSKVETHVTSKIVNTDDKDHQLVAEYQIVERGGQA
VTGLVRTESRTLKAHEVTSLDAILEVERPKLWTVLNDKPALYELVTRVYRDGQLVDAKKD
LFGYRYYNWTPNEGFSLNGERIKFHGVSLHHDHGALGAEENYKAEYRRLKQMKEMGVNSI
RTTHNPASPQTLQIAAELGLLVQEEAFDTWYGGKKPYDYGRFFEKDATHPEAKKGEKWSD
YDLRTMVERDKNNPAVFMWSIGNEIGEANGDPHSLATVKRLVKVIKDVDKTRYVTMGADK
FRFGNGSGGHEKIADELDAVGFNYSEDNYKALRAKHPKWLIYGSETSSATRTRGSYFHPE
REWVQSNQSWRNYEQSDYGNDRVGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPW
HNQNNTPVKSSYFGIVDTAGIPKNDFYLYQSQWVSAKKKPMVHLLPHWNWENKELIDNVA
DAENKIPVRAYSNAASVELFLNGQSLGEKKFNKKQTSDGRTYQEGANANELYLEWKVAYQ
PGTLEAIARDESGKEIARDKITTAGKPAAVRLIREEHAIAADGKDLTYIYYEIVDSQGNV
VPTANNLVRFQLHGQGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAG
KFTLTAHSDLLKSSQVTVFTGKTEGQEKTVLGTEVARVRTLIGKEPKMPKTVGFVYSDGS
REKLPVTWSQVDVSQAGVVTAKGTANGREVEARVEVLAIAKELQTVKRVAPGADLNTVDK
YVSILVTDGSVQEYEVDSWEIAEADKAKLSVAGSRIQMTGQLAGETIHATLVVEEGKAAA
PVVPTVTVGGEVVTGLTSQQPMQYRTLAYGAQLPEVTASAENADVTVLQASAANGMRASI
FVQSKDGGPLQTYAIQFLEEAPKIAHLSLQVEQADGLKEDQTVNLSVRAHYQDGTQAVFP
AYKVSFSTSGEGEVAVRKGMLELHKSGALTLKAEYEGATGQINLTIQANTEKKIAQSIRP
VNVVTDLHQEPTLPSTVTVEYDKGFPKAHKVTWQAIPKEKLDHYQSFEVLGKVEGIDLEA
RAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVTWDAIRPEQYAKEGV
FTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPTDPVSHVNDKL
ISYNNQPANRWTN

Enzyme Prediction     

No EC number prediction in MGYG000003136_01440.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	127	890	7.1e-115	0.7992021276595744

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	0.0	124	966	1	803	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	1.90e-34	158	595	19	446	beta-D-glucuronidase; Provisional
pfam18565	Glyco_hydro2_C5	7.36e-34	871	977	2	103	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.
PRK10340	ebgA	6.40e-31	153	594	43	472	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam02837	Glyco_hydro_2_N	1.66e-25	155	301	5	165	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QQQ35963.1	0.0	1	1513	1	1505
AYF95976.1	0.0	1	1513	1	1513
VFI34789.1	0.0	1	1513	1	1513
VFH58268.1	0.0	1	1513	1	1513
VFH83902.1	0.0	1	1513	1	1513

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4CUC_A	0.0	137	985	1	849	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA. [Streptococcus pneumoniae TIGR4]
4CU6_A	0.0	137	985	1	849	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU7_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU8_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4]
4YPJ_A	3.90e-268	149	980	7	807	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
7CWD_A	4.15e-259	150	980	2	801	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
7NIT_A	1.33e-198	148	1057	29	959	ChainA, Beta-galactosidase [Bifidobacterium bifidum],7NIT_B Chain B, Beta-galactosidase [Bifidobacterium bifidum],7NIT_C Chain C, Beta-galactosidase [Bifidobacterium bifidum],7NIT_D Chain D, Beta-galactosidase [Bifidobacterium bifidum],7NIT_E Chain E, Beta-galactosidase [Bifidobacterium bifidum],7NIT_F Chain F, Beta-galactosidase [Bifidobacterium bifidum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	6.24e-176	154	974	47	842	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	5.36e-107	153	924	50	768	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	7.71e-53	151	892	53	761	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
T2KN75	3.26e-49	153	978	30	793	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1
P77989	3.29e-49	152	968	6	725	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.014739	0.805318	0.178631	0.000617	0.000388	0.000306

TMHMM  Annotations     

start	end
17	39