CAZyme Information: MGYG000003162_00164

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Ruminococcus_D
• CAZyme ID: MGYG000003162_00164
• CAZy Family: GH5
• CAZyme Description: Endoglucanase 1

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
386		43401.61	4.2708

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003162	1479112	MAG	United States	North America

• Gene Location: Start: 330; End: 1490 Strand: -

Full Sequence      

MRIKKLAAAAIAAVMCLSCITACGKNDDSSSAAGSSESSDGKKEVNMEIRDISSWELVKE
MSIGWNLGNTMDAVGSTGLASETAWQPVTTNEGMATLLKDTGFNVMRVPVSWGDHMDDEY
KVDEEWMARVHEIVDYGINNGLFVILNTHHEEWYFPTAENKDEDIKQLTALWSQIAEEFK
GYDEHLIFEGLNEPRCRNTPMEWTGGDADSRAIVAEYAKAFYETVRASGGNNEKRHLMLT
GYAASSNRNCLEQVWLPENDDKVIVSVHAYLPYSLALDTKGTDKWDANNTSEIDNFFFNL
DELFIQKQIPVIIGEFGTVNKDNLEDRIACAEYYVGKGAEYGIPMIWWDNNAIVGNGENF
GLMDRNMMPEWKFPELVDVLIESSKR

Enzyme Prediction     

EC	3.2.1.4	3.2.1.73

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH5	81	351	2.1e-93	0.9927536231884058

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam00150	Cellulase	1.15e-67	74	352	8	270	Cellulase (glycosyl hydrolase family 5).
COG2730	BglC	8.75e-36	56	386	46	396	Aryl-phospho-beta-D-glucosidase BglC, GH1 family [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADU20585.1	4.05e-149	1	378	1	392
CCO06082.1	2.19e-148	1	383	1	381
CAA38693.1	1.83e-143	1	379	1	395
AAA26469.1	7.68e-142	53	378	68	392
CBL17440.1	3.70e-118	3	382	6	374

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3AYR_A	5.49e-113	48	380	14	356	GH5endoglucanase EglA from a ruminal fungus [Piromyces rhizinflatus]
3AYS_A	4.43e-112	48	380	14	356	GH5endoglucanase from a ruminal fungus in complex with cellotriose [Piromyces rhizinflatus]
6WQP_A	6.46e-101	48	382	10	354	GH5-4broad specificity endoglucanase from Ruminococcus champanellensis [Ruminococcus champanellensis],6WQP_B GH5-4 broad specificity endoglucanase from Ruminococcus champanellensis [Ruminococcus champanellensis],6WQV_A GH5-4 broad specificity endoglucanase from Ruminococcus champanellensis with bound cellotriose [Ruminococcus champanellensis],6WQV_B GH5-4 broad specificity endoglucanase from Ruminococcus champanellensis with bound cellotriose [Ruminococcus champanellensis],6WQV_C GH5-4 broad specificity endoglucanase from Ruminococcus champanellensis with bound cellotriose [Ruminococcus champanellensis],6WQV_D GH5-4 broad specificity endoglucanase from Ruminococcus champanellensis with bound cellotriose [Ruminococcus champanellensis]
6Q1I_A	1.04e-97	53	385	13	354	GH5-4broad specificity endoglucanase from Clostrdium longisporum [Clostridium longisporum],6Q1I_B GH5-4 broad specificity endoglucanase from Clostrdium longisporum [Clostridium longisporum]
6MQ4_A	1.48e-96	49	384	7	349	ChainA, cellulase [Acetivibrio cellulolyticus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P23661	3.66e-144	1	379	1	395	Endoglucanase B OS=Ruminococcus albus OX=1264 GN=celB PE=3 SV=1
P16216	1.54e-142	53	378	68	392	Endoglucanase 1 OS=Ruminococcus albus OX=1264 GN=Eg I PE=1 SV=1
P23660	2.66e-102	48	385	21	364	Endoglucanase A OS=Ruminococcus albus OX=1264 GN=celA PE=1 SV=1
Q12647	1.53e-100	49	382	20	361	Endoglucanase B OS=Neocallimastix patriciarum OX=4758 GN=CELB PE=2 SV=1
P54937	8.47e-96	53	385	38	379	Endoglucanase A OS=Clostridium longisporum OX=1523 GN=celA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000000	0.000001	1.000081	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003162_00164.