CAZyme Information: MGYG000003512_01131

Basic Information

• Species: UBA11524 sp900769075
• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; CAG-74; UBA11524; UBA11524 sp900769075
• CAZyme ID: MGYG000003512_01131
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1127		124560.54	5.3476

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003512	2777574	MAG	Fiji	Oceania

• Gene Location: Start: 26800; End: 30183 Strand: +

Full Sequence      

MECLFNDGWQFVKLQNGSVLSDAENAAWVSVDLPHDFLISQENNLYESADGWYRRSLNVP
EAWLGRDVILRFDGVYMDCDVLLNGEVICTHHNGYTCFDAVLTARLRAGENVIHVHIRHR
SPNSRWYSGAGIFRDVTLHVLPPRHIALDGTYVTTRRDGDVWTLTVETELTGAGENRPIV
HRLLDDRGLVCETAVRPESDHTAAVLTVRNPRLWQVGEGGCYTLETSFGEQVIRENVGFR
EVEITTAHGLFVNGGRVKLHGVCLHHDLGCLGSAFNTAAARRQLLAMKEMGVNSLRTSHN
PPAKPVMNLCDALGLLVVDEAFDMWQHPKTPYDYARFFDADVESDVAAWVRRDRNHPSLL
MWSIGNEIGDTNNDPEQAGKVTRMLCENVRRHDPRGNGATTIGSNYMPWAGAQHCAQFVE
AVGYNYGEKLYEKHHAAHPEWAIYGSETASSVSSRGVYRFPMDAPILADDDLQCSSLGNS
NTSWGTQNMAKMIVEDLNCEYSLGQYLWSGIDYLGEPTPYHTRSSYFGMMDTAVFPKDNW
YLFKALWTDAPMVHIGVAWDWNPGQMIDVPVMTNGAAVELFLNGASLGRQAVDVRDGDKC
RPVWRVPFAAGELRAVAYKADGAVMAETARHTPGDSAKILLSAEQTALHATGTDLAFITI
SMADEQGHPVENACDRVHVRVTGQGMLMGLDNGDSTDRDGYKTTTRRLFSGKLLAVVGDL
GEEGSVRIEVTSPGKEPAALVLPVLPGVAAGAKRTVPLCREEPMPDEIPVRKIELIPLEE
KKLKTDRKSVSFRIRTYPENAMPQALSFRITNAKGIDSPCAAYAVEGDVVTVTGLGDGTV
YLRACASNGYEHPRIISQQDIRIEGLGQPSLDPYAFVSAGLYTLSYGDLTSGNEQGVAFA
RDGESMAGFTRVDFGPVGSDEITLPIFALDSTHYDLTLWDGDPREGGEILGVLPYQKPSR
WNVYQPETYTLPRRLTGIHTLCFTMDRKIHLKGFSFTRQSRAWMNLRALQADAVYGDSFT
RGEEGILDIGNNVSLVFGNMDFGDASRATLTIDGSTPLKENPITVRFQNEAGESLTALAQ
FKGTRRGCQSFRVDVLPGMCTVTFVFLPGCRFDFMAFRFSPDVERPV

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	5	552	9.4e-104	0.6103723404255319

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	3.21e-65	10	449	11	490	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	8.74e-48	9	394	19	441	beta-D-glucuronidase; Provisional
PRK10340	ebgA	3.43e-39	51	369	111	451	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	1.63e-33	51	369	122	464	beta-galactosidase.
pfam18565	Glyco_hydro2_C5	3.87e-29	641	741	3	103	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QTE68512.1	0.0	1	1120	1	1123
QUC68071.1	0.0	1	1119	1	1116
QTE71175.1	0.0	1	1119	1	1117
QTE75139.1	0.0	1	1119	1	1117
QUA53909.1	0.0	1	1119	1	1113

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4YPJ_A	1.63e-152	5	734	12	797	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
7CWD_A	2.39e-148	5	734	6	791	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
4CU6_A	2.07e-135	5	732	18	832	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU7_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU8_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4]
4CUC_A	5.64e-135	5	732	18	832	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA. [Streptococcus pneumoniae TIGR4]
5T98_A	1.13e-132	5	744	30	820	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	2.33e-142	5	740	47	844	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	4.99e-76	1	712	47	788	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	1.24e-44	6	617	57	702	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
T2KN75	3.40e-36	5	769	31	826	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1
Q56307	6.40e-35	47	370	108	444	Beta-galactosidase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=lacZ PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000074	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003512_01131.