CAZyme Information: MGYG000004109_01499

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia
• CAZyme ID: MGYG000004109_01499
• CAZy Family: GH36
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
537		61103.42	5.7935

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004109	2073923	MAG	United Kingdom	Europe

• Gene Location: Start: 6883; End: 8496 Strand: -

Full Sequence      

MNIRYPDYIAAYGENVSFDAKRAKLLAKDDVRIELLLADSALKIFLLADKTPMRYIKLRW
NFAENEKRNEDVKVYGDVWERSYGDIEWRGIEPWRTMPWVCAASNGSDLNEDVSGRCTSC
FGVKVRPHAMCFWQYDTCGVTLWLDVRCGGSGVILGGRKAELCTVLFADYREVSAFSALK
AFYKTLCDAPLSCGHKVYGANNWYYAYGETSHAETVRDASLLAEMTEGLSNRPYSVLDDG
WQKNPCDAPWDVLREGKFQDMKAVADEVKSKNVRPGIWFRPLRDENFEVFPAESELRLAR
NKKFLDPSHPDVLAYVAKTVNMICDWGFELIKHDFSTFDAIGFWGLQRYGTPFAEDGWSY
FDRSKTTAEVILGLNETIYNAAKGRALILGCNVMGHLAAGLVHLNRTGDDTSGKDWHRMV
KFGVNALAFRMLHHGAFYESDADCVGFMGIMDHKYNKQWLKAVACSGTPLFVSPNPDVLT
AKERKDIEEAYRINSVQSDTLIPLDWMENAAPEHWLLNGKHVKFDWYPEDGVDFFFI

Enzyme Prediction     

No EC number prediction in MGYG000004109_01499.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd14791	GH36	3.62e-33	197	490	3	293	glycosyl hydrolase family 36 (GH36). GH36 enzymes occur in prokaryotes, eukaryotes, and archaea with a wide range of hydrolytic activities, including alpha-galactosidase, alpha-N-acetylgalactosaminidase, stachyose synthase, and raffinose synthase. All GH36 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. GH36 members are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.
COG3345	GalA	5.02e-06	199	349	295	462	Alpha-galactosidase [Carbohydrate transport and metabolism].
pfam02065	Melibiase	2.38e-04	201	334	46	190	Melibiase. Glycoside hydrolase families GH27, GH31 and GH36 form the glycoside hydrolase clan GH-D. Glycoside hydrolase family 36 can be split into 11 families, GH36A to GH36K. This family includes enzymes from GH36A-B and GH36D-K and from GH27.
cd06592	GH31_NET37	0.001	233	281	33	80	glucosidase NET37. NET37 (also known as KIAA1161) is a human lamina-associated nuclear envelope transmembrane protein. A member of the glycosyl hydrolase family 31 (GH31) , it has been shown to be required for myogenic differentiation of C2C12 cells. Related proteins are found in eukaryotes and prokaryotes. Enzymes of the GH31 family possess a wide range of different hydrolytic activities including alpha-glucosidase (glucoamylase and sucrase-isomaltase), alpha-xylosidase, 6-alpha-glucosyltransferase, 3-alpha-isomaltosyltransferase and alpha-1,4-glucan lyase. All GH31 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein.
cd14790	GH_D	0.008	203	283	8	84	Glycoside hydrolases, clan D. This group of glycosyl hydrolase families is comprised of glycosyl hydrolase family 31 (GH31), family 36 (GH36), and family 27 (GH27). These structurally and mechanistically related protein families are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively. They have a wide range of functions including alpha-glucosidase, alpha-xylosidase, 6-alpha-glucosyltransferase, 3-alpha-isomaltosyltransferase, alpha-N-acetylgalactosaminidase, stachyose synthase, raffinose synthase, and alpha-1,4-glucan lyase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
SNU96820.1	6.35e-161	1	534	6	530
QIB59833.1	4.54e-159	1	534	6	533
BDA09953.1	4.16e-157	1	534	6	533
QCU01578.1	1.41e-147	46	531	71	544
CBL19648.1	9.05e-146	1	531	19	544

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000066	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004109_01499.