CAZyme Information: MGYG000004199_00219

Basic Information

• Lineage: Bacteria; Firmicutes; Bacilli; RFN20; CAG-449; CAG-449
• CAZyme ID: MGYG000004199_00219
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
609		71712.83	7.452

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004199	1652569	MAG	United Kingdom	Europe

• Gene Location: Start: 33577; End: 35406 Strand: -

Full Sequence      

MSEINFEKIDLLDGFWKDRYELNINASIPNIIKRFNDTRFKAIFFTYDNKDDSHHRAYDS
DVAKLIEAIAYYLVKDRKKYAEYEQICDQLIDSIATHQQKDGYFNSYFQVVRPNDILKNR
CDHELYNLGHLIEAAIAYDQATNKKVLLNVIKKYLEFVDEHFIKNNDVNFVTPGHEEIEL
ALLRLYKYTGDKKYFNMASFFLEKRGANEKDYYEEFANKYYAQDNAPIRELNSVEGHAVR
AMYLYDGMAKYADINKDKAIYSSLNKLYADLLTKQYITGSIGSYRAGEIFTIPHDLPPLT
AYSESCASIGEMMFLFDLYNLKHKKEYHDQIERILYNGFLSSTSLDGKAFFYENPLEIRL
KEINKETSVKDIWKQKLPITHRVELFECSCCPPNIARTIASISKYIYYEENNDIYINQFI
SSTLHFTNKEIVLKSQFPKEFKIEVEYKADSNNFLKIRLPWYSKKPIINVTGCKYLGVKN
GYLIFKNEKVNSKINIDFNVKPFFIEANPNNSYCQNKYALLYGMIVYCLEGIDNGENLNA
LFVKKTHDIKIEYNETYKMNTLSLEGYRKLDNGKEYSTYKYIKTTLKFIPYYCFANRETT
DMLVWVNKK

Enzyme Prediction     

No EC number prediction in MGYG000004199_00219.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	11	528	6.5e-141	0.9942748091603053

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3533	COG3533	3.34e-110	11	609	18	588	Uncharacterized conserved protein, DUF1680 family [Function unknown].
pfam07944	Glyco_hydro_127	6.00e-99	11	528	4	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG4225	YesR	0.006	83	194	12	100	Rhamnogalacturonyl hydrolase YesR [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QHQ63226.1	5.55e-128	4	605	9	621
AUS96918.1	2.56e-123	4	605	12	624
QTL99209.1	1.19e-113	59	608	76	651
ABX40978.1	1.18e-111	59	608	72	644
BCZ47937.1	1.90e-111	7	606	13	646

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4QJY_A	1.14e-96	59	609	76	648	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	3.81e-93	59	609	76	648	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
6EX6_A	1.05e-86	1	606	7	623	TheGH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482],6EX6_B The GH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482]
3WRE_A	6.42e-80	51	605	66	655	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	8.13e-80	51	605	66	655	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	3.51e-79	51	605	66	655	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000065	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004199_00219.