CAZyme Information: MGYG000004250_02441

Basic Information

• Species: Acetatifactor sp003447295
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Acetatifactor; Acetatifactor sp003447295
• CAZyme ID: MGYG000004250_02441
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase large subunit

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
639		74390.41	4.6796

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004250	3091301	MAG	United Kingdom	Europe

• Gene Location: Start: 62; End: 1981 Strand: +

Full Sequence      

MEADIRWLDDPQVFRVGQLPAHSDHPIYQDTEEAAEGRSSLVQSLDGTWEFAYSVNAKSR
PVDFYRENADSAQFDTIRVPGHIELAGYDQIHYINNMYPWEGKIYRRPAYTLGKGLAEGA
FSEAEYNPVGSYRKRFHLAEGLRGKRVIICFEGVEQAMYVWLNGHFVGYAEDSFTPSEFD
LTPYIKEKDNLLAVEVHKRSTAAFLEDQDFFRFFGIFRSVELYAKPQWHVEDLWAKPYFS
VEDGKGTLDAAIKISAEDEGQRPGKIHIRLSDSMGHKLLERTEWMKQEMEQTLHLKEELA
EKVIPWSHENPYLYQLEITLTDNAGEVVEVVPYKIGFRDFVLDPQDKVMKLNGERLVICG
VNRHEWNAASGRCISMDDMKWDIVCFKRNNINAVRTCHYPDCKEWYGLCDEAGIYVMAET
NLESHGSWQKMCDVEPSWNVPGSVPEWKEAVVDRARTNFECYKNHPSILFWSLGNESYAG
DDIAAMQQFFKEKDDLRLVHYEGVFHNKAYEDVISDMESRMYAYPQEIIDYLENDPKKPY
LLCEYMHDMGNSLGGMNSYMDLLDRFEMYQGGFIWDYIDQALWVKDEVTGRRVLRYGGDF
DDRPSDYEFSGNGILFADRSEKPAMQEVRYYYGTQNRNR

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	28	632	7.4e-145	0.6263297872340425

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10340	ebgA	3.01e-159	6	629	3	596	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	6.31e-142	11	630	20	623	beta-galactosidase.
pfam02836	Glyco_hydro_2_C	2.60e-130	346	633	5	298	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
COG3250	LacZ	2.62e-123	33	638	3	557	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam02837	Glyco_hydro_2_N	1.94e-30	42	226	1	169	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNM02889.1	0.0	1	639	1	639
ABX42393.1	8.21e-313	1	633	1	636
AQP40966.1	4.16e-304	1	633	1	630
CBK94938.1	4.98e-298	1	633	1	630
CBK90745.1	4.07e-297	1	633	1	630

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6S6Z_A	2.41e-117	9	632	5	593	Structureof beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_B Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_C Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_D Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_E Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_F Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_G Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_H Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8]
6SD0_A	2.45e-117	9	632	6	594	Structureof beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_B Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_C Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_D Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8]
1F4A_A	8.34e-101	9	630	15	620	E.COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4A_B E. COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4A_C E. COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4A_D E. COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4H_A E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli],1F4H_B E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli],1F4H_C E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli],1F4H_D E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli]
6CVM_A	8.34e-101	9	630	16	621	Atomicresolution cryo-EM structure of beta-galactosidase [Escherichia coli K-12],6CVM_B Atomic resolution cryo-EM structure of beta-galactosidase [Escherichia coli K-12],6CVM_C Atomic resolution cryo-EM structure of beta-galactosidase [Escherichia coli K-12],6CVM_D Atomic resolution cryo-EM structure of beta-galactosidase [Escherichia coli K-12],6X1Q_A 1.8 Angstrom resolution structure of b-galactosidase with a 200 kV cryoARM electron microscope [Escherichia coli K-12],6X1Q_B 1.8 Angstrom resolution structure of b-galactosidase with a 200 kV cryoARM electron microscope [Escherichia coli K-12],6X1Q_C 1.8 Angstrom resolution structure of b-galactosidase with a 200 kV cryoARM electron microscope [Escherichia coli K-12],6X1Q_D 1.8 Angstrom resolution structure of b-galactosidase with a 200 kV cryoARM electron microscope [Escherichia coli K-12]
5A1A_A	8.48e-101	9	630	16	621	2.2A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor [Escherichia coli K-12],5A1A_B 2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor [Escherichia coli K-12],5A1A_C 2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor [Escherichia coli K-12],5A1A_D 2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q48846	9.19e-288	1	633	1	622	Beta-galactosidase large subunit OS=Latilactobacillus sakei OX=1599 GN=lacL PE=3 SV=1
Q02603	1.75e-278	1	633	1	623	Beta-galactosidase large subunit OS=Leuconostoc lactis OX=1246 GN=lacL PE=1 SV=1
Q7WTB4	1.08e-277	1	633	1	625	Beta-galactosidase large subunit OS=Lactobacillus helveticus OX=1587 GN=lacL PE=2 SV=1
O07684	1.36e-273	1	633	1	625	Beta-galactosidase large subunit OS=Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) OX=272621 GN=lacL PE=3 SV=2
Q9K9C6	1.16e-213	3	632	5	614	Beta-galactosidase OS=Alkalihalobacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=lacZ PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000052	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004250_02441.